王賢純[湖南師範大學教授]

王賢純[湖南師範大學教授]
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湖南師範大學生命科學學院二級教授,博、碩士生導師。

基本信息

個人簡介

1959年4月出生,1977年參加工作,1982年獲學士學位,1986年獲碩士學位,1998年獲博士學位。現為湖南師範大學二級教授,博、碩士生導師;湖南省生物化學與分子生物學會理事,中國生物化學與分子生物學會第三屆蛋白質組學專業委員會委員;蛋白質化學與發育生物學教育部重點實驗室副主任、第三屆學術委員會委員;湖南省生物化學與分子生物學重點實驗室、教育部蛋白質化學與發育生物學重點實驗室和省部共建國家重點實驗室培育基地主要學術帶頭人之一;湖南師範大學第六屆“十佳師德標兵”。長期致力於生物化學及相關學科的教學與教研、科學研究、學科建設與人才培養及實驗室建設工作。主講生物化學和蛋白質技術等課程,曾獲生命科學學院教師教學競賽一等獎和湖南師範大學第四屆青年教師課堂教學藝術競賽一等獎。一直擔任生物化學與分子生物學系主任,具體負責該系的本科教學及相關工作,帶領該系進入湖南師範大學“三育人”先進單位行列。主要從事蛋白質化學、蛋白質組學和天然蛋白質多肽毒素等方面的研究。多次應邀在國際國內學術會議上做學術報告。曾先後前往美、英、韓國和越南參加短期學習和學術交流。主持國家863課題和科技部重大基礎研究前期研究專項課題各1項,國家自然科學基金項目課題4項,教育部高等學校博士學科點專項科研基金課題2項,湖南省自然科學基金重點課題和一般課題等省級課題4項。以通訊作者或第一作者在 J Proteome Res, Mol Brain, PloS One, Toxins, Electrophoresis, Int J Biol Macromol,生物化學與生物物理學報和中國生物化學與分子生物學報等國內外重要學術刊物上發表科研和教研論文90餘篇,獲授權國家發明專利2項,受理1項, 通過省級專家鑑定成果1項。指導培養博士、碩士研究生30餘名。參編著作4部。作為主要完成人之一獲2004年和2007年湖南省科技進步一等獎。

代表性論著

1. Xiaozhen Peng, Zhipan Dai, Qian Lei, Long Liang, Shuai Yan and Xianchun Wang. Cytotoxic and apoptotic activities of black widow spiderling extract against HeLa cells. Exp Ther Med. 2017, (In proof)

2. Tinayao Guo, Zhigui Duan, Jia Chen, Chunliang Xie, Ying Wang, Ping Chen, and Xianchun Wang. Pull-down combined with proteomic strategy reveals functional diversity of synaptotagmin I. PeerJ, 2017, 5:e2973.

3. Xia Tang, Chunliang Xie, Ying Wang, Xianchun Wang. Localization of Rab3A-binding site on C2A domain of synaptotagmin I to reveal its regulatory mechanism. Int J Biol Macromol. 2017, 96:736-742.

4. Dehong Xu and Xianchun Wang. Transcriptome Analysis to Understand the Toxicity of Latrodectus tredecimguttatus Eggs. Toxins, 2016, 8(12):378-400.

5. Xia Tang, Jia Chen, Ying Wang, Xianchun Wang. Gene cloning, expression and polyclonal antibody preparation of Rab3A for protein interaction analysis. SpringerPlus, 2016, 5(1):1705-1711.

6. Shuai Yan and Xianchun Wang. Recent Advances in Research on Widow Spider Venoms and Toxins. Toxins, 2015, 7, 5055–5067.

7. Hai Yu, Hui Liu, Yi-Zhong Yan, Zhi-Pan Dai, Shuai Yan, Jiang-Lin Li, Zhi-Gui Duan, Zhen Liu, Xian-Chun Wang. Development and application of wide-range gradient gel electrophoresis to proteome analysis. Anal Methods. 2015, 7:8109-8115.

