林金萍

"Liu

女,工學博士,副研究員。
華東理工大學生物化學與分子生物學專業:碩士生導師。

主要研究方向

1.酶的篩選、發酵、純化和功能表征;
2.酶的結構和功能解析
3.生物催化合成手性化合物和生物基產品

目前承擔和參加的科研項目

1.國家自然科學基金“基於醛代謝阻斷的乙醇醛的高效生物合成研究”(21002029/B020706).
2.973子課題“微生物藥物合成相關的若干重要酶蛋白的構效關係與進化” (2012CB721003)
3.國家自然科學基金“氧化葡萄糖酸桿菌NAD(P)H氧化酶基因的活性鑑定及其功能和表達調控的研究”(20976053/B060804).
4.863重大項目“化工有機酸的生物轉化技術”(No.2011AA02A213)

近期發表的論文

1. Xu Liu, Zuanning Yuan, Y. Adam Yuan, Jinping Lin*, Dongzhi Wei. Biochemical and structural analysis of Gox2181, a new member of the SDR superfamily from Gluconobacter oxydans. Biochemical and Biophysical Research Communications. 2011,415: 410-415
2. Jian Wu, Minhua Li, Jinping Lin*, Dongzhi Wei. Highly Selective Oxidation of Benzyl Alcohol Using Engineered Gluconobacter Oxydans in Biphasic System. Current Microbiology. 2011, 62:1123-1127
3. Jian Wu, Minghua Li, Jinping Lin*, Dongzhi Wei*.Determination of Dihydroxyacetone and Glycerol in Fermentation Process by GC after n-Methylimidazole Catalyzed Acetylation. Journal of Chromatographic Science. 2011, 49(5): 375-378
4. Jian Wu, Jiale Wang, Minhua Li, Jinping Lin*, Dongzhi Wei**. Optimization of immobilization for selective oxidation of benzyl alcohol by Gluconobacter oxydans using response surface methodology. Bioresource Technology. 2010, 101:8936-8941
5. Minghua Li, Jian Wu, Xu Liu, Jinping Lin*, Dongzhi Wei**, Hao Chen. Enchanced production of dihydroxyacetone from glycerol by overexpression of glycerol dehydrogenase in an alcohol dehydrogenase-deficient mutant of Gluconobacter oxydans. Bioresource Technology. 2010, 101:8294-8299
6. Minghua Li, Jian Wu, Jinping Lin*, Dongzhi Wei. Expression of Vitreoscilla Hemoglobin Enhances Cell Growth and Dihydroxyacetone Production in Gluconobacter oxydans. Current Microbiology. 2010,61:370-375
7. Minmin Chen, Jinping Lin*, Yushu Ma, Dongzhi Wei. Characterization of a Novel NADPH-Dependent Oxidoreductase From Gluconobacter oxydans. Molecular biotechnology. 2010, 46:176-181
8. Lin Zhang, Jinping Lin*, Yushu Ma, Dongzhi Wei, Ming Sun.Construction of a novel shuttle vector for use in Gluconobacter oxydans. Molecular biotechnology. 2010, 46:227-233

專利

1. 林金萍,魏東芝,吳建。一種同時分析微生物發酵液中甘油和二羥基丙酮的方法。公開號:CN101566606A,授權公告號:CN 101566606 B
2.魏東芝,林金萍,魏國棟。一種套用靜息細胞製備光學純D-型β-羥基酸的方法。公開號:CN101280325A
3.魏東芝,林金萍,高瓊。一種微生物不對稱拆分製備(S)-苯乙二醇的方法。公開號:CN 101302543A
4.魏東芝,林金萍,許偉欽。從頭孢克洛萘酚複合物中提純頭孢克洛的方法。公開號:CN 101314605
5.魏東芝,林金萍,張敏潔。一種酶法連續生產聚(ε-己內酯)的方法。公開號:CN101781398A

獲獎

工業酶製劑的開發與套用關鍵技術.上海市科技進步一等獎. 2010

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