![周景文[江南大學教授]](/img/2/bc8/nBnauM3XxgDM3UzMyATN1kTO0UTMyITNykTO0EDMwAjMwUzLwUzL3czLt92YucmbvRWdo5Cd0FmLwE2LvoDc0RHa.jpg "周景文[江南大學教授]")
個人簡介
，男，1982.4.29出生，職 稱 教授。
學術簡介
以維生素C和苯丙素類化合物為目標產物，闡明微生物高效異源合成植物天然產物的生理機制，並通過計算機輔助的代謝途徑設計、代謝途徑單元模組化調控、輔因子平衡與再生調控、反義RNA工程等策略，在微生物中實現來源於植物的高附加值天然產物的高效異源合成。
主要論著
前五年在Biotechnology Advances、Metabolic Engineering、Eukaryotic Cell和Applied and Environmental Microbiology等生物工程和微生物生理學權威學術期刊上發表與本項目相關的研究論文和綜述45篇，授權發明專利14項，參編髮酵工程專業著作4本。
代表性著作和論文如下:
[1] Saijie Zhu, Junjun Wu, Guocheng Du, Jingwen Zhou*, Jian Chen. Efficient synthesis of eriodictyol from L-tyrosine in Escherichia coli. Applied and Environmental Microbiology. 2014, 10(80): 3072-3080. (2012 IF= 3.678)
[2] Lili Gao, Yudong Hu, Jie Liu, Guocheng Du, Jingwen Zhou*, Jian Chen. Stepwise metabolic engineering of Gluconobacter oxydans WSH-003 for the direct production of 2-keto-L-gulonic acid from D-sorbitol. Metabolic Engineering. 2014, 24: 30–37. (2012 IF= 6.859)
[3] Shaohui Zhao, Xinrui Zhao, Huijun Zou, Jianwei Fu, Jian Chen, Jingwen Zhou, Guocheng Du. Comparative proteome analysis of Saccharomyces cerevisiae grown under different nitrogen source conditions. Journal of Proteomics. 2014, 101: 102-112 (2012 IF=4.088)
[4] Jingwen Zhou, Guocheng Du, Jian Chen. Novel fermentation processes for manufacturing plant natural products. Current Opinion in Biotechnology. 2014. 25: 17-23. (2012 IF= 7.86)
[5] Xinrui Zhao, Guocheng Du, Huijun Zou, Jianwei Fu, Jingwen Zhou, Jian Chen. Metabolic engineering of the regulators in nitrogen catabolite repression to reduce the production of ethyl carbamate in a model rice wine system. Applied and Environmental Microbiology. 2014, 80(1): 392-398. (2012 IF= 3.678)
[6] Lili Gao, Jie Liu, Guocheng Du, Jingwen Zhou, Jian Chen. Characterization of a group of pyrroloquinoline quinone-dependent dehydrogenases that are involved in the conversion of L-sorbose to 2-Keto-L-gulonic acid in Ketogulonicigenium vulgare WSH-001. Biotechnology Progress. 2013. 29(6):1398-1404 . (2012 IF= 1.853)
[7] Jidong Liu, Weiping Zhang, Guocheng Du, Jian Chen, Jingwen Zhou. Overproduction of geraniol by enhanced precursor supply in Saccharomyces cerevisiae. Journal of Biotechnology. 2013, 168(4): 446-451. (2012 IF= 3.183)
[8] Xinrui Zhao, Huijun Zou, Jianwei Fu, Jingwen Zhou, Jian Chen, Guocheng Du. Nitrogen regulation involved in the accumulation of urea in Saccharomyces cerevisiae used in rice wine production. Yeast. 2013. 30(11): 437-447. (2012 IF= 1.955)
[9] Junjun Wu, Peiran Liu, Yongming Fan, Han Bao, Guocheng Du, Jingwen Zhou, Jian Chen. Multivariate modular metabolic engineering of Escherichia coli to produce resveratrol from L-tyrosine. Journal of Biotechnology. 2013, 167: 404-411. (2012 IF= 3.183)
[10] Jingwen Zhou, Qiaoshuang Zheng, Jie Liu, Jianmin Huang, Guocheng Du, Jian Chen. Indigenous plasmids of Bacillus megaterium WSH-002 involved in mutualism with Ketogulonicigenium vulgare WSH-001. Plasmid . 2013, 70(2):240-246. (2012 IF=1.276)
[11] Xinrui Zhao, Guocheng Du, Huijun Zou, Jianwei Fu, Jingwen Zhou*, Jian Chen. Progress in preventing the accumulation of ethyl carbamate in alcoholic beverages. Trends in Food Science and Technology. 2013, 32: 97-107. (2012 IF=4.135)
