Storage Method
1. Store in a cool, well - ventilated kept away from the fire, the heat - retaining container sealing should be kept away from the fire, the heat - retaining container sealing should be .
. Store away from oxidizers Store away from oxidizers .
adopt explosion - proof lighting and ventilation facility adopt explosion - proof lighting and ventilation facility . easy to generate sparkle shall be prohibited to use machinery and tools shall be prepared in storage area easy to generate sparkle shall be prohibited to use machinery and tools shall be prepared in storage area .
emergency treatment facilities and suitable housing materials. The use of 25kg net in Cardboard Drum, cardboard boxes or paper bags lined with double polyethylene bag packaging shading, enclosed in a dry place at room temperature under control just save shading, enclosed in a dry place at room temperature under control just save .
2 2.
Synthesis Method
Hydrochloric Acid Esterification Method
1, 2 - chloroethylamine hydrochloride 1, 2 - chloroethylamine hydrochloride . in a 250 - mL flask was added 100 g of concentrated hydrochloric acid, with stirring, slowly add the ethanolamine 61g, warmed to 145 - 150. degree. C., by hydrogen chloride gas escapes to a small amount of HCl; and then distillation, vacuum distillation at 150.d egree. C. lower edges 15h of the reaction, anhydrous to distill; Finally, the reaction liquid was cooled to 70 DEG C, adding 50 g of anhydrous ethanol, cooling, crystallizing, separating, washing with anhydrous ethanol (), and vacuum drying 106 g, yield 91. 3%. 2.Preparation of taurine
66. 2gNa2SO3 were added to a 500 - ml flask, add 250 mL of water and dissolved at 50 for starting the dropwise addition of the above product was dissolved in 15 mL of aqueous solution of 58 g, 6h are completed; and then warmed to 65.d egree. C. reflects 3h, warmed to 90 C. for 4 h and finally refluxed for 1h, the taurine content is reduced and the yield of 94% electrodialysis method for removal of inorganic salts, and then concentrated and crystallized to obtain the taurine 57. 2g, the extraction yield is 91. 5%, the purity is 99. 6%, total yield 83.6%. 2
Sulfuric Acid Esterification Method
1, 2 - aminoethyl hydrogen sulfate ester synthesis 1, 2 - aminoethyl hydrogen sulfate ester synthesis . in a 500 - mL flask was added ethanolamine (36 g, 0.6 mol) and toluene (100 mL), cooled in a water bath and was added dropwise, with stirring 98% sulphuric acid (61 g, 0. 626mol), about 50 min after dropwise addition of cetyl triethyl ammonium chloride (1.4 g, 0. 0039mol), a solvent with carbon tetrachloride (CTC), heated to reflux for 1.5 -2 hours, isolating the theoretical amount of water (about 11 mL), cooling, filtering, washing, drying,2 - aminoethyldisulfate 83g (melting point: 273 - 279 C.) yield by 1%. 2. Synthesis of taurine
in a 500 - mL flask was added sodium sulfite (75 g, 0. 60mlo) and 250 mL of water, under a blanket of nitrogen gas slowly and uniformly added over a hydrogen sulfate ester (28 g, 0.2 mol), After the completion of the reflux is continued for a further 10 - 12h generate taurine; wherein water was distilled off under reduced pressure, 100 mL of concentrated sulfuric acid was added and stirred for about 1 h, the product is completely dissolved; filtering the inorganic salt crystals,20 mL of concentrated hydrochloric acid and twice with filtrate was vacuum - concentrated to half volume is added to a mixture of 95% ethanol 50Ml, i.e. a portion of the crystallization filtrate to freezing in a refrigerator for 1 - 2hr, filtering to obtain a crude product with concentrated hydrochloric acid and recrystallized once to obtain the product (21 g, melting point 298 - 301, and the yield of 85% of the total yield of 83%
.
Thionyl Chloride Method
at 60.d egree. C. with 2 - aminoethanol hydrochloride SOCL2 is calculated according to the formula by reaction of 2 - chloro - 2 - aminoethanol hydrochloride with a yield of 99% as with 350mL37% of hydrochloric acid and dissolved in 800 mL of toluene in the reaction of ethanolamine 244g, 110. degree. C. with azeotropic removal of water, cooled to 50 DEG C, adding 3. 2gNH4Cl catalyst, followed by the dropwise addition of 14mLSOCl2, 2 - aminoethanol hydrochloride
then 53. 7g80% of the product solution was added dropwise to 180 ml, the concentration of 21 4% of sodium sulfite solution and 80 C for 4 h.,The polysiloxanes containing 39 g of an aqueous solution of taurine, electrodialysis desalination, and cooled to 3 c to crystallize for purification to obtain the product with a purity of 95%, 95% yield 
Bio - Extraction
bovine bile hydrolysis or squid, octopus and fish shellfish and mammals flesh and viscera extracted, concentrated, or may be obtained by extraction from cow - bezoar in chemical synthesis or by bovine bile hydrolysis or squid, octopus and fish shellfish and mammals flesh and viscera extracted, concentrated, or may be obtained by extraction from cow - bezoar in chemical synthesis or by . 2 2.
