山形達也

基本情況

姓名：山形達也
任教專業：醫學-中藥類
在職情況：在職
性別：男
所在院系：
所教課程：
研究方向：1. Ito, M., and Yamagata, T., A novel glycosphingolipid-degrading enzyme cleaves the linkage between the oligosaccharide and ceramide of neutral and acidic glycosphingolipids. J. Biol. Chem., 261, 14278-14282 (1986) 2. Ito, M., Hirabayashi, Y., and Yamagata, T., Substrate specificity of endo-b-galactosidases from Flavobacterium keratolyticus and Escherichia freundii is different from that of Pseudomonas sp. J. Biochem., 100, 773-780 (1986) 3. Ohara, T., and Yamagata, T., Isolation and characterization of galactose-binding proteins from new-born mice. Biochim. Biophys. Acta, 884, 344-354 (1986) 4. Yamagata, S., and Yamagata, Y., The staining of type V collagen obtained from FBJ virus-induced osteosarcoma with concanavalin A. Biochem. Biophy. Res. Commun., 136, 857-862 (1986) 5. Higashi, H., Hirabayashi, Y., Ito, M., Yamagata, T., Matsumoto, M., Ueda, S., and Kato S., Immunostaining on thin-layer chromatograms of oligosaccharides released from gangliosides by endoglycoceramidase. J. Biochem., 102, 291-296 (1987) 6. Hirabayashi, J., Oda, Y., Ohara, T., Yamagata, T., and Kasai, K., Immunological cross-reactivity between β-galactoside-binding lectins of vertebrates. Possible relationship of antigenecity to oligomeric structure. Biochim. Biophys. Acta, 916, 321-327 (1987) 7. Mikawa, T., Sashida, R., Ito, M., and Yamagata, T., Rhodocuccus sp. producing endoglycoceramidase. Mitsubishi Chemical R & D Review, 1, 34-39 (1987)(in Japanese) 8. Shimamura, M., Hayase, T., Ito, M., Rasilo, M.-L., and Yamagata, T., Characterization of a major neutral glycolipid in pc12 cells as III3galα-globotriaosylceramide by the method for determing glycosphingolipid saccharide sequence with endoglycoceramidase. J. Biol. Chem., 263, 12124-12128 (1988) 9. Rasilo M.-L., and Yamagata, T., Occurrence of glucose polymer in undifferentiated PC12 cells. FEBS letters, 227, 197-194 (1988) 10. Rasilo M.-L., and Yamagata, T., Characterization of a glucose polymer from PC12 cells and neuronal cells of rat embryo. J. Biochem., 104, 742-754 (1988) 11. Ito, M., and Yamagata, T., Purification and characterization of glycosphingolipid-specific endoglycosidases (endoglycoceramidases) from a mutant strain of Rhodococcus sp. J. Biol. Chem., 264, 9510-9519 (1989) 12. Fukaya, N., Ito, M., iwata, H., and Yamagata, T., Characterization of the glycosphingolipids of pig cortical bone and cartilage. Biochim. Biophys. Acta, 1004, 108-116 (1989) 13. Rasilo, M.-L., Ito, M., and Yamagata, T., Liberation of oligosaccharides from glycosphingolipids on PC12 cell surface with endoglycoceramidase. Biochem. Biophys. Res. Commun., 162, 1093-1099 (1989) 14. Rasilo, M.-L., and Yamagata, T., The glucose polymer of PC12 cells is susceptible to trypsinization. Biochem. Biophys. Res. Commun., 159, 1-6 (1989) 15. Higashi, H., Ito, M., Fukaya, N., Yamagata, S., and Yamagata, T., Two-dimensional mapping by the high-performance liquid chromatography of oligosaccharides released from glycosphingolipids by endoglycoceramidase. Anal. Biochem., 186, 355-362 (1990) 16. Kubo, T., Hayase, T., Morimoto, Y., Kondo, J., Matsumoto, R., Nagai, K., and Yamagata, T., Structural analysis of the carbohydrate chain of recombinant apolipoprotein E. Mitsubihsi Kasei R & D Review, 4, 1-7 (1990)(in Japanese) 17. Ito, M., Ikegami, Y., and Yamagata, T., Activator proteins for glycosphingolipid hydrolysis by endoglycoceramidases. J. Biol. Chem., 266, 7919-7926 (1991) 18. Shimamura, M., Oku, M., and Yamagata, T., The retarded rate of acid-catalyzed solvolysis of glycoside bonds between reducing- end glycose residue and ceramide in glycosphingolipids compared with that of glycoside bonds between hexopyranosides. FEBS letters, 290, 213-215 (1991) 19. Ito, M., Ikegami, Y., Omori, A., and Yamagata, T., Conversion of endoglycoceramidase-activator II by trypsin to the 27.9 kDa polypeptide possessing full activity: Purification of activator for endoglycoceramidase by trypsin treatment followed by trypsin-inhibitor agarose column application. J. Biochem. 110, 328-332 (1991) 20. Yamagata, T., and YAMAZAKI, K., Implanting mouse embryo stain with a LNF-I bearing fluorescent probe at their mural trophectodermal side. Biochem. Biophys. Res. Commun., 181, 1004-1009 (1991) 21. Higashi, H., Omori, A., and Yamagata, T. Calmodulin: A ganglioside-binding protein. J. Biol. Chem., 267, 9831-9838 (1992) 22. Higashi, H., and Yamagata, T., Mechanism for ganglioside-mediated modulation of calmodulin-dependent enzyme. J. Biol. Chem., 267, 9839-9843 (1992) 23. Shimamura, M., Oku, M., and Yamagata, T., Presence of murine fetal liver cells capable of being induced to differentiate in vitro into T cell receptor αβ positive cells. Eur. J. Immunol., 22, 2159-2164 (1992) 24. Shimamura, M., Oku, M., Ohta, S., and Yamagata, T., Hematopoietic cell lines capable of colonizing the thymus following in vivo transfer expressed T cell receptor δ gene immature mRNA. Immunol. 