8. Si-Ling FU, Jiang-Lin LI, Jia CHEN, Qiu-Ting WANG, Jian-Jun LI, Xian-Chun WANG1. Extraction and identification of membrane proteins from black widow spider eggs. Zool Res. 2015,36(4): 248-254.

9. Qian Lei, Hai Yu, Xiaozhen Peng, Shuai Yan, Jirong Wang, Yizhong Yan,

Xianchun Wang. Isolation and Preliminary Characterization of Proteinaceous Toxins with Insecticidal and Antibacterial Activities from Black Widow Spider ( L. tredecimguttatus) Eggs. Toxins, 2015,7:886-899.

10. Chen J, Dai Z, Lei Q, Yan S, Wang Y, Wang X.Effects of huwentoxin-I on catecholamines in cultured PC12 cells. Toxicol Mech Methods. 2015, 25(4):241-8.

11. Yizhong Yan, Jianjun Li, Yiya Zhang, Xiaozhen Peng, Tianyao Guo, Jirong Wang, Weijun Hu, Zhigui Duan and Xianchun Wang. Physiological and biochemical characterization of egg extract of black widow spiders to uncover molecular basis of egg toxicity. Biol Res. 2014, 17-27.

12 . Hai Yu, Hui Liu, Yizhong Yan, Zhigui Duan and Xianchun Wang . Detection and identification of huwentoxin-IV interacting proteins with biotin-avidin chemistry combined with mass spectrometry. J Venom Anim Toxins Incl Trop Dis. 2014, 20:18-26 .

13 . Peng X, Zhang Y, Liu J, Yu H, Chen J, Lei Q, Wang X, Liang S. Physiological and Biochemical Analysis to Reveal the Molecular Basis for Black Widow Spiderling Toxicity. J Biochem Mol Toxicol. 2014,28(5):198-205.

14 . Xie C, Li J, Guo T, Yan Y, Tang C, Wang Y, Chen P, Wang X, Liang S. Rab3A is a new interacting partner of synaptotagmin I and may modulate synaptic membrane fusion through a competitive mechanism.Biochem Biophys Res Commun. 2014,444(4): 491-495.

15. Li J, Yan Y, Yu H, Peng X, Zhang Y, Hu W, Duan Z, Wang X, Liang S. Isolation and identification of a sodium channel-inhibiting protein from eggs of black widow spiders.Int J Biol Macromol. 2014, 65:115-120.

16. Xie C, Su H, Guo T, Yan Y, Peng X, Cao R, Wang Y, Chen P, Wang X, Liang S. Synaptotagmin I delays the fast inactivation of Kv1.4 channel through interaction with its N-terminus.Mol Brain. 2014, 7(1):4-16.

17. Jianjun Li, Hai Yu, Zhigui Duan, Xianchun Wang. A sample preparation method for micro-scale membrane proteome analysis. Sam Prep. 2013, 28-36.

18. Yi-Zhong Yan, Hui Liu, Rong Lv, Sha Huang, Ji-Rong Wang, Tian-Yao Guo, Qiong Zhou and Xian-Chun Wang. Biotin labeling and receptor analysis of huwentoxin-I. Anal Methods, 2013, 5, 3888-3894.

19. Jianjun Li, Yizhong Yan, Jirong Wang, Tianyao Guo, Weijun Hu, Zhigui Duan, Xianchun Wang, and Songping Liang. Purification and Partial Characterization of a Novel Neurotoxic Protein from Eggs of BlackWidow Spiders (Latrodectus tredecimguttatus). J Biochem Mol Toxicol. 2013, 27(7): 337-342.

20. Yong Lin, Linju Huo, Zhonghua Liu, Jianglin Li, Yi Liu, Quanze He, Xianchun Wang, Songping Liang.Sodium Laurate, a Novel Protease- and Mass Spectrometry-Compatible Detergent for MassSpectrometry-Based Membrane Proteomics. PLoS One 2013, 8(3) : e59779.

21. Lin Y, Liu H, Liu Z, Liu Y, He Q, Chen P, Wang X, Liang S. Development and evaluation of an entirely solution-based combinative sample preparation method for membrane proteomics.Anal Biochem. 2013, 432 (1): 41-48.