Drug Analysis
Neutralization Titration
Scope of application: The method for the determination of taurine in taurine content Scope of application: The method for the determination of taurine in taurine content . which is applicable to BPCs taurine which is applicable to BPCs taurine .
method principle: the substance dissolved in water, and titrated with sodium hydroxide solution to adjust the pH to 7. 0, and then added to a pre - adjusted to pH 9. 0 of the formaldehyde solution and then titrated with sodium hydroxide until the solution pH value to 9.0, for a duration of 30 minutes, the formaldehyde is added to the solution after the sodium hydroxide solution consumed for titration of the amount was calculated on the basis of the content of taurine reagents: 1), formaldehyde, sodium hydroxide volumetric solution (0.1mol / L) 3, phenolphthalein indicator 4, the reference - phthalate) and instruments:Sample Preparation: 1. The sodium hydroxide volumetric solution (0.1mol / L) solution is prepared by a proper amount of sodium hydroxide, water and shake to dissolve the mixture into a saturated solution, after cooling, polyethylene plastic bottle and allowed to stand for days, clarification and reserved
take a clear and saturated solution of sodium hydroxide in 5 mL, plus new boiling water to make 1000 mL, and mix take a clear and saturated solution of sodium hydroxide in 5 mL, plus new boiling water to make 1000 mL, and mix . calibration: dried at 105.d egree. C. to constant weight of Potassium Acid Phthalate (KAP) about a reference 0. 6g, accurately weighed, in 50 ml freshly boiled water, shaking, so as to dissolve, adding 2 drops of phenolphthalein indicator solution, this droplet, at the end,in such a way that the phthalate is completely dissolved, the solution is titrated to a pink
per 1 mL of sodium hydroxide volumetric solution (0.1mol / L) equivalent to 20 mg of potassium hydrogen phthalate according to this liquid consumed and the amount of potassium hydrogen phthalate, and calculates the concentration of the according to this liquid consumed and the amount of potassium hydrogen phthalate, and calculates the concentration of the .
Storage: Placed in a polyethylene plastic bottle, seal save; plug in the hole 2, the respective insertion holes within the glass tube 1, tube 1 with a soda lime tube, the tube 1 for sucking out liquid using this Storage: Placed in a polyethylene plastic bottle, seal save; plug in the hole 2, the respective insertion holes within the glass tube 1, tube 1 with a soda lime tube, the tube 1 for sucking out liquid using this .
Phenolphthalein Indicator
1 g of phenolphthalein is taken, added with 100 mL of ethanol to dissolve the 1 g of phenolphthalein is taken, added with 100 mL of ethanol to dissolve the . operation includes the steps of: from about 0. 2 g, accurately weighed, dissolved in 50 mL water, was added neutral formaldehyde solution (formaldehyde solution, 5 drops of phenolphthalein indicator was added dropwise, with 0. 1mol / L sodium hydroxide solution to adjust until the solution appears pink) 5 ml of distilled water, neutralize with sodium hydroxide volumetric solution (0.1mol / L)
1 mL of sodium hydroxide volumetric solution (0.1mol / L) is equivalent to 12 mg of C2H7NO3S Note: "Precisely weigh"refers to that the weighted weight shall be as precise as one one - thousandth of the weighed weight Note: "Precisely weigh"refers to that the weighted weight shall be as precise as one one - thousandth of the weighed weight .
"precise measuring" refers to the accuracy of the measured volume shall meet the national standard for the volume of the pipet accuracy requirements "precise measuring" refers to the accuracy of the measured volume shall meet the national standard for the volume of the pipet accuracy requirements .
Determination
The pure methanol precipitation of milk protein in the sample after application of 9 - fluorenylmethyl chloroformate (FMOC) as a pre column derivatising agent, and of taurine in milk powder occurs, after a derivatization reaction, reverse - phase high performance liquid chromatography for the determination of content of The pure methanol precipitation of milk protein in the sample after application of 9 - fluorenylmethyl chloroformate (FMOC) as a pre column derivatising agent, and of taurine in milk powder occurs, after a derivatization reaction, reverse - phase high performance liquid chromatography for the determination of content of . methanol - 0.1 mol / L phosphate buffer as mobile phase (pH = 8. 0), a C18 column with a diode array detector (265)
Pharmaceutical Use
(1) liver - strengthening cholagogue effect: the experiment with guinea pigs showed that taurine could relieve bile obstruction, choleretic effect; (2) antipyretic and anti - inflammatory effect: by cns 5 - ht catecholamine system or system's role in reducing temperature; (3); (4) Anti - arrhythmia and cardiotonic effect; (5); hypoglycemic effect; (6) have a relaxation of taurine and antagonistic action of (1) liver - strengthening cholagogue effect: the experiment with guinea pigs showed that taurine could relieve bile obstruction, choleretic effect; (2) antipyretic and anti - inflammatory effect: by cns 5 - ht catecholamine system or system's role in reducing temperature; (3); (4) Anti - arrhythmia and cardiotonic effect; (5); hypoglycemic effect; (6) have a relaxation of taurine and antagonistic action of . myotonia myotonia.