77, 369-376 (1992) 25. Shimamura, M., Oku, M., and Yamagata, T., A novel ganglioside expressed by mouse hematopoietic cell lines. J. Biol. Chem., 267, 18810-18813 (1992) 26. Higashi, H., and Yamagata, T., Full activation without calmodulin of calmodulin-dependent cyclic nucleotide phosphodiesterase by acidic glycosphingolipids; GM3, sialosylneolactotetraosylceramide and sulfatide. FEBS letters, 314, 53-57 (1992) 27. Ito, M., Ikegami, Y., and Yamagata, T., Kinetics of endoglycoceramidase action toward cell-surface glycosphingolipids of erythrocytes. Eur. J. Biochem., 218, 645-649 (1993) 28. Ito, M., Ikegami, Y., Tai, T., and Yamagata, T., Specific hydrolysis of intact erythrocyte cell-surface glycosphingolipids by endoglycoceramidase. Eur. J. Biochem., 218, 637-643 (1993) 29. Ponce, R.H., Yanagimachi, R., Urch, U.A., Yamagata, T., and Ito, M., Retention of hamster oolema fusibility with spermatozoa after various enzyme treatments: A search for the molecules involved in sperm-egg fusion. Zygotes, 1, 163-171 (1993) 30. Shimamura, M., Shibuya, N., Ito, M., and Yamagata, T., Repulsive contribution of surface sialic acid residues to cell adhesion to substratum. Biochem. Mol. Biol. Internatl., 33, 871-878 (1994) 31. Muramoto, K., Kawahara, M., Kobayashi, K., Ito, M., Yamagata, T., and Koroda, Y., Endoglycoceramidase treatment inhibits synchorous oscillations of intercellular Ca2+ in cultured cortical neuron. Biochem. Biophys. Res. Commun., 202, 398-402 (1994) 32. Li. J., Ito, M., Zhang, G., and Yamagata, T., The hydrolysis of cell surface glycoshingolipids by endoglycoceramidase reduces epidermal growth factor receptor phosphorylation in A431 cells. Glycobiology, 5, 343-350 (1995) 33. Nagatsuka, Y., Watari, S., Yasuda, T., Higashi, H., Yamagata, T., and Ono, Y., Production of human monoclonal antibodies to i blood group by EBV-induced transformation: Possible presence of a new glycolipid in cord blood cell membranes and human hematopoietic cell lines. Immunol. Lett., 46, 93-100 (1995) 34. Miura, R.O., Yamagata, S., Harada, T., Miura, Y., and Yamagata, T., Analysis of glycosaminoglycan degrading enzymes by substrate gel electrophoresis (Zymography). Anal. Biochem., 225, 333-340 (1995) 35. Miura, Y., Arai, T., and Yamagata, T., Synthesis of amphiphilic lactosides that possess a lactosylceramide-mimicking N-acyl structure. Carbohydrate Res., 289, 193-199 (1996) 36. Higashi, H., Yoshida, S., Sato, K., and Yamagata, T., Interaction of ganglioside and specific peptide sequences as a mechanism for modulation of calmodulin-dependent enzymes by gangliosides. J. Biochem., 120, 66-73 (1996) 37. Nagatsuka, Y., Yamaki, M., Watarai, S., Yasuda, T., Higashi, H., Yamagata, T., and Ono, Y., The Epstein-Barr virus (EBV) alters the B cell glycolipid which is recognized by the human monoclonal antibody to i-blood antigen. Virus Research, 43, 57-68 (1996) 38. Hyuga, S., Yamagata, S., Tai, T., and Yamagata, T., Inhibition of highly metastatic FBJ-LL cell migration by ganglioside GD1a highly expressed in poorly metastatic FBJ-S1 cells. Biochem. Biophys. Res. Commun., 231, 340-343 (1997) 39. Kikkawa, F., Tamakoshi, K., Nawa, A., Shibata, K., Yamagata, S., Yamagata, T., and Suganuma, N., Positive correlation between inhibitors of matrix metalloproteinase 1 and matrix metalloproteinases in malignant ovarian tumor tissues. J Cancer Lett., 120, 109-115 (1997) 40. Tamakoshi, K., Kikkawa, F., Yamagata, S., Yamagata, T., and Tomoda, Y., Hyaluronidase activity in gynecologic cancer tissue with different metastatic forms. British J. Cancer, 75, 1807-1811 (1997) 41. Suzuki, T., Horie, G., Yamazaki, Y., Kawabe, K., Masiuda, H., Miyamoto, D., Matsuda, M., Nishimura, S.,Yamagata, T., Ito, T., Kida, H., Kawaoka, Y., and Suzuki, T., Swine influenza virus strains recongnize sialylsugar chains containing the molecular species of sialic acid predominatly present in the swine tracheal epithelium. 42. Miura, Y., and Yamagata, T., Glycosylation of lactosylceramide analogs in animal cells; Amphipathic disaccharide primers for glycosphingolipid synthesis. 