個人簡介
吳長艾，女，山東巨野人。2004年獲得山東農業大學理學博士學位，至今在山東農業大學生命科學學院工作。生物化學與分子生物學國家級教學團隊成員。承擔本科生《生物化學A》、《基礎生物化學B》和《基因工程》；研究生《基因工程原理和技術》等課程的教學工作。“泰山學者”特聘崗位學術骨幹。主要從事重要經濟作物棉花及模式植物擬南芥耐鹽、抗旱等重要基因的克隆、鑑定，探討其調控機制及在作物遺傳改良上的套用價值。近年來，承擔了轉基因生物新品種培育科技重大專項、國家自然科學基金和山東省自然基金等課題6項，參加2項。獲國家發明專利4項。在《Plant and cell Physiology》、《RNA》、《FEBS Journal》、《Plant Science》和《作物學報》等國內外著名學術刊物上發表研究論文30餘篇。
教學工作
承擔本科生《基礎生物化學》，《基礎生物化學實驗》；研究生 《基因工程原理與技術》、《基因表達調控》等課程的教學工作。
研究方向
1. 棉花和擬南芥鹽鹼、乾旱等非生物逆境回響基因的分離、鑑定及分子調控
2. 棉花和擬南芥鹽鹼、乾旱等非生物逆境回響的脫落酸、茉莉酸等激素調控
科研項目
1. 轉基因生物新品種培育科技重大專項《棉花抗旱、耐鹽和低溫重要新基因的克隆和功能驗證》2009-2012，主持
2. 國家自然科學基金《棉花鹽誘導的4個海藻糖6-磷酸合酶和6個LEA基因的生理功能及其套用研究》2011-2013，主持
3. 國家自然科學基金《五個擬南芥鹽和乾旱誘導的miRNAs的功能鑑定及調控途徑研究》2010-2012，主持
4. 國家自然科學基金《水稻乾旱、鹽脅迫相關microRNAs的分離和表達調控分析》2006-2008，主持
5. 教育部“長江學者創新團隊發展計畫”《作物高產優質的生理與分子基礎》2007-2009，子課題主持
6. 山東省自然科學基金《“棉花新型轉錄調節因子GhPLATZ1的功能和作用機理研究》，2015-2017，主持
7. 山東農業大學博士基金《棉花Na+/H+逆向運轉體基因的耐鹽機理及其轉基因耐鹽棉花新種質的創造》2005-2007，主持
8. 國家自然科學基金《棉花GhSAG基因的功能分析和分子機理研究》， 2015-2018，第二位
9. 973前期《玉米高溫乾旱抗性關鍵 microRNA基因的分離及功能鑑定》，2014-2016，第二位
個人榮譽
1. 2012年獲得山東農業大學“青年崗位能手”榮譽稱號
2. 2011年獲得山東農業大學“巾幗建功先進個人”榮譽稱號
3. 2009年獲得山東農業大學優秀學士學位論文（指導教師）
4. 2004年獲得山東省優秀博士畢業生花金屬硫蛋白基因
研究專利
1. 棉花Na+/H+反向轉運蛋白基因及其套用，專利號：ZL02151530.1
2. 棉花金屬硫蛋白基因GhMT1序列及其克隆與套用，專利號：ZL200510104301.X
3. 一種棉花促分裂原蛋白激酶基因GhMAPK6及其套用，專利號：ZL201010590833.X
4. 一種可提高轉基因作物細菌抗性的基因GhMKK5及其套用，ZL201010590843.X
發表論文
1. Caili Wang, Shicai Zhang, Shengdong Qi, Chengchao Zheng, Changai Wu* (2015) Delayed germination of Arabidopsis seeds under chilling stress by overexpressing an abiotic stress inducible GhTPS11. GENE, doi: 10.1016/j.gene.2015.08.056.
2. Rui Xu, Yuhan Wang, Hao Zheng, Wei Lu, Changai Wu, Jinguang Huang, Kang Yan, Guodong Yang and Chengchao Zheng. (2015) Salt-induced transcription factor MYB74 is regulated by the RNA-directed DNA methylation pathway in Arabidopsis. Journal of Experimental Botany, 66(19): 5997-6008.
3. Fengjuan Jia, Chunyan Wang, Jinguang Huang, Guodong Yang, Changai Wu and Chengchao Zheng. (2015) SCF E3 ligase PP2-B11 plays a positive role in response to salt stress in Arabidopsis. Journal of Experimental Botany, 66(15): 4683–4697.
