主要研究經歷:
主要從事微生物技術在農產品安全生產中的套用方面的研究。作為主要貢獻人參與了農藥殘留微生物降解技術的研發，建立了國內最大的農藥殘留降解微生物菌種資源庫，提出了農藥殘留微生物降解田間套用標準，並在水稻、蔬菜和茶葉等農作物上進行了大規模的試驗示範，項目獲得2005年度國家科技進步二等獎。近年來開展了新型糖苷水解酶資源開發以及生防菌粘細菌在植物病害防治方面的研究和套用等工作，尤其是生防粘細菌EGB在枯萎病和稻瘟病防治方面取得了良好的研究成果。先後主持完成國家“863”計畫項目、國家自然科學基金項目多項。2005-2007年在日本京都大學進行了蛋白質晶體結構解析方面的研究工作，熟悉蛋白質晶體培養、X-衍射數據分析析，解析了海藻糖降解途徑中的鼠李糖苷酶的結構。發表論文100餘篇，其中在Appl. Environ. Microbiol., Appl Microbiol Biotech.,Biol Control等雜誌上發表SCI論文50餘篇。
•2006/06-至今，南京農業大學，生命科學學院，教授
•2005/10-2007/02，日本國京都大學，農學部，博士後，合作導師 Kousaku Murata
•2003/05-2005/08，中國科學院南京土壤研究所，博士後，合作導師趙其國院士
•2002/05-2006/06，南京農業大學，生命科學學院，副教授
•2000/08-2002/05，南京農業大學，資源與環境學院，講師
研究方向:
•1. 生物降解與生物轉化; BiodegradationandBioconversion.
•2. 酶資源與蛋白質工程;Enzyme resources and protein engineering
•3. 土傳病害真菌防治; Biocontrol ofplant fungal disease
發表論文：
•1. Jie Zhou, Zhoukun Li, Han Zhang, Jiale Wu, Xianfeng Ye, Weiliang Dong, Min Jiang, Yan Huang,Zhongli Cui*, A Novel Maltogenic Amylase CoMA fromCorallococcussp. EGB Catalyzes the Conversion of Maltooligosaccharidesand Soluble Starch to Maltose.Appl Environ Microb, 2018.
•2. Qiongzhen Chen; Yajuan Duan; Zhoukun Li;Zhongli Cui*; Weidong Liu, Yan Huang*. Crystal structure of p-nitrophenol 4-monooxygenase PnpA fromPseudomonas putidaDLL-E4: the key enzyme involved in p-nitrophenol degradation.Biochemical and Biophysical Research Communications, BBRC. 2018
•3. Zhoukun Li #,Ting Wang #, Xue Luo, Xiangmin Li, Chengyao Xia, Yuqiang Zhao, Xianfeng Ye, Yan Huang, Xiangyang Gu, Hui Cao,Zhongli Cui*, Jiaqin Fan *. Biocontrol potential ofMyxococcussp. strain BS against bacterial soft rot of calla lily caused byPectobacterium carotovorum.Biol Control, 2018, 126: 36-44
•4. Zhoukun Li, Xianfeng Ye, Peilin Chen, Kai Ji, Jie Zhou, Fei Wang, Weiliang Dong, Yan Huang, Zhengguang Zhang,Zhongli Cui*, Antifungal potential ofCorallococcussp. strain EGB against plant pathogenic fungi,Biol Control, 2017, 110: 10-17
•5. Jie Zhou#, Zhoukun Li#, Jiale Wu, Lifeng Li, Ding Li, Xianfeng Ye, Xue Luo, Yan Huang,Zhongli Cui*, Hui Cao*, Functional analysis of a novel β-(1,3)-glucanase fromCorallococcussp. EGB containing a fascin-like module,Appl Environ Microb, 2017, 83(16): e01016-17
•6. Weiliang Dong#, Fei Wang#, Fei Huang#, Yicheng Wang, Jie Zhou, Xianfeng Ye, Zhoukun Li, Yin Hou, Yan Huang, Jiangfeng Ma, Ming Jiang,Zhongli Cui*. Metabolic pathway involved in 6-chloro-2-benzoxazolinone degradation byPigmentiphagasp. strain DL-8 and identification of the novel metal-dependent hydrolase CbaA.Appl. Environ. Microbiol, 2016, 82(14): 4169-4179
•7. Zhoukun Li, Jiale Wu, Biying Zhang, Fei Wang, Xianfeng Ye, Yan Huang, Qiang Huang,Zhongli Cui*. AmyM, a novel maltohexaose-forming α-amylase fromCorallococcussp. strain EGB.Appl. Environ. Microbiol, 2015, 81(6): 1977-1987
•8. Fei Wang, Jie Zhou, Zhoukun Li, Weiliang Dong, Ying Hou, Yan Huang,Zhongli Cui*. Involvement of the cytochrome P450 system EthBAD in the N-deethoxymethylation of acetochlor byRhodococcussp. strain T3-1.Appl. Environ. Microbiol, 2015, 81(6): 2182-2188
•9. Weiliang Dong#, Qiongzhen Chen#, Ying Hou, Shuhuan Li, Kai Zhuang, Fei Huang, Jie Zhou, Zhoukun Li, Jue Wang, Lei Fu, Zhengguang Zhang, Yan Huang, Fei Wang,Zhongli Cui*. Metabolic Pathway Involved in 2-Methyl-6-Ethylaniline Degradation bySphingobiumsp. Strain MEA3-1 and Cloning of the Novel Flavin-Dependent Monooxygenase System meaBA.Appl. Environ. Microbiol, 2015, 81(24): 8254-8264
•10. Fei Wang, Ying Hou, Jie Zhou, Zhoukun Li, Yan Huang,Zhongli Cui*. Purification of an amide hydrolase DamH fromDelftiasp. T3-6 and its gene cloning, expression, and biochemical characterization.Appl Microbiol Biotech, 2014, 98(17): 7491-7499
•11. Jingquan Li#, Yan Huang#, Ying Hou, Xiangmin Li, Hui Cao,Zhongli Cui*. Novel Gene Clusters and Metabolic Pathway Involved in 3,5,6-Trichloro-2-Pyridinol Degradation byRalstoniasp.T6.Appl. Environ. Microbiol. 2013, 79(23):7445-53
•12.Ying Hou, Jian Tao, Wenjing Shen, Juan Liu, Jingquan Li, Yongfeng Li, Hui Cao &Zhongli Cui. Isolation of the fenoxaprop-ethyl (FE)-degrading bacteriumRhodococcussp. T1, and cloning of FE hydrolase genefeh.FEMS Microbiol Lett. 2011,323(2):196-203.
所獲獎項：
•教育部技術發明獎，土壤農藥污染微生物修復技術的研究與套用，二等，2007，第二獲獎人 (李順鵬，崔中利，何健，洪青，蔣建東，管曉進)
•國家科技進步獎，農藥殘留微生物降解技術的研究與套用，二等，2005，第二獲獎人（10）(李順鵬、崔中利、沈 標、劉 智、何 健、楊興明、王新華、張瑞福、蔣建東、洪 青)
盤點農業部重點實驗室主任之二
| 根據《農業部重點實驗室管理辦法》的有關規定，經研究，農業部同意聘任林敏等228位專家為農業部重點實驗室主任，讓我們來盤點一下這些專家。 |