43. Podyma, K.A., Yamagata, S., Sakata, K., and Yamagata, T., Difference of hyaluronidase produced by human tumor cell lines with hyaluronidase present in human serum.as revealed by zymography Biochem. Biophys. Res. Commun., 241, 446-450 (1997) 44. Nakajima, H., Miura, Y., and Yamagata, T., Glycosylation of amphipathic lactoside primers with consequent inhibition of endogenous glysosphingolipid synthesis. J. Biochem., 124, 148-156 (1998) 45. Ji, L., Ito, M., Zhang, G., Hirabayashi, Y., Inokuchi, J., and Yamagata , T., Effects of endoglycoceramidase of D-threo-1-phenyl-2-decanoylamino-3-morpholino-1-propanol on glucose uptake, glycolysis, and mitochondrial respiration in HL60 cells. Arch Biochem. Biophys., 359, 107-14 (1998) 46. Miura, Y., Arai, T., Ohtake, A., Ito, M., Yamamoto, K., and Yamagata, T., Requirement for a different hydrophobic moiety and reliable chromogenic substrate for endo-type glycosylceramidases Glycobiology, 9, 957-960 (1999) 47. Nagaya, N., Yamagata, T., Yamagata, S., Iyoda, K., Ito, H., Hasegawa, Y., and Iwata, H., Examination of synovial fluid and serum hyaluronidase activity as a joint marker in rheumatoid arthritis and osteoarthritis patients (by zymography) Ann. Rheum. Dis., 58, 186-188 (1999) 48. Hyuga, S., Yamagata, S., Takatsu, Y., Hyuga, M., NAKANISHI, H., Furukawa, K. and Yamagata, T., Suppression of FBJ-LL cell adhesion to vitronectin by ganglioside GD1a and loss of metastatic capacity. International J. Cancer, 83, 685-691 (1999) 49. Hyuga,S., KAWASAKI, N., Hashimoto, O., Hyuga, M., Ohta, M., Yamagata, S., Yamagata, T., and Hayakawa, T., Possible role of hepatosyte growth gactor/scatter factor and activin A produced by the target organ in liver metastasis. Cancer Lett., 153, 137-143 (2000) 50 Kasuya, Maria C. Z., Wang, L. X., Lee, Y. C, Mitsuki, M., Nakajima, H.,Miura, Y., Sato, T., Hatanaka, K., Yamagata, S., and Yamagata, T., Azido glycoside primer: A versatile building block for the biocombinatorial synthesis of glycosphingolipid analogues. Carbohydr. Res. 329, 755-763 (2000) 51. Hyuga, S., Kawasaki, N., Ohta, M., Shibayama, R., Kawanishi, T., Yamagata, S., Yamagata, T., and Hayakawa, T., Ganglioside GD1a inhibits the HGF/SF-induced motility and scattering of cancer cells through suppression of tyrosine-phosphorylation of c-Met. International J. Cancer, 94, 328-334 (2001) 52. Nadanaka, S., Sato, C., Kitajima, K., Katagiri, K., Irie, S., and Yamagata, T., Occurrence of Oligosialic Acids on Integrin alpha5 Subunit and Their Involvement in Cell Adhesion to Fibronectin. J. Biol. Chem. 276, 33657-33664 (2001)

個人簡介

山形達也(Yamagata Tatsuya) 教授 博士生導師 [性 別]： 男 [國 籍]： 日本 [出生日期]： 1937年1月1日 [出 生 地]： 東京都目黑區 [學歷] 1960年3月 東京大學理學部化學科畢業 1962年3月 東京大學大學院化學研究科生物化學理學碩士畢業 1968年2月 名古屋大學理學博士畢業 [簡歷] 1962年4月-1968年2月 名古屋大學理學部化學科助教 1968年3月-1973年4月 名古屋大學理學部化學科講師 1969年8月-1971年10月 美國芝加哥大學醫學部小兒科研究員 1973年5月-1973年10月 名古屋大學理學部化學科副教授 1973年11月-1982年1月 三菱化成生命科學研究所發生生物學研究室主任研究員 1982年2月-1987年3月 三菱化成生命科學研究所發展研究室室長 1987年4月-1993年3月 三菱化成生命科學研究所複合糖質研究室室長 1988年4月-1993年3月 兼任三菱化成生命科學研究所細胞識別研究部部長 1993年4月-1998年3月 東京工業大學生命理工學部客座教授 1998年4月-2003年3月 日本皮革研究所?糖鎖情報工學部?理事研究員，教授 2001年6月-2003年3月 瀋陽藥科大學客座教授 2003年3月 瀋陽藥科大學教授，博士生導師 [獲獎] 1969年 日本生化學會獎 [所屬學會] 1961年- 日本生化學會 評議員 1985年- 日本糖質學會 名譽會員 1989年-1995年 FCCA (Forum: Carbohydrates Coming of Age） 創立者，幹事長 1989年-1999年 Trends in Glycoscience and Glycotechnology 主編 1998年-2003年 東京糖鎖研究會GlycoTOKYO 會長 1996年- Society for Glycobiology 1997年- American Soc. Biochem. & Mol. Biol. 1996年- 日本癌學會 [主要研究課題]： 1． 硫酸軟骨素分解酶及硫酸軟骨素的構造 2． 胚胎髮生研究及蛋白聚糖M的發現 3． V型膠原蛋白的構造 4． 動物凝集素的作用 5． 糖脂分解酶的發現及套用 6． 糖脂機能的研究 7． 腫瘤的轉移與糖脂的機能 8．利用糖鏈引物用細胞製備糖鏈 [研究方向]： 1． 雖然人們已經清楚腫瘤是由基因突變所引起的，但是目前採用基因治療仍然很困難。為了防治癌症患者的死亡，本實驗室致力於腫瘤轉移機制的研究，尤其是對神經節苷脂抑制腫瘤轉移性的細胞信號轉導通路的研究，希望能夠發現抑制腫瘤轉移的藥物。 2． 細胞表面的糖鏈與細胞的識別，粘附，移動，分裂有著密切的關係，為了研究糖鏈的機能，本實驗室致力於利用細胞合成各種糖鏈的研究。我們認為如果能清楚糖鏈調控細胞功能的機理，不僅能夠解釋一些疾病的病因，而且還能開發出相應的治療藥物。 [主要論文]：（發表於1986年後） 1. Ito, M., and Yamagata, T., A novel glycosphingolipid-degrading enzyme cleaves the linkage between the oligosaccharide and ceramide of neutral and acidic glycosphingolipids. J. Biol. Chem., 261, 14278-14282 (1986) 2. Ito, M., Hirabayashi, Y., and Yamagata, T., Substrate specificity of endo-b-galactosidases from Flavobacterium keratolyticus and Escherichia freundii is different from that of Pseudomonas sp. J. Biochem., 100, 773-780 (1986) 3. Ohara, T., and Yamagata, T., Isolation and characterization of galactose-binding proteins from new-born mice. Biochim. Biophys. Acta, 884, 344-354 (1986) 4. Yamagata, S., and Yamagata, Y., The staining of type V collagen obtained from FBJ virus-induced osteosarcoma with concanavalin A. Biochem. Biophy. Res. Commun., 136, 857-862 (1986) 5. Higashi, H., Hirabayashi, Y., Ito, M., Yamagata, T., Matsumoto, M., Ueda, S., and Kato S., Immunostaining on thin-layer chromatograms of oligosaccharides released from gangliosides by endoglycoceramidase. J. Biochem., 102, 291-296 (1987) 6. Hirabayashi, J., Oda, Y., Ohara, T., Yamagata, T., and Kasai, K., Immunological cross-reactivity between β-galactoside-binding lectins of vertebrates. Possible relationship of antigenecity to oligomeric structure. Biochim. Biophys. Acta, 916, 321-327 (1987) 7. Mikawa, T., Sashida, R., Ito, M., and Yamagata, T., Rhodocuccus sp. producing endoglycoceramidase. Mitsubishi Chemical R & D Review, 1, 34-39 (1987)(in Japanese) 8. Shimamura, M., Hayase, T., Ito, M., Rasilo, M.