4. Changtian Chen, Changai Wu1, Jiaming Miao, Yunxue Lei, Dongxiao Zhao, Dan Sun, Guodong Yang, Jinguang Huang and Chengchao Zheng. (2014) Arabidopsis SAG protein containing the MDN1 domain participates in seed germination and seedling development by negatively regulating ABI3 and ABI5. Journal of Experimental Botany, 65(1): 35-45.
5. Nana Ma, Hailong Feng, Xia Meng, Dong Li, Dongyue Yang, Changai Wu*, Qingwei Meng*. (2014) Overexpression of tomato SlNAC1 transcription factor alters fruit pigmentation and softening. BMC Plant Biology, 14:351.
6. Yunxue Lei, Yaru Fu, Changtian Chen, Jinguang Huang, Changai Wu*. (2014) Overexpression of Arabidopsis wali7 domain-containing protein ASR produces auxin-mediated short-root phenotype. Journal of Plant Growth Regulation, 33:355-363.
7. Yanjie Li, Yi Fang, Yaru Fu, Jinguang Huang, Changai Wu*, Chengchao Zheng*. (2013) NFYA1 is involved in regulation of postgermination growth arrest under salt stress in Arabidopsis. PLoS One, 8(4): e61289.
8. Peng Liu, Kang Yan, YunXue Lei, Rui Xu, YueMin Zhang, Guodong Yang, Jinguang Huang, Changai Wu*, Chengchao Zheng*. (2013) Transcript profiling of microRNAs during the early development of the maize brace root via Solexa sequencing. Genomics, 101: 149-156.
9. Kang Yan, Peng Liu, Chang-Ai Wu, Guo-Dong Yang, Rui Xu, Qian-Huan Guo, Jin-Guang Huang, and Cheng-Chao Zheng. (2012) Stress-Induced Alternative Splicing Provides a Mechanism for the Regulation of MicroRNA Processing in Arabidopsis thaliana. Molecular Cell, 48: 521-531.
10. Wei Lu, Xiaoli Tang, Yanqing Huo, Rui Xu, Shengdong Qi, Jingguang Huang, Chengchao Zheng, Changai Wu*. (2012) Identification and characterization of fructose 1,6-bisphosphate aldolase genes in Arabidopsis reveals a gene family with diverse responses to abiotic stresses. Gene, 503: 65-74.
11. Gang Wang, Qingguo Zhu, Qingwei Meng, Changai Wu*. (2012) Transcript profiling during salt stress of young cotton (Gossypium hirsutum) seedlings via Solexa sequencing. Acta Physiologiae Plantarum, 34:107-115.
12. Yanjie Li, Yaru Fu, Jingguang Huang, Changai Wu*, Chengchao Zheng*. (2011) Transcript profiling during the early development of the maize brace root via Solexa sequencing. The FEBS Journal, 278: 156-166.
13. Ruirui Xu, Shengdong Qi, Longtao Lu, Changtian Chen, Changai Wu*, Chengchao Zheng*. (2011) A DExD/H box RNA helicase is important for potassium deprivation responses and tolerance in Arabidopsis thaliana. The FEBS Journal, 278(13): 2293-2306.
14. Jing Shi, Liang Zhang, Hailong An, Changai Wu*, Xingqing Guo*. (2011) GhMPK16, a novel stress-responsive group D MAPK gene from cotton, is involved in disease resistance and drought sensitivity. BMC Molecular Biology, 12:22.
15. Yanjie Li, Yaru Fu, Lusha Ji, Changai Wu*, Chengchao Zheng*. (2010). Characterization and expression analysis of the Arabidopsis mir169 family. Plant Science, 178: 271-280.
16. Pei Liu, Guodong Yang, Hong Li, Changai Wu*. (2010) Overexpression of NHX1s in transgenic Arabidopsis enhances photoprotection capacity in high salinity and drought conditions. Acta Physiologiae Plantarum, 32: 81-90.
17. Hanhua Liu, Xin Tian, Yanjie Li, Changai Wu*, Chengchao Zheng*. (2008) Microarray-based Analysis of Stress-regulated microRNAs in Arabidopsis thaliana. RNA, 14: 836-843.
18. Changai Wu, Guodong Yang, Qingwei Meng, Chengchao Zheng. (2004) The cotton GhNHX1 gene encoding a novel putative tonoplast Na+/H+ antiporter plays an important role in salt stress. Plant & Cell Physiology, 45: 600-607.
