喬建軍:天津大學化工學院教授。
研究方向:
研究方向為微生物合成生物學,主要針對綠色食品防腐劑Nisin 合成的關鍵問題進行系統的研究,同時也利用合成生物技術進行其他天然產物的高效合成研究。
講授課程:
本科生教學:生物化學、生物化學工程基礎(重點講述生物化學及微生物在工業上的套用);
研究生教學:基因(英文)
學術兼職:
中國輕工業合成生物技術重點實驗室主任
系統生物工程教育部重點實驗室副主任
天津市微生物學會常務理事
天津市食用菌協會 副會長
天津大學學報編委
民盟天津大學委員會委員
天津市南開區政協委員
學術成就:
教育部新世紀人才、天津市131第一層次人才、天津大學北洋青年學者。
利用合成生物技術構建了多個天然產物的高效生產菌株;通過基因組測序、轉錄組分析、蛋白質組分析、小RNA深度測序分析,揭示了高產nisin的乳酸乳球菌在酸應激下可能的調控機制,鑑定特異轉錄因子,揭示其調控機制。在大腸桿菌和乳酸乳球菌中表達了大量的耐酸基因,涉及細胞壁、細胞膜、分子伴侶、質子轉運、胺基酸代謝等方面,構建得到了高耐酸的菌株。目前希望能夠更加深入的理解耐酸機制並且套用合成生物學技術構建更為理性的耐酸網路;建立了廢棄菌糠、米糠和豆粕的處理工藝,針對產物和菌種需求設計並構建相應的菌株,降低發酵生產成本。
科研項目:
主持完成國家自然科學基金、國家973計畫子課題、國家重大863項目子課題、國家支撐計畫子課題、天津市自然科學重點基金等國家省部級項目十餘項。目前主持國家重點研發計畫項目1項,任首席科學家;主持國防科技項目1項;主持國家自然科學基金2項,合作企業項目2項。實到研究經費共1千餘萬元。
論著專利:
在Science、Chem Soc Rev、Meta Eng、Appl Environ Microbiol等期刊發表Sci論文50餘篇。
1.Liuxue,…Qiao Jianjun*, Zhao Guangrong*, Convergent engineering of syntrophic Escherichia coli coculture for efficient production of glycosides, Metabolic Engineering, 2018. 待出版(IF 8.2)
2.Wu Hao, Zhao Yue, …Qiao Jianjun*, Quantitative proteomics of Lactococcus lactis F44 under cross-stress of low pH and lactate, J Dairy Science, 2018, (已接收)
3. Wuhao, Miao sen, Qiao jianjun*. Enhancing nisin yield by engineering a small noncodding RNA anti41 and inhibiting the expression of glnR in Lactococcus lactis F44", Biotechnology Letters, 2018, 待出版
4. Liu jiaheng, Li Huiling, Qiao Jianjun*. Redox cofactor engineering in industrial microorganisms: strategies, recent applications and future directions,Journal of Industrial Microbiology & Biotechnology,2018,DOI 10.1007/s10295-018-2031-7.(特邀綜述)
5. Zhu H, Tian L, Zhang L, Bi J, Song Q, Yang H, Qiao J*. Preparation, characterization and antioxidant activity of polysaccharide from spent Lentinus edodes substrate. Int J Biol Macromol. 2018 Feb 8. pii: S0141-8130(17)32974-4. doi: 10.1016/j.ijbiomac.2018.01.196
6. Hao Wu; shunyi Song; Kairen Tian; Dandan Zhou; Binbin Wang; Jiaheng Liu; Hongji Zhu; Jianjun Qiao*, A novel small RNA S042 increases acid tolerance in Lactococcus lactis F44 , BBRC, 2018 (已接收)
7. Wu H, Liu J, Miao S, Zhao Y, Zhu H, Qiao M, Saris PEJ, Qiao J*. Contribution of YthA, a PspC Family Transcriptional Regulator of Lactococcus lactis F44 Acid Tolerance and Nisin Yield: a Transcriptomic Approach. Appl Environ Microbiol. 2018 Mar 1;84(6). pii: e02483-17. doi: 10.1128/AEM.02483-17. Print 2018 Mar 15.