-L., and Yamagata, T., Characterization of a major neutral glycolipid in PC12 cells as III3galα-globotriaosylceramide by the method for determing glycosphingolipid saccharide sequence with endoglycoceramidase. J. Biol. Chem., 263, 12124-12128 (1988) 9. Rasilo M.-L., and Yamagata, T., Occurrence of glucose polymer in undifferentiated PC12 cells. FEBS letters, 227, 197-194 (1988) 10. Rasilo M.-L., and Yamagata, T., Characterization of a glucose polymer from PC12 cells and neuronal cells of rat embryo. J. Biochem., 104, 742-754 (1988) 11. Ito, M., and Yamagata, T., Purification and characterization of glycosphingolipid-specific endoglycosidases (endoglycoceramidases) from a mutant strain of Rhodococcus sp. J. Biol. Chem., 264, 9510-9519 (1989) 12. Fukaya, N., Ito, M., Iwata, H., and Yamagata, T., Characterization of the glycosphingolipids of pig cortical bone and cartilage. Biochim. Biophys. Acta, 1004, 108-116 (1989) 13. Rasilo, M.-L., Ito, M., and Yamagata, T., Liberation of oligosaccharides from glycosphingolipids on PC12 cell surface with endoglycoceramidase. Biochem. Biophys. Res. Commun., 162, 1093-1099 (1989) 14. Rasilo, M.-L., and Yamagata, T., The glucose polymer of PC12 cells is susceptible to trypsinization. Biochem. Biophys. Res. Commun., 159, 1-6 (1989) 15. Higashi, H., Ito, M., Fukaya, N., Yamagata, S., and Yamagata, T., Two-dimensional mapping by the high-performance liquid chromatography of oligosaccharides released from glycosphingolipids by endoglycoceramidase. Anal. Biochem., 186, 355-362 (1990) 16. Kubo, T., Hayase, T., Morimoto, Y., Kondo, J., Matsumoto, R., Nagai, K., and Yamagata, T., Structural analysis of the carbohydrate chain of recombinant apolipoprotein E. Mitsubihsi Kasei R & D Review, 4, 1-7 (1990)(in Japanese) 17. Ito, M., Ikegami, Y., and Yamagata, T., Activator proteins for glycosphingolipid hydrolysis by endoglycoceramidases. J. Biol. Chem., 266, 7919-7926 (1991) 18. Shimamura, M., Oku, M., and Yamagata, T., The retarded rate of acid-catalyzed solvolysis of glycoside bonds between reducing- end glycose residue and ceramide in glycosphingolipids compared with that of glycoside bonds between hexopyranosides. FEBS letters, 290, 213-215 (1991) 19. Ito, M., Ikegami, Y., Omori, A., and Yamagata, T., Conversion of endoglycoceramidase-activator II by trypsin to the 27.9 kDa polypeptide possessing full activity: Purification of activator for endoglycoceramidase by trypsin treatment followed by trypsin-inhibitor agarose column application. J. Biochem. 110, 328-332 (1991) 20. Yamagata, T., and Yamazaki, K., Implanting mouse embryo stain with a LNF-I bearing fluorescent probe at their mural trophectodermal side. Biochem. Biophys. Res. Commun., 181, 1004-1009 (1991) 21. Higashi, H., Omori, A., and Yamagata, T. Calmodulin: A ganglioside-binding protein. J. Biol. Chem., 267, 9831-9838 (1992) 22. Higashi, H., and Yamagata, T., Mechanism for ganglioside-mediated modulation of calmodulin-dependent enzyme. J. Biol. Chem., 267, 9839-9843 (1992) 23. Shimamura, M., Oku, M., and Yamagata, T., Presence of murine fetal liver cells capable of being induced to differentiate in vitro into T cell receptor αβ positive cells. Eur. J. Immunol., 22, 2159-2164 (1992) 24. Shimamura, M., Oku, M., Ohta, S., and Yamagata, T., Hematopoietic cell lines capable of colonizing the thymus following in vivo transfer expressed T cell receptor δ gene immature mRNA. Immunol. 77, 369-376 (1992) 25. Shimamura, M., Oku, M., and Yamagata, T., A novel ganglioside expressed by mouse hematopoietic cell lines. J. Biol. Chem., 267, 18810-18813 (1992) 26. Higashi, H., and Yamagata, T., Full activation without calmodulin of calmodulin-dependent cyclic nucleotide phosphodiesterase by acidic glycosphingolipids; GM3, sialosylneolactotetraosylceramide and sulfatide. FEBS letters, 314, 53-57 (1992) 27. Ito, M., Ikegami, Y., and Yamagata, T., Kinetics of endoglycoceramidase action toward cell-surface glycosphingolipids of erythrocytes. Eur. J. Biochem., 218, 645-649 (1993) 28. Ito, M., Ikegami, Y., Tai, T., and Yamagata, T., Specific hydrolysis of intact erythrocyte cell-surface glycosphingolipids by endoglycoceramidase. Eur. J. Biochem., 218, 637-643 (1993) 29. Ponce, R.H., Yanagimachi, R., Urch, U.A., Yamagata, T., and Ito, M., Retention of hamster oolema fusibility with spermatozoa after various enzyme treatments: A search