![王丹[重慶大學副教授]](/img/b/46b/nBnauM3XwQDN4UDMwIzN5IDN0UTMyITNykTO0EDMwAjMwUzLyczL0IzLt92YucmbvRWdo5Cd0FmLzE2LvoDc0RHa.jpg "王丹[重慶大學副教授]")
研究方向
生物催化和代謝工程：主要開展以具有生物活性的酶和全細胞作為催化劑，以可再生生物質為原料的生物催化研究；以生物轉化的過程最佳化為主要研究對象。此外，還開展從頭設計的代謝途徑研究，計算機模擬技術和濕實驗相結合構建細胞工廠。
天然產物和蛋白分離純化：利用膜分離、吸附及離子交換、親和色譜等技術，開展天然產物、基因工程藥物、酶等的分離工藝及作用機制研究，實現多種生物活性物質的分離純化如，白藜蘆醇，琥珀酸，蛋白A，重組人粒細胞集落刺激因子，重組人上表皮生長因子等的分離純化。
學術成就
主持研究國家自然科學基金1項，重慶市自然科學基金2項，南京工業大學材料工程國家重點實驗室開放基金1項。作為主要研究人員參與國家973，863及中科院知識創新工程，在本領域重點期刊包括Enzyme and Microbial Technology,Biotechnology and Bioengineering, BioresourceTechnology,BiochemicalJournal,Applied and MicrobiolBiotechnology等發表論文30餘篇，其中被SCI收錄30篇，發表會議論文8篇，他引245次，申請發明專利5項，美國專利兩項，獲授權發明專利兩項。是國際期刊Enzymeand MicrobialTechnology,Applied and Mic -robiolBiotechnology, Bioresources等雜誌審稿人，國家自然科學基金函評專家。2017年6月12日，王丹副教授科研團隊發表在Bioresource Technology期刊（SCI 1區，影響因子5.651）上的研究論文The enhancement of the hydrolysis of bamboo biomass in ionic liquid with chitosan-based solid acid catalysts immobilized with metal ions被“可再生能源全球創新”網站（Renewable Energy Global Innovations）作為Bioenergy進行了特別報導。
科研項目
主持重慶市自然科學基金：先進燃料生物基脂肪酸及其衍生物的可控合成研究
主持中央高校基本業務費：基於微納結構殼聚糖微球的合成研究
主持材料化學工程國家重點實驗室開放基金：基於微納結構殼聚糖微球的合成研究
參與中國國家高技術研究發展計畫（863計畫）（NO.2011AA02A203和2012AA022301）2012-2015
參與中國科學院知識創新工程重要方向項目
主持國家自然科學基金青年基金：利用模組化間隔序列調控人工操縱子構建細胞工廠產琥珀酸的研究
主持重慶市自然科學基金：接枝賴氨酸柔性臂的交聯殼聚糖微球固定化酶系統的研究及套用
主持重慶大學優秀青年教師人才引進項目
代表論文
[1]Le Mi, Dandan, Jie Cheng, Dan Wang*, Sa Li, Xuetuan Wei. 2017. Efficient production of free fatty acids from ionic liquid-based acid- or enzyme-catalyzed bamboo hydrolysate. J. Ind. Microbiol. Biotechnol. 44, 419-430. (*通訊作者，IF=2.81)
[2] Dandan Qin, Yuanliang Hu, Jie Cheng, Nan Wang, Sha Li, Dan Wang*. Au auto-inducibleEscherichia coli strain obtained by adaptively laboratory evolution for fatty acid synthesis from ionic liquid-treated bamboo hydrolystae.Bioresour. Technol. , 2016, Sep. 9.(*通訊作者，IF=4.96)
[3] Jie Cheng, Nan Wang, Dezhou Zhao, Dandan Qin, Wenqing Si, Yunfei Tan, Shun’an Wei, Dan Wang*. The enhancement of the hydrolysis of bamboo biomass in ionic liquid with chitosan-based solid acid catalysts immobilized with metal ions.Bioresour. Technol. , 2016, Aut. 24. (*通訊作者，IF=4.96)
[4] Wen Jiang, Zhiming Zhou, Dan Wang*, Xiaohua Zhou*, Renyou Tao, Yang Yang, Yexin Shi, Guiluo Zhang, Deyi Wang, Zhen Zhou*. Transglutaminase catalyzed hydrolyzed wheat gliadin grafted with chitosan oligosaccharide and its characterization.Carbohydr. Polym., 2016, Jul. 21. doi:(*通訊作者，IF=3.479)
[5] Dan Wang, Ka-Yiu San*. New pathway to produce hydroxylfatty acid without heterologous expression of hydroxylase.Metab.Eng
[6] Dan Wang, Chandresh Thakker, Ping Liu, George N. Benett, Ka-Yiu San*. Efficient production of free fatty acids from soybeanmeal carbohydrate.Biotechnol.Bioeng. , 2015,May 5
[7] Dan Wang, Hui Wu, Chandresh Thakker, Jared Beyersdorf, George N. Benett, Ka-Yiu San*.Efficient free fatty acid production in engineeredEscherichia colistrains using soybean oligosaccharides as feedstock.Biotech.Prog.