22. Li J, Liu H, Duan Z, Cao R, Wang X, Liang S. Protein Compositional Analysis of the Eggs of Black Widow Spider (L. tredecimguttatus): Implications for the Understanding of Egg Toxicity. J Biochem Mol Toxicol. 2012, 26(12):510-515.

23. Xianchun Wang, Songping Liang. Sample preparation for the analysis of membrane proteomes by mass spectrometry. Protein & Cell. 2012, 3(9):661-668.

24. Yong Lin, Hui Liu, Zhonghua Liu,Xianchun Wang, Songping Liang. Shotgun analysis of membrane proteomes using a novel combinative strategy of solution-based sample preparation coupled with liquid chromatography-tandem mass spectrometry. J Chromatogr B, 2012, 901:18-24.

25. Xianchun Wang and Songping Liang. Gel-absorption-based sample preparation method for shotgun analysis of membrane proteome. Methods Mol Biol. 2012, 869:385-392.

26. Jian Zhou, Deng Bi, Yong Lin, Ping Chen, Xianchun Wang, Songping Liang. Shotgun proteomics and network analysis of ubiquitin-related proteins from human breast carcinoma epithelial cells. Mol Cell Biochem. 2012, 359: 375-384.

27. Yi Liu, Yong Lin, Yizhong Yan, Jianjun Li, Quanze He, Ping Chen, Xianchun Wang, Songping Liang. Electrophoretically-driven SDS removal and protein fractionation in the shotgun analysis of membrane proteomes. Electrophoresis, 2012, 33: 316-324.

28. Jian Zhou, Jianlin Li, jianjun Li, Ping Chen, Xianchun Wang X, Songping Liang S. Dried polyacrylamide gel absorption: A method for efficient elimination of the interferences from SDS-solubilized protein samples in mass spectrometry-based proteome analysis. Electrophoresis, 2010 , 31 : 3816-3822.

29. Yong Lin, Yi Liu, Jianjun Li, Yan Zhao, Quanze He, Wenjun Han, Ping Chen, Xianchun Wang , Songping Liang. Evaluation and optimization of removal of an acid-insoluble surfactant for shotgun analysis of membrane proteome.Electrophoresis, 2010 , 31 : 2705-2713.

30. Zhou J, Xiong J, Li J, Huang S, Zhang H, He Q, Lin Y, Chen P, Wang X, Liang S. Gel absorption-based sample preparation for the analysis of membrane proteome by mass spectrometry.Anal Biochem. 2010 , 404(2):204-10.

31. Jian Zhou, Sha Huang, Deng Bi, Hai Zhang, Jianglin Li, Yong Lin, Ping Chen, Xianchun Wang, Songping Liang.Analysis of integral membrane proteins by heat gel-embedment combined with improved in-gel digestions. Electrophoresis, 2009,30 (23):4109-4117.

32. Xiong Xia, Shen Jian-Ying, Li Jian-Jun, Zhang Hai, Wang Xian-Chun, Liang Song-Ping. Aqueous polymer two-phase partition for the proteomic analysis of plasma membrane from rat dorsal root ganglion neurons. Prog Biochem Biophys, 2009, 36(11):1458-1468.

33. Xia Xiong, Sha Huang, Hai Zhang, Jian-jun Li, Jian-Ying Shen, Jixian Xiong, Yong Lin, Liping Jiang, Xian-Chun Wang, Song-Ping Liang. Enrichment and proteomic analysis of plasma membrane from rat dorsal root ganglion neurons. Proteome Sci. 2009,7:41-50.

34. Yong Lin, Yan Li, Yi Liu, Wenjun Han, Quanze He, Jianglin Li, Ping Chen, Xianchun Wang , Songping Liang . Improvement of gel-separated protein identification by DMF-assisted digestion and peptide recovery after electroblotting. Electrophoresis, 2009,30(20 ): 3626-3635.

35. Zhigui Duan, Xiaojun Yan, Rui Cao, Zhen Liu, Xianchun Wang and Songping Liang.Proteomic analysis of Latrodectus tredecimguttatus venom for uncovering potential latrodectism-related proteins. J Biochem Mol Toxicol. 2008,22(5):328-336.