for the molecules involved in sperm-egg fusion. Zygotes, 1, 163-171 (1993) 30. Shimamura, M., Shibuya, N., Ito, M., and Yamagata, T., Repulsive contribution of surface sialic acid residues to cell adhesion to substratum. Biochem. Mol. Biol. Internatl., 33, 871-878 (1994) 31. Muramoto, K., Kawahara, M., Kobayashi, K., Ito, M., Yamagata, T., and Koroda, Y., Endoglycoceramidase treatment inhibits synchorous oscillations of intercellular Ca2+ in cultured cortical neuron. Biochem. Biophys. Res. Commun., 202, 398-402 (1994) 32. Li. J., Ito, M., Zhang, G., and Yamagata, T., The hydrolysis of cell surface glycoshingolipids by endoglycoceramidase reduces epidermal growth factor receptor phosphorylation in A431 cells. Glycobiology, 5, 343-350 (1995) 33. Nagatsuka, Y., Watari, S., Yasuda, T., Higashi, H., Yamagata, T., and Ono, Y., Production of human monoclonal antibodies to i blood group by EBV-induced transformation: Possible presence of a new glycolipid in cord blood cell membranes and human hematopoietic cell lines. Immunol. Lett., 46, 93-100 (1995) 34. Miura, R.O., Yamagata, S., Harada, T., Miura, Y., and Yamagata, T., Analysis of glycosaminoglycan degrading enzymes by substrate gel electrophoresis (Zymography). Anal. Biochem., 225, 333-340 (1995) 35. Miura, Y., Arai, T., and Yamagata, T., Synthesis of amphiphilic lactosides that possess a lactosylceramide-mimicking N-acyl structure. Carbohydrate Res., 289, 193-199 (1996) 36. Higashi, H., Yoshida, S., Sato, K., and Yamagata, T., Interaction of ganglioside and specific peptide sequences as a mechanism for modulation of calmodulin-dependent enzymes by gangliosides. J. Biochem., 120, 66-73 (1996) 37. Nagatsuka, Y., Yamaki, M., Watarai, S., Yasuda, T., Higashi, H., Yamagata, T., and Ono, Y., The Epstein-Barr virus (EBV) alters the B cell glycolipid which is recognized by the human monoclonal antibody to i-blood antigen. Virus Research, 43, 57-68 (1996) 38. Hyuga, S., Yamagata, S., Tai, T., and Yamagata, T., Inhibition of highly metastatic FBJ-LL cell migration by ganglioside GD1a highly expressed in poorly metastatic FBJ-S1 cells. Biochem. Biophys. Res. Commun., 231, 340-343 (1997) 39. Kikkawa, F., Tamakoshi, K., Nawa, A., Shibata, K., Yamagata, S., Yamagata, T., and Suganuma, N., Positive correlation between inhibitors of matrix metalloproteinase 1 and matrix metalloproteinases in malignant ovarian tumor tissues. J Cancer Lett., 120, 109-115 (1997) 40. Tamakoshi, K., Kikkawa, F., Yamagata, S., Yamagata, T., and Tomoda, Y., Hyaluronidase activity in gynecologic cancer tissue with different metastatic forms. British J. Cancer, 75, 1807-1811 (1997) 41. Suzuki, T., Horie, G., Yamazaki, Y., Kawabe, K., Masiuda, H., Miyamoto, D., Matsuda, M., Nishimura, S.,Yamagata, T., Ito, T., Kida, H., Kawaoka, Y., and Suzuki, T., Swine influenza virus strains recongnize sialylsugar chains containing the molecular species of sialic acid predominatly present in the swine tracheal epithelium. 42. Miura, Y., and Yamagata, T., Glycosylation of lactosylceramide analogs in animal cells; Amphipathic disaccharide primers for glycosphingolipid synthesis. 43. Podyma, K.A., Yamagata, S., Sakata, K., and Yamagata, T., Difference of hyaluronidase produced by human tumor cell lines with hyaluronidase present in human serum.as revealed by zymography Biochem. Biophys. Res. Commun., 241, 446-450 (1997) 44. Nakajima, H., Miura, Y., and Yamagata, T., Glycosylation of amphipathic lactoside primers with consequent inhibition of endogenous glysosphingolipid synthesis. J. Biochem., 124, 148-156 (1998) 45. Ji, L., Ito, M., Zhang, G., Hirabayashi, Y., Inokuchi, J., and Yamagata , T., Effects of endoglycoceramidase of D-threo-1-phenyl-2-decanoylamino-3-morpholino-1-propanol on glucose uptake, glycolysis, and mitochondrial respiration in HL60 cells. Arch Biochem. Biophys., 359, 107-14 (1998) 46. Miura, Y., Arai, T., Ohtake, A., Ito, M., Yamamoto, K., and Yamagata, T., Requirement for a different hydrophobic moiety and reliable chromogenic substrate for endo-type glycosylceramidases Glycobiology, 9, 957-960 (1999) 47. Nagaya, N., Yamagata, T., Yamagata, S., Iyoda, K., Ito, H., Hasegawa, Y., and Iwata, H., Examination of synovial fluid and serum hyaluronidase activity as a joint marker in rheumatoid arthritis and osteoarthritis patients (by zymography) Ann. Rheum. Dis., 58, 186-188 (1999) 48. Hyuga, S., Yamagata, S., Takatsu, Y., Hyuga, M., Nakanishi, H., Furukawa, K. and Yamagata, T., Suppression of FBJ-LL cell adhesion to vitronectin by ganglioside GD1a and loss of metastatic capacity. International J. Cancer, 83, 685-691 (1999) 49. Hyuga,S., Kawasaki, N., Hashimoto, O., Hyuga, M., Ohta, M., Yamagata, S., Yamagata, T., and