[12] Xiaobei Wang, Jie Liu, Guocheng Du, Jingwen Zhou, Jian Chen. Efficient production of L-sorbose from D-sorbitol by whole cell immobilization of Gluconobacter oxydans WSH-003. Biochemical Engineering Journal. 2013, 77: 171–176. (2012 IF= 2.579)
[13] Jidong Liu, Yibo Zhu, Guocheng Du, Jingwen Zhou, Jian Chen. Response of Saccharomyces cerevisiae to D-limonene-induced oxidative stress. Applied Microbiology and Biotechnology. 2013, 97(14): 6467-6475. (2012 IF=3.689)
[14] Yongkun Lv, Xinrui Zhao, Long Liu, Guocheng Du, Jingwen Zhou*, Jian Chen. A simple procedure for protein ubiquitination detection in Saccharomyces cerevisiae: Gap1p as an example. Journal of Microbiological Methods. 2013, 94: 25-29 . (2012 IF=2.161)
[15] Jingwen Zhou, Zhenghong Xu, Shouwen Chen. Simulation and prediction of the thuringiensin abiotic degradation processes in aqueous solution by a radius basis function neural network model. Chemosphpere. 2013. 91(4): 442-447. (2012 IF= 3.137)
[16] Shimon Bershtein, Wanmeng Mu, Adrian Serohijos, Jingwen Zhou, Eugene I. Shakhnovich. Protein quality control shapes the fitness effects of mutations in E. coli. Molecular Cell. 2013. 49(1): 133-144. (2012 IF= 15.28)
[17] Jidong Liu, Yibo Zhu, Guocheng Du, Jingwen Zhou, Jian Chen. Exogenous ergosterol protects Saccharomyces cerevisiae from D-Limonene stress. Journal of Applied Microbiology. 2013. 114(2):482-491. (2012 IF=2.196)
[18] Junjun Wu, Guocheng Du, Jingwen Zhou, Jian Chen. Metabolic engineering of Escherichia coli for (2S)-pinocembrin production from glucose by a modular metabolic strategy. Metabolic Engineering. 2013. 16:48-55. (2012 IF= 6.859)
[19] Hua Liu, Guocheng Du, Jianghua Li, Jingwen Zhou, Jian Chen. Enhanced production of a-cyclodextrin glycosyltransferase in Escherichia coli by codon usage optimization. Journal of Industrial Microbiology & Biotechnology . 2012. 39(12): 1841-1849. (2012 IF=2.321)
[20] Xiaoxia Yin, Catherine Madzak, Guocheng Du, Jingwen Zhou, Jian Chen. Enhanced a-ketoglutarate production in Yarrowia lipolytica WSH-Z06 by regulation of the pyruvate carboxylation pathway. Applied Microbiology and Biotechnology. 2012. 96(6): 1527-1537. (2012 IF=3.689)
[21] Jingwen Zhou, Hua Liu, Jianghua Li, Guocheng Du, Jian Chen. Production of a-cyclodextrin glycosyltransferase in Bacillus megaterium MS941 by systematic codon usage optimization. Journal of Agricultural and Food Chemistry . 2012, 60(41), 10285–10292. (2012 IF=2.906)
[22] Jingwen Zhou, Xiaoxia Yin, Catherine Madzak, Guocheng Du, Jian Chen. Enhanced a-ketoglutarate production in Yarrowia lipolytica WSH-Z06 by alteration of the acetyl-CoA metabolism. Journal of Biotechnology. 2012. 161: 257-264. (2012 IF= 3.183)
[23] Feifei Hu, Jidong Liu, Guocheng Du, Zhaozhe Hua, Jingwen Zhou, Jian Chen. The key cytomembrane ABC transporters of Saccharomyces cerevisiae failed to improve the tolerance to D-limonene. Biotechnology Letters. 2012. 34(8): 1505-1509. (2012 IF= 1.853)
[24] Lili Gao, Jingwen Zhou, Jie Liu, Guocheng Du, Jian Chen. Draft Genome Sequence of Gluconobacter oxydans WSH-003, an Extremely Saccharide and Alditol-Tolerant Strain. Journal of Bacteriology. 2012. 194(16) 4455-4456 . ( 2012 IF=3.194 )
[25] Yibo Zhu, Jie Liu, Guocheng Du, Jingwen Zhou, Jian Chen. A high throughput method to screen companion bacterium for 2-keto-L-gulonic acid biosynthesis by co-culture Ketogulonigenium vulgare. Process Biochemistry. 2012. 47: 1428-1432 . (2012 IF=2.414).