[8] Jing Wang, Dandan Qin, Baoyun Zhang, Qiang Li, Lichun Dong, Xiaohua Zhou, Dan Wang*.Fine-tuning of ecaA and pepc gene expression increases succinic acid production inEscherichia coli.Appl. Micorbiol. Biotechnol.
[9] Nan Wang, shun’an Wei, Dan Wang*. Chitosan-based solid acid for hydrolysis of bamboo biomass in 1-butyl-3-methylimidazolium chloride.Green Chem.
[10] Jing Wang, Baoyun Zhang, Jie Zhang, Honghui Wang, Minghui Zhao, Nan Wang, Lichun Dong, Xiaohua Zhou, Dan Wang*. Enhanced succinic acid production and magnesium utilization by overexpression of magnesium transportmgtAinEscherichia coli.Bioresour. Technol.
[11] Nan Wang, Jie Zhang,HonghuiWang,Qiang Li,Sun’anWei, Dan Wang*. Effects of metal ions on the hydrolysis of bamboo biomass in 1-butyl-3-methylimidazolium chloride with dilute acid as catalyst.Bioresour. Technol.
[12] Jie Zhang, Xiaohua Zhou, Dan Wang*, Xing Zhou, Fang Yun, Shiyu Tan. A comprehensive study of extraction of L-lysine with sec-octylphenoxy acetic acid in sulfonated kerosene.Sep. Sci. Technol.
[13] Jie Zhang, Dan Wang*, Jiachuan Pan, Jing Wang, Hongbo Zhao, Qiang Li, Xiaohua Zhou. Efficient resveratrol production by immobilized β-glucosidase on cross-linked chitosan microsphere modified by L-lysine.J. Mol. Catal. B-Enzym.
[14] Wang Honghui, Jiachuan Pan, Dan Wang*, Jing Wang, Nan Wang, Lin Xu, Qiang Li, Xiaohua Zhou.Succinic acid production from xylose mother liquor by recombinantEscherichia colistrain .Biotechnol.Biotec.eq.
[15] Jing Wang, Qiang Li, Xiaohua Zhou, Dan Wang*. Enhanced succinic acid production and magnesium utilization by overexpression of magnesium transportMgtAinEscherichia coli. 2014, ASM general meeting, Boston.
[16] Xuetuan Wei,Yinhua Zhou,Jingbang Chen,Dongbo Cai, Dan Wang,Gaofu Qi,Shouwen Chen. Efficient expression of nattokinase inBacillus licheniformis: host strain construction and signal peptide optimization. J Ind Microbiol Biot, 2014
[17] Jing Wang, Honghui Wang, Nan Wang, Qiang Li, Dan Wang*. Combinatorial engineering of intergenic regions in operons to improve the succinic acid production inEscherichia coli. 2014 Fall Meeting, ASM Branches
[18] 王丹, 王洪輝, 王競, 汪楠, 張傑, 邢建民.糠醛和5-羥甲基糠醛對大腸桿菌產丁二酸的影響.中國生物工程學報
[19] Jie Zhang, Xiaohua Zhou*, Dan Wang*, Luyan Sang, Yuanhong peng, Shiyu Tan, Qiang Li. High selective separation of flavonoids based on crosslinked chitosan resin grafted by quercetin.Sep. Sci. Technol.
[20] Jie Zhang, Xiaohua Zhou *, Dan Wang*, Yingli Wang, Xing Zhou, Honghui Wang, Qiang Li, Shiyu Tan. Studies on the co-immobilized GOD/CAT on cross-linked chitosan microsphere modified by lysine.J. of Mol. Catal. B: Enzym.
[21] Jie Zhang, Xiaohua Zhou*, Zhen Zhou, Yu Su, Ruiya Zuo, DanWang*, Shiyu Tan. Preparation and characterization of a novel Ala-Gln dipeptide-Zncomplex.AsianJ.Chem.