Hayakawa, T., Possible role of hepatosyte growth gactor/scatter factor and activin A produced by the target organ in liver metastasis. Cancer Lett., 153, 137-143 (2000) 50 Kasuya, Maria C. Z., Wang, L. X., Lee, Y. C, Mitsuki, M., Nakajima, H.,Miura, Y., Sato, T., Hatanaka, K., Yamagata, S., and Yamagata, T., Azido glycoside primer: A versatile building block for the biocombinatorial synthesis of glycosphingolipid analogues. Carbohydr. Res. 329, 755-763 (2000) 51. Hyuga, S., Kawasaki, N., Ohta, M., Shibayama, R., Kawanishi, T., Yamagata, S., Yamagata, T., and Hayakawa, T., Ganglioside GD1a inhibits the HGF/SF-induced motility and scattering of cancer cells through suppression of tyrosine-phosphorylation of c-Met. International J. Cancer, 94, 328-334 (2001) 52. Nadanaka, S., Sato, C., Kitajima, K., Katagiri, K., Irie, S., and Yamagata, T., Occurrence of Oligosialic Acids on Integrin alpha5 Subunit and Their Involvement in Cell Adhesion to Fibronectin. J. Biol. Chem. 276, 33657-33664 (2001) [主要著作]（１９９０年以後） １. 糖鎖の検出 (電流測定法: アンペロメトリィ) 新生化學実験講座3, 糖質I, 糖タンパク質, (化學同人) 149-156 (1990) 2. エンドグリコセラミダーゼ 新生化學実験講座4, 脂質III, 糖脂質, (化學同人) 404-408 (1990) 3. 著床における糖鎖の役割 蛋白質?核酸?酵素, 37, 1783-1788 (1992) 4. 糖脂質糖鎖の細胞工學 糖鎖工學（産業調査會編）, 204-214 (1992) 5. 細胞質內にもある糖脂質 細胞, 25, 105-110 (1993) 6. 糖脂質の運命 グリコバイオロジ?シリ?ズ第２巻, (講談社), 179-191 (1993) 7. 細胞の恆常性維持と糖鎖 ”糖鎖”第Ｉ巻, 「糖鎖と生命」（永井克孝ほか編）, (東京化學同人), pp.78-85（1994） 8. ガングリオシドとカルモジュリンの結合とガングリオシドによるカルモジュリン依存性酵素の活性調節 ガングリオシド研究法２, (學會出版センター), pp.180-188 (1995) 9. ザイモグラフィーによるグリコサミノグリカン分解酵素の研究 週刊「醫學のあゆみ?細胞外マトリックス」、177、36-41、(1996) 10. ヒアルロニダーゼのザイモグラフィー(Zymography) [聯繫方式] Tatsuya Yamagata, D.Sc. Professor of Biochemistry, Cell Biology and Glycobiology Shenyang Pharmaceutical University P. O. Box 29 103 WenHua Road Shenyang 110016 P. R. China Business phone: [phone]-3005 Home phone: [phone] Cellular phone: [phone] E-mail: [email protected]（日文或英文） 主頁地址：http://niccou.51.net/

主要學術成果

代表性著作：１. 糖鎖の検出 (電流測定法: アンペロメトリィ) 新生化學実験講座3, 糖質I, 糖タンパク質, (化學同人) 149-156 (1990) 2. エンドグリコセラミダーゼ 新生化學実験講座4, 脂質III, 糖脂質, (化學同人) 404-408 (1990) 3. 著床における糖鎖の役割 蛋白質?核酸?酵素, 37, 1783-1788 (1992) 4. 糖脂質糖鎖の細胞工學 糖鎖工學（産業調査會編）, 204-214 (1992) 5. 細胞質內にもある糖脂質 細胞, 25, 105-110 (1993) 6. 糖脂質の運命 グリコバイオロジ?シリ?ズ第２巻, (講談社), 179-191 (1993) 7. 細胞の恆常性維持と糖鎖 ”糖鎖”第Ｉ巻, 「糖鎖と生命」（永井克孝ほか編）, (東京化學同人), pp.78-85（1994） 8. ガングリオシドとカルモジュリンの結合とガングリオシドによるカルモジュリン依存性酵素の活性調節 ガングリオシド研究法２, (學會出版センター), pp.180-188 (1995) 9. ザイモグラフィーによるグリコサミノグリカン分解酵素の研究 週刊「醫學のあゆみ?細胞外マトリックス」、177、36-41、(1996) 10. ヒアルロニダーゼのザイモグラフィー(Zymography)
代表性論文：1. Ito, M., and Yamagata, T., A novel glycosphingolipid-degrading enzyme cleaves the linkage between the oligosaccharide and ceramide of neutral and acidic glycosphingolipids. J. Biol. Chem., 261, 14278-14282 (1986) 2. Ito, M., Hirabayashi, Y., and Yamagata, T., Substrate specificity of endo-b-galactosidases from Flavobacterium keratolyticus and Escherichia freundii is different from that of Pseudomonas sp. J. Biochem., 100, 773-780 (1986) 3. Ohara, T., and Yamagata, T., Isolation and characterization of galactose-binding proteins from new-born mice. Biochim. Biophys. Acta, 884, 344-354 (1986) 4. Yamagata, S., and Yamagata, Y., The staining of type V collagen obtained from FBJ virus-induced osteosarcoma with concanavalin A. Biochem. Biophy. Res. Commun., 136, 857-862 (1986) 5. Higashi, H., Hirabayashi, Y., Ito, M., Yamagata, T., Matsumoto, M., Ueda, S., and Kato S., Immunostaining on thin-layer chromatograms of oligosaccharides released from gangliosides by endoglycoceramidase. J. Biochem., 102, 291-296 (1987) 6. Hirabayashi, J., Oda, Y., Ohara, T., Yamagata, T., and Kasai, K., Immunological cross-reactivity between β-galactoside-binding lectins of vertebrates. Possible relationship of antigenecity to oligomeric structure. Biochim. Biophys. Acta, 916, 321-327 (1987) 7. Mikawa, T., Sashida, R., Ito, M., and Yamagata, T., Rhodocuccus sp. producing endoglycoceramidase. Mitsubishi Chemical R & D Review, 1, 34-39 (1987)(in Japanese) 8. Shimamura, M., Hayase, T., Ito, M., Rasilo, M.-L., and Yamagata, T., Characterization of a major neutral glycolipid in PC12 cells as III3galα-globotriaosylceramide by the method for determing glycosphingolipid saccharide sequence with endoglycoceramidase. J. Biol. Chem., 263, 12124-12128 (1988) 9. Rasilo M.-L., and Yamagata, T., Occurrence of glucose polymer in undifferentiated PC12 cells. FEBS letters, 227, 197-194 (1988) 10. Rasilo M.-L., and Yamagata, T., Characterization of a glucose polymer from PC12 cells and neuronal cells of rat embryo. J. Biochem., 104, 742-754 (1988) 11. Ito, M., and Yamagata, T., Purification and characterization of glycosphingolipid-specific endoglycosidases (endoglycoceramidases) from a mutant strain of Rhodococcus sp. J. Biol. Chem., 264, 9510-9519 (1989) 12. Fukaya, N., Ito, M., Iwata, H., and Yamagata, T., Characterization of the glycosphingolipids of pig cortical bone and cartilage. Biochim. Biophys. Acta, 1004, 108-116 (1989) 13. Rasilo, M.