[26] Zongzhong Yu, Guocheng Du, Jingwen Zhou, Jian Chen. Enhanced a-ketoglutaric acid production in Yarrowia lipolytica WSH-Z06by an improved integrated fed-batch strategy. Bioresource Technology. 2012. 114: 597-602. ( 2012 IF=4.75 )
[27] Yibo Zhu, Jie Liu, Guocheng Du, Jingwen Zhou, Jian Chen. Sporulation and stable spore of Bacillus megateriumenhance Ketogulonigenium vulgarepropagation and 2-keto-L-gulonic acidbiosynthesis. Bioresource Technology. 2012. 107: 399-404. ( 2012 IF=4.75 )
[28] Zhengxiong Zhou, Guocheng Du, Zhaozhe Hua, Jingwen Zhou, Jian Chen. Optimization of fumaric acid production by Rhizopus delemar based on the morphology formation. Bioresource Technology. 2011, 102: 9345-9349. (2012 IF=4.75)
[29] Jingwen Zhou, Liming Liu, Jian Chen. Improved ATP supply enhances acid tolerance of Candida glabrata during pyruvic acid production. Journal of Applied Microbiology. 2011, 110(1):44-53. (2012 IF=2.196)
[30] Jingwen Zhou, Liming Liu, Jian Chen. Method to purify mitochondrial DNA directly from yeast total DNA. Plasmid. 2010, 64(3):196-199. (2012 IF=1.276)
[31] Jingwen Zhou, Haiyan Zhou, Guocheng Du, Liming Liu, Jian Chen. Screening of a thiamine auxotrophic yeast for α-ketoglutaric acid overproduction. Letters in Applied Microbiology. 2010, 51(3):264-271. (2012 IF=1.629)
[32] Jingwen Zhou, Liming Liu, Jian Chen. Mitochondrial DNA heteroplasmy in Candida glabrata after mitochondrial transformation. Eukaryotic Cell. 2010, 9(5): 806-814. (2012 IF=3.586)
[33] Jingwen Zhou, Liming Liu, Zhongping Shi, Guocheng Du, Jian Chen. ATP in Current Biotechnology: Regulation, Application and Perspectives. Biotechnology Advances. 2009, 27(1): 94-101. (2012 IF= 9.599)
[34] Jingwen Zhou, Luxi Huang, Liming Liu Jian Chen. Enhancement of pyruvate productivity by inducible expression of a FF-ATPase inhibitor INH1 in Torulopsis glabrata CCTCC M202019. Journal of Biotechnology. 2009, 144(2): 120-126. (2012 IF= 3.183)
[35] Jingwen Zhou, Zhiyao Dong, Liming Liu, Guocheng Du, Jian Chen. A reusable method for construction of non-marker large fragment deletion yeast auxotroph strains: a practice in Torulopsis glabrata. Journal of Microbiological Methods. 2009, 76(1): 70–74. (2012 IF=2.161)
[36] Jingwen Zhou, Yafei Chang, Zhenghong Xu, Ziniu Yu, Shouwen Chen. Production of thuringiensin by fed-batch culture of Bacillus thuringiensis subsp. darmstadiensis 032 with an improved pH-control glucose feeding strategy, Process Biochemistry. 2007, 42(1): 52-56. (2012 IF=2.414)
[37] 郭洪偉, 堵國成, 陳堅, 周景文*. 利用微生物發酵生產a-酮戊二酸進展. 生物工程學報. 2013, 29(2): 141−152.
[38] 孫明雪, 劉繼棟, 堵國成, 周景文*, 陳堅. 調控釀酒酵母類異戊二烯合成途徑強化芳樟醇合成. 生物工程學報. 2013, 29(6): 751−759.
[39] 殷曉霞, 堵國成, 陳堅, 周景文*. 強化乙醯輔酶A供給對解脂亞洛酵母產a-酮戊二酸的影響. 工業微生物. 2013, 43(2): 45-50.