36. Yong Lin, Jian Zhou, Deng Bi, Ping Chen, Xianchun Wang, Songping Liang . Sodium deoxycholate- assisted tryptic digestion and identification of proteolytically resistant proteins. Anal Biochem. 2008, 377(2): 259 - 266.

37. Jian Zhou, Yong Lin, Xingcan Deng, Jianying Shen, Quanyuan He, Ping Chen,Xianchun Wang, Songping Liang. Development and application of a two-phase, on-membrane digestion method in the analysis of membrane proteome. J Proteome Res. 2008, 7 : 1778 - 1783.

38. X.C. Wang, Z.G Duan, J.Yang, X.J.Yan, H. Zhou, X.Z.He, and S.P.Liang. Physiological and biochemical analysis of L. tredecimguttatus venom collected by electrical stimulation. J Physiol Biochem. 2007, 63(3):221-230 。

39. Jian Zhou,Tieyang Zhou, Rui Cao, Zhen Liu, Jianying Shen, Ping Chen, Xianchun Wang, and Songping Liang.Evaluation of the application of sodium deoxycolate to proteomic analysis of rat hippocaampal plasma membrane. J Proteome Res.2006, 5, 2547-2553。

40. Duan ZG,Yan XJ,He XZ,Zhou H,Chen P,Cao R,Xiong JX,Hu WJ,Wang XC,Liang SP.Extraction and protein component analysis of venom from the dissected venom glands of Latrodectus tredecimguttatus. Comp Biochem Physiol. Part B. 2006,145 ( 3-4 ): 350-357 。

41. Wang lin, Zhou Tieyang, Tang Xing, Wang Xianchun,Liang Songping.Purification and characterization of a novelα-amylase inhibitor from wild Amaranth (Amaranthus paniculatus) weeds. Chin J of Biochem Mol Biol, 2004,20(4):434-439 。

42. WANG Xian-Chun, CHEN Ping, LIANG Song-Ping. Liquid chromatography-tandem mass spectrometry analysis of a synthetic nonapeptide and its main by-products. Chin J Biochem Mol Biol, 2003 , 19 ( 5 ): 576-581.

43. 王賢純,梁宋平。間斑寇蛛毒素的蛋白質化學與蛋白質組學研究新進展。生物工程學報, 2014,30(7):1036-1043。

44. 王賢純 陳 平王迎 王美遲 曾雄智 梁宋平。生物化學雙語教學的課堂實踐與探索。教學研究。2013,36(5):57-60。

45. 王賢純,陳 平,王 迎,王美遲,曾雄智,段志貴,劉 珍,梁宋平。生物化學雙語教材建設的探討。高師理科學刊。2012,32(1):101-103。

46. 段志貴,楊靜,晏曉軍,曾雄智,王賢純,梁宋平。間斑寇蛛粗毒液的生理生化分析及兩種采毒方法的比較。動物學研究,2009,30(4):381-388。

47. 林勇,周健,畢登,陳平,王賢純,梁宋平。脫氧膽酸鈉(SDC)促進的蛋白質的酶解與鑑定。中國科技論文線上精品論文。2009,2(7):669-673。

48. 王賢純, 陳平, 王迎,曾雄智, 王美遲,梁宋平。關於進一步推進高校本科雙語教學的幾點思考。高等教育研究學報,2008,31(2):36-37。

49. 王賢純, 陳平, 曾雄智, 王美遲。 高校雙語教學的實施策略。北京大學學報(哲學社會科學版),2007,S2:68-69。

50. 楊靜,段志貴,周紅,晏曉軍,何小珍,王賢純,梁宋平。間斑寇蛛粗毒的生物學特性。動物學報 ,2007,53(4):682-688。

51. 段志貴,晏曉軍,鄧星燦,王賢純。間斑寇蛛幼蛛過冬及其早期飼養管理。生態學雜誌,2007,26(3):363-367 。

52 .晏曉軍,段志貴,周遠峰,王賢純,梁宋平。間斑寇蛛(L tredecimguttatus)粗毒抗血清製備及其活性分析。蛛形學報,2006,22(2)80-84。