[22] Jie Zhang, Xiaohua Zhou, DanWang*, YonghongWang, LinChen,Shiyu Tan.Selective separation of L-aromatic amino acid by L-phenylalanine-crosslinked chitosan resin.AsianJ.Chem.
[23] Qiang Li, Jiandu Lei, Rongyue Zhang, Juan Li, Jianmin Xing, Fei Gao, Fangling Gong, Xaofeng Yan, Dan Wang, Zhiguo Su, Guanghui Ma. Efficient decolorization and deproteinization using uniform polymer microspheres in the succinic acid biorefinery from bio-waste cotton (Gossypium hirsutumL.) stalks.Bioresour. Technol.
[24] Jie Zhang, Xiaohua Zhou, H Xu, Luyan Sang, DanWang*. Selective Adsorption of Flavonoids on Chitosan Resin Grafted by Quercetin.Proc. - Int. Conf. Biomed. Eng. Biotechnol.
[25] Ziyu Song, Qiang Li, Dan Wang, Jie Zhang, and Jianmin Xing. A novel up-flow inner-cycle anoxic bioreactor (UIAB) system for the treatment of sulfide wastewater from purification of biogas.Water Sci. Technol.
[26] Dan Wang, Qiang Li, Ziyu Song, Wei Zhou, Zhiguo Su, Jianmin Xing. High cell density fermentation via a metabolically engineeredEscherichia colifor the enhanced production of succinic acid .J.Chem.Technol .Biotechnol.
[27] Qiang Li *, Dan Wang*, Guangyu Hu, Jianmin Xing, Zhiguo Su. Integrated bioprocess for high-efficiency production of succinic acid in an expanded-bed adsoption system.Biochem. Eng. J.
[28] ZelongWang, DanWang, QiangLi, WangliangLi, HuangTang and JianminXing.Enhanced biodesulfurization by expression of dibenzothiophene uptake genes inRhodococcus erythropolis.World J. Microb. Biot.
[29] Dan Wang, Qiang Li, Yu Mao,Jianmin Xing, Zhiguo Su.High-level succinic acid production and yield by lactose-induced expression of phosphoenolpyruvate carboxylase inptsGmutantEscherichia coli.Appl.Microbiol. Biotechnol.
[30] Qiang Li, Dan Wang, Ziyu Song, Wei Zhou, Yong Wu, Jianmin Xing, Zhiguo Su.Dual-phase fermentation enables Actinobacillus succinogenes 130ZT to be a potential role for high-level lactate production from the bioresource.Bioresource Technol.
[31] Qiang Li, Dan Wang, Yunjian Li, Yunjian Zhang, Jianmin Xing, Zhiguo Su. One step recovery of succinic acid from fermentation broths by crystallization.Sep.Purif.Technol.
[32] Qiang Li, Dan Wang, Jianmin Xing, Zhiguo Su.Kinetic evaluation of products inhibition to succinic acid producers Escherichia coli NZN111, AFP111 BL21 and Actinobacillus succinogenes 130ZT.J.Microbiol.
[33] Qiang Li, Maohua Yang, Dan Wang, Wangliang Li, Yong Wu, Yunjian Zhang, Jianmin Xing, Zhiguo Su. Efficient conversion of crop stalk wastes into succinic acid production byActinobacillus succinogenes.Bioresour. Technol.
[34] QiangLi, WangliangLi, DanWang, BinbinLiu, HuangTang, MaohuaYang, JianminXing, ZhiguoSu. pH Neutralization while Succinic Acid Adsorption ontoAnion-Exchange Resins.Appl. Biochem. Biotechnol.
[35] Dan Wang, Qiang Li,Wangliang Li,Jianmin Xing, Zhiguo Su. Improvement of succinate production by overexpression of a cyanobacterial carbonic anhydrase inEscherichia coli.Enzyme Microbial. Technol.
[36] Wangliang Li, Huang Tang, Qingfen Liu, Jianmin Xing, Qiang Li, DanWang, Maohua Yang, Xin Li, Huizhou Liu. Deep desulfurization of diesel by integrating adsorption and microbial method.Biochem.Eng.J.
[37] Dan Wang, Qiang Li,Wangliang Li,Jianmin Xing, Zhiguo Su. Overexpression of a cyanobacterial carbonic anhydrase inEscherichia colienhances succinic acid production.J. Biotechnol.