8. Binbin Wang, Huawei Zhang,Dongmei Liang, Panlong Hao, Yanni Li, and Jianjun Qiao*,Acid or erythromycin stress significantly improves transformation efficiency through regulating expression of DNA binding proteins in Lactococcus lactis F44 . J Dairy Science. 2017, 100: 9532–9538
9. Liu J, Zhou J, Wang L, Ma Z, Zhao G, Ge Z, Zhu H, Qiao J*. Improving nitrogen source utilization from defatted soybean meal for nisin production by enhancing proteolytic function of Lactococcus lactis F44. Sci Rep. 2017 Jul 21;7(1):6189. doi: 10.1038/s41598-017-06537-w.
10. Qi J, Caiyin Q, Wu H, Tian K, Wang B, Li Y, Qiao J*. The novel sRNA s015 improves nisin yield by increasing acid tolerance of Lactococcus lactis F44. Appl Microbiol Biotechnol. 2017, 101(16):6483-6493
11. Tang ZK, Li XM, Pang AP, Lin CY, Zhang Y, Zhang J, Qiao J*, Zhao GR*. Characterization of three pathway-specific regulators for high production of monensin in Streptomyces cinnamonensis. Appl Microbiol Biotechnol. 2017 Aug;101(15):6083-6097.
12. Hao P, Liang D, Cao L, Qiao B, Wu H, Caiyin Q, Zhu H, Qiao J*. Promoting acid resistance and nisin yield of Lactococcus lactis F44 by genetically increasing D-Asp amidation level inside cell wall. Appl Microbiol Biotechnol. 2017 Aug;101(15):6137-6153.
13. Liu J, Ma Z, Zhu H, Caiyin Q, Liang D, Wu H, Huang X, Qiao J*. Improving xylose utilization of defatted rice bran for nisin production by overexpression of a xylose transcriptional regulator in Lactococcus lactis. Bioresour Technol. 2017 Aug;238:690-697. doi: 10.1016/j.biortech.2017.04.076. Epub 2017 Apr 22.
14. Ruan HH*, Zhang Z, Wang SY, Nickels LM, Tian L, Qiao JJ*, Zhu J. Tumor Necrosis Factor Receptor-Associated Factor 6 (TRAF6) Mediates Ubiquitination-Dependent STAT3 Activation upon Salmonella enterica Serovar Typhimurium Infection. Infect Immun. 2017 Jul 19;85(8). pii: e00081-17. doi: 10.1128/IAI.00081-17. Print 2017 Aug.
15. Xie ZX, Li BZ, Mitchell LA, Wu Y, Qi X, Jin Z, Jia B, Wang X, Zeng BX, Liu HM, Wu XL, Feng Q, Zhang WZ, Liu W, Ding MZ, Li X, Zhao GR, Qiao JJ, Cheng JS, Zhao M, Kuang Z, Wang X, Martin JA, Stracquadanio G, Yang K, Bai X, Zhao J, Hu ML, Lin QH, Zhang WQ, Shen MH, Chen S, Su W, Wang EX, Guo R, Zhai F, Guo XJ, Du HX, Zhu JQ, Song TQ, Dai JJ, Li FF, Jiang GZ, Han SL, Liu SY, Yu ZC, Yang XN, Chen K, Hu C, Li DS, Jia N, Liu Y, Wang LT, Wang S, Wei XT, Fu MQ, Qu LM, Xin SY, Liu T, Tian KR, Li XN, Zhang JH, Song LX, Liu JG, Lv JF, Xu H, Tao R, Wang Y, Zhang TT, Deng YX, Wang YR, Li T, Ye GX, Xu XR, Xia ZB, Zhang W, Yang SL, Liu YL, Ding WQ, Liu ZN, Zhu JQ, Liu NZ, Walker R, Luo Y, Wang Y, Shen Y, Yang H, Cai Y, Ma PS, Zhang CT, Bader JS, Boeke JD, Yuan YJ*. "Perfect" designer chromosome V and behavior of a ring derivative. Science. 2017 Mar 10;355(6329). pii: eaaf4704. doi: 10.1126/science.aaf4704..