53. 周鐵楊,周健,曹銳,沈建英, 陳平, 王賢純。大鼠海馬細胞質膜的分離及其蛋白質組學分析。中國生物化學與分子生物學報,2006,22(9):733-743。

54. 周鐵楊,周健,雄繼先,王賢純。基於等電聚焦-反相HPLC的虎紋捕鳥蛛毒素組學的初步研究。中國生物化學與分子生物學報,2006,22(10):823-831。

55. 范春明,王賢純,梁宋平。兩種蜘蛛多肽毒素的質譜法序列分析。質譜學報。2005,26(2):65-70。

56. 徐峽,熊霞,李冬玲,肖玉成,王賢純,梁宋平。海南捕鳥蛛毒素-IV突變體的固相合成和生理活性分析。生物工程學報,2005,21(1):92-96。

57. 曹銳,張麗軍,聶松,王賢純,梁宋平。多維色譜-串聯質譜聯用技術分離和鑑定小鼠肝臟質膜蛋白質。中國生物化學與分子生物學報。2005,21(1):134-142。

58. 周紅,何小珍,段志貴,晏曉軍,胡紹海,王賢純,梁宋平。間斑寇蛛(L tredecimguttatus) 室內飼養及活體采毒方法的初步探討。蛛形學報,2005,14(1):58-63。

59. 王賢純,梁宋平。虎紋捕鳥蛛毒素-I Lys13突變對生物學活性的影響。自然科學進展。2004,14(2):227-231 。

60. 王賢純,梁宋平。反相高效液相色譜/電噴霧質譜法分析化學合成七肽粗產物。分析化學。2004,32(9):1219-1222。

61. 王賢純,范春明,唐新科,陳平,梁宋平。牛血清白蛋白胰蛋白酶酶解產物的色譜-質譜聯用分析及其三種資料庫搜尋鑑定方法的比較。中國生物化學與分子生物學報。2004,20(3):393-398。

62. 王賢純,梁宋平。化學合成ω-芋螺毒素MVIIA的復性與質譜分析。動物學研究。2003,24(2):121-126。

63 .王賢純。蜈蚣的藥用研究進展。動物學雜誌,2002,37(3):88-91。

64. 王賢純,梁宋平。電噴霧串聯質譜圖的疊合與多肽序列分析。生物化學與生物物理學報。2001,33(6):665-670。

65. 王賢純,梁宋平。化學合成虎紋捕鳥蛛毒素-I的色譜行為及分離純化。色譜。2001,19(2):101-104。

66. 王賢純,梁宋平,羅澤民。虎紋捕鳥蛛毒素-I單殘基突變體R20A-HWTX-I的化學合成與性質分析。生物工程學報。2000,16(4):490-494。

67. 王賢純,梁宋平,羅澤民。構建同時攜帶HWTX-I和AAI活性位點的多肽嵌合體。生物化學與生物物理學報,2000,32(3):274-280。

68. 王賢純,梁宋平,羅澤民。虎紋捕鳥蛛毒素-I突變體A1Y-HWTX-I的固相合成和生物學活性測定。中國生物化學與分子生物學報,2000,16(3):357-362。

69. 王賢純,梁宋平。不同溶劑對合成多肽的洗脫效果比較。化學通報(網路版)。1999,c00083。

70. 王賢純,梁宋平。豆科植物籽實的昆蟲消化道α-澱粉酶抑制劑的研究(II)。生物技術。1995,5(2):29-31。

71. 王賢純,梁宋平。豆科植物籽實的昆蟲消化道α-澱粉酶抑制劑的研究(I)。生物技術。1994,4(5):18-21。

72. 王賢純,謝錦雲,單茶秀,戚志仁。薑黃的綜合利用—水蒸汽蒸餾法提取薑黃油。食品科學。1993,8:49-51。

73. 王賢純,謝錦雲,單茶秀,戚志仁。薑黃的綜合利用—乙醇循環法提取薑黃素。食品科學。1993,9:45-47。

74. 王賢純。薑黃色素的穩定性。食品與發酵工業。1994,1:63-66。

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