-L., Ito, M., and Yamagata, T., Liberation of oligosaccharides from glycosphingolipids on PC12 cell surface with endoglycoceramidase. Biochem. Biophys. Res. Commun., 162, 1093-1099 (1989) 14. Rasilo, M.-L., and Yamagata, T., The glucose polymer of PC12 cells is susceptible to trypsinization. Biochem. Biophys. Res. Commun., 159, 1-6 (1989) 15. Higashi, H., Ito, M., Fukaya, N., Yamagata, S., and Yamagata, T., Two-dimensional mapping by the high-performance liquid chromatography of oligosaccharides released from glycosphingolipids by endoglycoceramidase. Anal. Biochem., 186, 355-362 (1990) 16. Kubo, T., Hayase, T., Morimoto, Y., Kondo, J., Matsumoto, R., Nagai, K., and Yamagata, T., Structural analysis of the carbohydrate chain of recombinant apolipoprotein E. Mitsubihsi Kasei R & D Review, 4, 1-7 (1990)(in Japanese) 17. Ito, M., Ikegami, Y., and Yamagata, T., Activator proteins for glycosphingolipid hydrolysis by endoglycoceramidases. J. Biol. Chem., 266, 7919-7926 (1991) 18. Shimamura, M., Oku, M., and Yamagata, T., The retarded rate of acid-catalyzed solvolysis of glycoside bonds between reducing- end glycose residue and ceramide in glycosphingolipids compared with that of glycoside bonds between hexopyranosides. FEBS letters, 290, 213-215 (1991) 19. Ito, M., Ikegami, Y., Omori, A., and Yamagata, T., Conversion of endoglycoceramidase-activator II by trypsin to the 27.9 kDa polypeptide possessing full activity: Purification of activator for endoglycoceramidase by trypsin treatment followed by trypsin-inhibitor agarose column application. J. Biochem. 110, 328-332 (1991) 20. Yamagata, T., and Yamazaki, K., Implanting mouse embryo stain with a LNF-I bearing fluorescent probe at their mural trophectodermal side. Biochem. Biophys. Res. Commun., 181, 1004-1009 (1991) 21. Higashi, H., Omori, A., and Yamagata, T. Calmodulin: A ganglioside-binding protein. J. Biol. Chem., 267, 9831-9838 (1992) 22. Higashi, H., and Yamagata, T., Mechanism for ganglioside-mediated modulation of calmodulin-dependent enzyme. J. Biol. Chem., 267, 9839-9843 (1992) 23. Shimamura, M., Oku, M., and Yamagata, T., Presence of murine fetal liver cells capable of being induced to differentiate in vitro into T cell receptor αβ positive cells. Eur. J. Immunol., 22, 2159-2164 (1992) 24. Shimamura, M., Oku, M., Ohta, S., and Yamagata, T., Hematopoietic cell lines capable of colonizing the thymus following in vivo transfer expressed T cell receptor δ gene immature mRNA. Immunol. 77, 369-376 (1992) 25. Shimamura, M., Oku, M., and Yamagata, T., A novel ganglioside expressed by mouse hematopoietic cell lines. J. Biol. Chem., 267, 18810-18813 (1992) 26. Higashi, H., and Yamagata, T., Full activation without calmodulin of calmodulin-dependent cyclic nucleotide phosphodiesterase by acidic glycosphingolipids; GM3, sialosylneolactotetraosylceramide and sulfatide. FEBS letters, 314, 53-57 (1992) 27. Ito, M., Ikegami, Y., and Yamagata, T., Kinetics of endoglycoceramidase action toward cell-surface glycosphingolipids of erythrocytes. Eur. J. Biochem., 218, 645-649 (1993) 28. Ito, M., Ikegami, Y., Tai, T., and Yamagata, T., Specific hydrolysis of intact erythrocyte cell-surface glycosphingolipids by endoglycoceramidase. Eur. J. Biochem., 218, 637-643 (1993) 29. Ponce, R.H., Yanagimachi, R., Urch, U.A., Yamagata, T., and Ito, M., Retention of hamster oolema fusibility with spermatozoa after various enzyme treatments: A search for the molecules involved in sperm-egg fusion. Zygotes, 1, 163-171 (1993) 30. Shimamura, M., Shibuya, N., Ito, M., and Yamagata, T., Repulsive contribution of surface sialic acid residues to cell adhesion to substratum. Biochem. Mol. Biol. Internatl., 33, 871-878 (1994) 31. Muramoto, K., Kawahara, M., Kobayashi, K., Ito, M., Yamagata, T., and Koroda, Y., Endoglycoceramidase treatment inhibits synchorous oscillations of intercellular Ca2+ in cultured cortical neuron. Biochem. Biophys. Res. Commun., 202, 398-402 (1994) 32. Li. J., Ito, M., Zhang, G., and Yamagata, T., The hydrolysis of cell surface glycoshingolipids by endoglycoceramidase reduces epidermal growth factor receptor phosphorylation in A431 cells. Glycobiology, 5, 343-350 (1995) 33. Nagatsuka, Y., Watari, S., Yasuda, T., Higashi, H., Yamagata, T., and Ono, Y., Production of human monoclonal antibodies to i blood group by EBV-induced transformation: Possible presence of a new glycolipid in cord blood cell membranes and human hematopoietic