個人簡歷
博士、副教授。天津大學學士、碩士學位，上海交通大學博士學位，法國國家科學研究中心博士後。主持完成一項國家自然科學基金（基於耐高溫mutS蛋白、pfu DNA聚合酶、dU修飾寡核苷酸探針的SNP檢測技術），作為第二完成人參與一項國家973二級子項目（蛋白質複合物動態變化的分子機制與功能）和一項國家863探索項目（基於RNase H 酶促反應檢測SNP 的技術）。研究興趣主要集中在極端微生物DNA複製損傷修復及套用研究。已總結20篇SCI論文，其中第一作者10篇。另外獲得授權了5項關於SNP測定和基因克隆的國家發明專利。
研究方向
1) DNA損傷修復、DNA複製。重點研究極端嗜熱、嗜酸、嗜鹽微生物的DNA複製及調控機制，鹼基切除修復、核苷酸切除修復、同源重組修復等DNA損傷修復反應機制等。
2) 極端微生物合成的有重要工業套用價值的酶製劑的開發套用。從各種極端微生物中篩選澱粉酶、脂肪酶、蛋白酶等工業酶製劑。
3) 高通量基因克隆和重組蛋白製備技術研究。開發各種高通量基因克隆、蛋白製備技術，套用於生命科學研究和蛋白藥物篩選。
4) 核酸檢測與基因多態性分型。開發各種核酸檢測和基因多態性分型技術和檢測試劑盒，用於人類遺傳病分子診斷和家禽養殖等農牧業生產中的流行病檢測。
發表論文
[1] Lu Zheng, Liang Rubing, Liu Xipeng, Hou Jingli, Liu Jianhua RNase HIII from Chlamydophila pneumoniae can efficiently cleave double-stranded DNA carrying a chimeric ribonucleotide in the presence of manganese. Mol Microbiol. 2012 Mar;83(5):1080-1093.
[2] Liu Xipeng, Liu Jianhua Characterization of family IV UDG from Aeropyrum pernix and its application in hot-start PCR by family B DNA polymerase, 2011 Nov 8, PLoS One. 6(11): e27248.
[3] Daugeron MC, Lenstra TL, Frizzarin M, El Yacoubi B, Liu Xipeng, Baudin-Baillieu A, Lijnzaad P, Decourty L, Saveanu C, Jacquier A, Holstege FC, de Crécy-Lagard V, van Tilbeurgh H, Libri D Gcn4 misregulation reveals a direct role for the evolutionary conserved EKC/KEOPS in the t6A modification of tRNAs. Nucleic Acids Res. 2011 Aug;39(14):6148-6160.
[4] Liu Xipeng, Liu Jianhua. The terminal 5' phosphate and proximate phosphorothioate promote ligation-independent cloning. Protein Sci. 2010 May;19(5): 967-973.
[5] Liu Xipeng, Hou Jingli, Liu Jianhua. A novel single nucleotide polymorphism detection of a double-stranded DNA target by a ribonucleotide-carrying molecular beacon and thermostable RNase HII. Anal Biochem. 2010 Mar 1;398(1):83-92.
[6] Liu Xipeng, Li Chunpeng, Hou Jingli, Liu Yufen, Liang Rubing, Liu Jianhua. Expression and characterization of thymine-DNA glycosylase from Aeropyrum pernix. Protein Expr Purif. 2010 Mar;70(1):1-6.
[7] Liu Xipeng, Zhang Yang, Liang Rubing, Hou Jingli, Liu Jianhua. Characterization of the 3' exonuclease of Chlamydophila pneumoniae endonuclease IV on double-stranded DNA and the RNA strand of RNA/DNA hybrid. Biochem Biophys Res Commun. 2007 Oct 5; 361(4):987-993.
[8] Liu Xipeng, Hou Jingli, Liu Jianhua. Chlamydial DNA polymerase I can bypass lesions in vitro. Biochem Biophys Res Commun. 2006 Jul 7; 345(3):1083-1091.
[9] Liu Xipeng, Liu Jianhua. The mechanism of base excision repair in Chlamydiophila pneumoniae. DNA Repair 2005 Nov 21; 4(11):1295-1305.
[10] Liu Xipeng, Liu Jianhua. Chlamydia pneumoniae AP endonuclease IV could cleave AP sites of double- and single-stranded DNA. Biochim Biophys Acta. 2005 Dec 1; 1753(2):217-225.
[11] Liu Xipeng, Liu Jianhua. Signal peptide does not inhibit binding of biotin to streptavidin. Biotechnol Lett. 2005 Aug; 27(15):1067-1073.
[12] Liang Rubing, Liu Xipeng, Liu Jianhua, Ren Qiushi, Liang Peiji, Lin Zhixin, Xie Xiangming. A T7-expression system under temperature control could create temperature-sensitive phenotype of target gene in Escherichia coli. J Microbiol Methods. 2007 Mar; 68(3):497-506.
[13] Liang Rubing, Liu Xipeng, Pei Dongli, Liu Jianhua. Biochemical characterization and functional complementation of ribonuclease HII and ribonuclease HIII from Chlamydophila pneumoniae AR39. Microbiology（UK）. 2007 Mar; 153(Pt 3):787-793.
[14] Hou Jingli, Liu Xipeng, Pei Dongli, Liu Jianhua. RNase HII from Chlamydia pneumoniae discriminates mismatches incorporation into DNA-rN1-DNA/DNA duplexes. Biochem Biophys Res Commun. 2007 May 18; 356(4):988-992.
[15] Hou Jingli, Liu Xipeng, Zheng Yan, Liu Jianhua, DuanTao. Molecular beacons for isothermal fluorescence enhancement by the cleavage of RNase HII from Chlamydia pneumoniae. Anal Biochem. 2007 Dec 15; 371(2):162-166.
[16] Hou Jingli, Liu Xipeng, Zheng Yan, Liu Jianhua. A Method for HLA Genotyping Using the Specific Cleavage of DNA-rN1-DNA/DNA with RNase HII from Chlamydia pneumoniae. Oligonucleotides, 2007 Winter, 17(4):433-444.