[40] 余宗鍾, 堵國成, 陳堅, 周景文*. 基於細胞代謝調控的a-酮戊二酸發酵過程放大. 工業微生物. 2013, 43(3): 23-28.
[41] 周景文, 董志姚, 劉立明, 堵國成, 陳堅. 葡萄糖酸鈉對光滑球擬酵母CCTCC M202019能量代謝和丙酮酸積累的影響. 華中農業大學學報 ( 自然科學版 ), 2010, 29(4): 10-15.
[42] 周景文, 徐建, 陳守文, 喻子牛. 基於徑向基神經網路和遺傳算法的聚-γ-谷氨酸發酵培養基最佳化. 食品科學. 2006, 27(10): 270-274.
[43] 周景文，堵國成，陳堅 *. 發酵食品有害氨(胺)類代謝物：形成機制和消除策略. 中國食品學報. 2011, 9: 8-25.
[44] 朱益波, 周景文*，陳堅. 巨大芽孢桿菌與普通生酮基古龍酸菌共培養過程中相互作用分子機制研究進展. 微生物學報. 2012, 52(8):940-947.
[45] 張偉平, 趙鑫銳, 堵國成, 鄒慧君, 傅建偉, 周景文*, 陳堅. 釀酒酵母氮代謝物阻遏效應及其對發酵食品安全的影響. 套用與環境生物技術學報. 2012, 18(05): 862-872.
*: 通訊作者。
科研項目
前五年主持或完成國家自然科學基金、江蘇省自然科學基金和國家/部級重點實驗室開放課題及企業橫向委託項目8項。其中代表性的科研項目如下:
[1] 解脂亞洛酵母酮酸轉運蛋白的生理功能與調控機制. 國家自然科學基金面上項目(21276109). 2013年1月1日-2016年12月1日. 項目主持人；
[2] 微生物高效積累黃酮類化合物的關鍵共性問題研究 . 江南大學自主科研計畫-重點項目(JUSRP51307A), 2013年1月-2015年12月. 項目主持人；
[3] 維生素 C 合成過程吡咯喹啉醌代謝關鍵基因及其調控機制. 教育部新世紀優秀人才支持計畫(NCET-12-0876), 2012年1月-2015年12月. 項目主持人；
[4] 維生素 C 從頭合成關鍵模組功能解析與調控，高等學校全國優秀博士學位論文作者專項資金(2011046)，2012年1月-2016年12月. 項目主持人；
[5] 生化網路重構與維生素生產新菌種 . 863項目(2012AA022103). 2012 年 11 月 1 日 -2015 年 12 月 31 日；
[6] 食品安全全程控制的食品製造過程設計與構建 . 國家重點基礎研究發展計畫(973計畫，2012CB720806). 2012 年 1 月 -2016 年 8 月. 子課題負責人；
[7] 代謝工程改造釀酒酵母合成 D- 檸檬烯的關鍵問題研究. 國家自然科學基金青年科學基金項目 (31000807). 2011年1月1日-2013年12月1日. 項目主持人；
[8] 代謝工程改造釀酒酵母合成 S- 芳樟醇及其發酵過程最佳化. 江蘇省基礎研究計畫(BK201020722). 2010年 7月1日-2013年7月1日. 項目主持人。
榮譽與獎勵
[1] 2013年12月，新型有機酸的發酵法製備技術，陳堅、 周景文、劉龍、李江華、劉立明，2013年中國輕工業聯合會技術發明獎一等獎；
[2] 2012年8月，發酵法生產α-酮戊二酸，堵國成、 周景文、李江華、陳堅、宗偉剛、胡志傑、王立軍，2012年無錫市科技進步獎二等獎；
[3] 2011年11月，光滑球擬酵母中ATP的生理功能與作用機制， 周景文，全國百篇優秀博士學位論文(證書編號：2011046)；
[4] 2011年10月，《發酵過程最佳化原理與技術》，陳堅、堵國成、劉立明、 周景文、劉龍等，中國石油和化學工業聯合會，科技進步獎一等獎(證書編號：2011JBR0516-1-6)；
[5] 2011年2月，工業微生物生理特性與代謝功能研究，陳堅、堵國成、劉立明、吳敬、 周景文、張娟、劉龍、張東旭，江蘇省科技技術獎一等獎(證書編號：2010-1-3-R-5)；
[6] 2010年10月，光滑球擬酵母中ATP的生理功能與作用機制， 周景文，江蘇省2010年度優秀博士論文(證書編號：CX10B064)。