cell lines. Immunol. Lett., 46, 93-100 (1995) 34. Miura, R.O., Yamagata, S., Harada, T., Miura, Y., and Yamagata, T., Analysis of glycosaminoglycan degrading enzymes by substrate gel electrophoresis (Zymography). Anal. Biochem., 225, 333-340 (1995) 35. Miura, Y., Arai, T., and Yamagata, T., Synthesis of amphiphilic lactosides that possess a lactosylceramide-mimicking N-acyl structure. Carbohydrate Res., 289, 193-199 (1996) 36. Higashi, H., Yoshida, S., Sato, K., and Yamagata, T., Interaction of ganglioside and specific peptide sequences as a mechanism for modulation of calmodulin-dependent enzymes by gangliosides. J. Biochem., 120, 66-73 (1996) 37. Nagatsuka, Y., Yamaki, M., Watarai, S., Yasuda, T., Higashi, H., Yamagata, T., and Ono, Y., The Epstein-Barr virus (EBV) alters the B cell glycolipid which is recognized by the human monoclonal antibody to i-blood antigen. Virus Research, 43, 57-68 (1996) 38. Hyuga, S., Yamagata, S., Tai, T., and Yamagata, T., Inhibition of highly metastatic FBJ-LL cell migration by ganglioside GD1a highly expressed in poorly metastatic FBJ-S1 cells. Biochem. Biophys. Res. Commun., 231, 340-343 (1997) 39. Kikkawa, F., Tamakoshi, K., Nawa, A., Shibata, K., Yamagata, S., Yamagata, T., and Suganuma, N., Positive correlation between inhibitors of matrix metalloproteinase 1 and matrix metalloproteinases in malignant ovarian tumor tissues. J Cancer Lett., 120, 109-115 (1997) 40. Tamakoshi, K., Kikkawa, F., Yamagata, S., Yamagata, T., and Tomoda, Y., Hyaluronidase activity in gynecologic cancer tissue with different metastatic forms. British J. Cancer, 75, 1807-1811 (1997) 41. Suzuki, T., Horie, G., Yamazaki, Y., Kawabe, K., Masiuda, H., Miyamoto, D., Matsuda, M., Nishimura, S.,Yamagata, T., Ito, T., Kida, H., Kawaoka, Y., and Suzuki, T., Swine influenza virus strains recongnize sialylsugar chains containing the molecular species of sialic acid predominatly present in the swine tracheal epithelium. 42. Miura, Y., and Yamagata, T., Glycosylation of lactosylceramide analogs in animal cells; Amphipathic disaccharide primers for glycosphingolipid synthesis. 43. Podyma, K.A., Yamagata, S., Sakata, K., and Yamagata, T., Difference of hyaluronidase produced by human tumor cell lines with hyaluronidase present in human serum.as revealed by zymography Biochem. Biophys. Res. Commun., 241, 446-450 (1997) 44. Nakajima, H., Miura, Y., and Yamagata, T., Glycosylation of amphipathic lactoside primers with consequent inhibition of endogenous glysosphingolipid synthesis. J. Biochem., 124, 148-156 (1998) 45. Ji, L., Ito, M., Zhang, G., Hirabayashi, Y., Inokuchi, J., and Yamagata , T., Effects of endoglycoceramidase of D-threo-1-phenyl-2-decanoylamino-3-morpholino-1-propanol on glucose uptake, glycolysis, and mitochondrial respiration in HL60 cells. Arch Biochem. Biophys., 359, 107-14 (1998) 46. Miura, Y., Arai, T., Ohtake, A., Ito, M., Yamamoto, K., and Yamagata, T., Requirement for a different hydrophobic moiety and reliable chromogenic substrate for endo-type glycosylceramidases Glycobiology, 9, 957-960 (1999) 47. Nagaya, N., Yamagata, T., Yamagata, S., Iyoda, K., Ito, H., Hasegawa, Y., and Iwata, H., Examination of synovial fluid and serum hyaluronidase activity as a joint marker in rheumatoid arthritis and osteoarthritis patients (by zymography) Ann. Rheum. Dis., 58, 186-188 (1999) 48. Hyuga, S., Yamagata, S., Takatsu, Y., Hyuga, M., Nakanishi, H., Furukawa, K. and Yamagata, T., Suppression of FBJ-LL cell adhesion to vitronectin by ganglioside GD1a and loss of metastatic capacity. International J. Cancer, 83, 685-691 (1999) 49. Hyuga,S., Kawasaki, N., Hashimoto, O., Hyuga, M., Ohta, M., Yamagata, S., Yamagata, T., and Hayakawa, T., Possible role of hepatosyte growth gactor/scatter factor and activin A produced by the target organ in liver metastasis. Cancer Lett., 153, 137-143 (2000) 50 Kasuya, Maria C. Z., Wang, L. X., Lee, Y. C, Mitsuki, M., Nakajima, H.,Miura, Y., Sato, T., Hatanaka, K., Yamagata, S., and Yamagata, T., Azido glycoside primer: A versatile building block for the biocombinatorial synthesis of glycosphingolipid analogues. Carbohydr. Res. 329, 755-763 (2000) 51. Hyuga, S., Kawasaki, N., Ohta, M., Shibayama, R., Kawanishi, T., Yamagata, S., Yamagata, T., and Hayakawa, T., Ganglioside GD1a inhibits the HGF/SF-induced motility and scattering of cancer cells through suppression of tyrosine-phosphorylation of c-Met. International J. Cancer, 94, 328-334 (2001) 52. Nadanaka, S., Sato, C., Kitajima, K., Katagiri, K., Irie, S., and Yamagata, T., Occurrence of Oligosialic Acids on Integrin alpha5 Subunit and Their Involvement in Cell Adhesion to Fibronectin. J. Biol. Chem. 276, 33657-33664 (2001)