[38] D.Wang, Q.Li, Z.Su, J.Xing. Effects of coexpression of carbonic anhydrase and phosphoenolpyruvate carboxylase on succinic acid production inEscherichia coli. The 3rd International Symposium on Advance Biological Engineering and Scienc.
[39] D.Wang, Z.Su, J.Xing. Efficient production of succinic acid from corn stalk hydrolysates by a recombinantEscherichia coliwithptsGmutation. The 2010 International Conference on Biomass Energy and Technologies.
[40] D.Wang, Q.Li, Z.Song, W.Zhou, J.Xing, Z.Su.High cell density fermentation of a metabolically engineeredEscherichia coliin fed-batch cultivation for the production of succinic acid. The 2010 International Conference on Biomass Energy and Technologies.
[41] D.Wang, Q.Li, W.Li, M.Yang, J.Xing, Z.Su. Effects of carbonic anhydrase onsuccinic acid production inEscherichia coli. The 2nd International Symposium on Advance Biological Engineering and Scienc.
[42] D.Wang, Q.Li, W.Li, Q.Liu, J.Xing, Z.Su. Overexpression of a cyanobacterial carbonic anhydrase inEscherichia colienhances succinic acid production. The 13th International Biotechnology Symposium & Exhibition.
[43] D.Wang, J.Wang, Q.Li, W.Li, J.Xing, Z.Su. Enhanced succinate production by lactose-induced expression of phosphoenolpyruvate carboxylase in △ptsGmutantE. coli. The 5th sino-US Joint Conference of Chemical Engineering, 2009, 10.13-10.16, Beijing, China.
[44] 王丹, 毛雨, 馬蘭, 李強, 李望良, 邢建民, 蘇志國. 乳糖誘導丙酮酸羧化酶基因在大腸桿菌中的表達及對琥珀酸產量的影響.生物工程學報
[45] 馬婕, 王丹, 李強, 邢建民, 劉會洲. 基因工程大腸桿菌合成γ-聚谷氨酸.過程工程學報
[46] 張玉秀，王姣， 王丹，齊峰. 大腸桿菌產琥珀酸基因工程研究進展.中國生物工程雜誌
[47] Dexi Wu, Qiang Li, Dan Wang, Yugang Dong. Enzymatic hydrolysis and succinic acid fermentation from steam-exploded corn stalk at high solid concentration by recombinantEscherichia coli.Appl. Biochem. Biotechnol.
[48] Dan Wang, Qiang Li, Maohua Yang, Yunjian Zhang, Zhiguo Su, Jianmin Xing. Efficient production of succinic acid from corn stalk hydrolysates by a recombinantEscherichia coliwith ptsG mutation.Process Biochem.
[49] 毛雨， 王丹, 黃占斌, 邢建民. 微生物原生質體融合育種技術及其套用.中國生物工程雜誌
[50] 毛雨， 王丹，李強，邢建民，黃占斌. 產琥珀酸放線桿菌的原生質體製備與再生.中國生物工程雜誌
專利
[1] 王丹, 王競, 王洪輝, 汪楠, 周小華. 生產丁二酸的重組質粒、基因工程菌的構建及用途. 2013，中國.(第一發明人）
[2] 王丹, 王洪輝, 汪楠, 王競, 周小華. 木糖醇結晶母液製備葡萄糖酸的反應分離耦合裝置和工藝. 2013，中國.(第一發明人）
[3] 王丹, 汪楠, 魏順安, 王洪輝, 王競, 張傑, 周小華. 一種降解木質纖維素生成還原糖的方法. 2013，中國.(第一發明人）
[4] 王丹, 周茂祥, 李茹, 蔡錦華, 顏李秀, 周小華. 一種製備多孔交聯殼聚糖微球的新方法. 2013，中國. (第一發明人）
[5] 王丹, 黃小栩, 盧浩, 王孟, 周小華. 一種處理木糖母液的方法. 2011，中國.(第一發明人）
[6]李強, 邢建民, 王丹, 宋子煜, 張雲劍. 一種利用離子交換樹脂對丁二酸發酵液進行脫色的方法. 2011，中國.(第三發明人）
[7]李強, 邢建民, 王丹, 宋子煜, 張雲劍. 一種連續結晶生物製造丁二酸的裝置及方法.2011，中國. (第三發明人）
[8] 邢建民，周威，李強，李望良，宋子煜， 王丹. 一種具有硫酸鹽還原功能的檸檬酸桿菌及其套用. 2011，中國.