Cosmetic Use
external research laboratory - confirmed by taurine - activating skin cells, and can provide young skin fast and lasting energy supplement with multiple protection external research laboratory - confirmed by taurine - activating skin cells, and can provide young skin fast and lasting energy supplement with multiple protection . taurine but also improve the immunity of the skin, resist the external environment on the skin of the invasion of the efficacy of the taurine but also improve the immunity of the skin, resist the external environment on the skin of the invasion of the efficacy of the .
Distribution And Metabolism
Taurine is widely distributed in animal tissues and cells within the marine animals, particularly, mammalian cells also contain high levels of taurine, especially the nerves, muscles and glands within the content is higher, is the most abundant free amino acid and taurine in almost all present in free form, and most of them in the cells, the intracellular and extracellular concentration ratio of 100 to 50000: 1, containing a total amount of the taurine in the range of about 12 to about 18 grams, wherein 15 - 93.18 present in blood plasma, 75% or more is present in the muscle,cardiomyocytes with serum taurine concentration ratio of 200: 1 body from dietary uptake or synthesis of taurine in itself, the animal food is a major source of dietary taurine, especially marine animals body from dietary uptake or synthesis of taurine in itself, the animal food is a major source of dietary taurine, especially marine animals .
vivo synthesis from sulphur - containing amino acid (cysteine, methionine, etc.) through a series of enzymatic reactions, is itself but a lower synthetic capacity taurine of relatively small molecular weight (125) daltons, no antigenicity, in a variety of administration routes are easy to be absorbed mainly from taurine of relatively small molecular weight (125) daltons, no antigenicity, in a variety of administration routes are easy to be absorbed mainly from .
taurine excretion by the kidneys, the kidney is regulated according to dietary taurine content in the discharge amount thereof, the content of taurine in vivo to maintain relatively stable taurine excretion by the kidneys, the kidney is regulated according to dietary taurine content in the discharge amount thereof, the content of taurine in vivo to maintain relatively stable .
Application And Development Prospect
Taurine has many physiological functions, is the essential nutrients for our Taurine has many physiological functions, is the essential nutrients for our . taurine is mainly used for medicine, food science, nutrition, although as has been acknowledged by our countrymen and accepted, but the imports are very small in terms of taurine amount taurine is mainly used for medicine, food science, nutrition, although as has been acknowledged by our countrymen and accepted, but the imports are very small in terms of taurine amount .
the world major countries and the annual per capita consumption of taurine amount roughly as: Japan, US, British 34g, Germany 32g, Canada 29g, France 26g, Korean 19g, Indonesia 17g, 17g, and Singapore, while China is less than 0. 2g taurine as a kind of health - care product is quite safe, taking some of the taurine - containing products, for health,Or it's desirable for taurine as a kind of health - care product is quite safe, taking some of the taurine - containing products, for health,Or it's desirable for .
taurine as a kind of high - quality nutrients, should better use taurine as a kind of high - quality nutrients, should better use . people like animals, will know that the cat's eyes always thatbright bright people like animals, will know that the cat's eyes always thatbright bright .
; and, in truth, and cat food of the duty by ; and, in truth, and cat food of the duty by . known, cats were favourite food is fish and mice known, cats were favourite food is fish and mice .
by expert studies show that in animals contain taurine, wherein the most abundant fish, shellfish and by expert studies show that in animals contain taurine, wherein the most abundant fish, shellfish and . at the same time, the mice formed contains a large amount of taurine at the same time, the mice formed contains a large amount of taurine .
through the diet, supplemented with a large number of cats, taurine, while the effect of taurine on the cat - sensitive cells in the retina after 2 h,So the cat's eyes can always thatbright bright through the diet, supplemented with a large number of cats, taurine, while the effect of taurine on the cat - sensitive cells in the retina after 2 h,So the cat's eyes can always thatbright bright . In a separate experiment, that do not contain taurine of cat food to feed the kittens, the cat's retina disease, blindness and even fully In a separate experiment, that do not contain taurine of cat food to feed the kittens, the cat's retina disease, blindness and even fully .
is felid not only, in the retina of the human eye also has a large amount of taurine, and therefore the effect of the taurine to the human eye is also vital to is felid not only, in the retina of the human eye also has a large amount of taurine, and therefore the effect of the taurine to the human eye is also vital to . the cornea of the eye and you have the ability to complex, i.e. when certain injuries to the cornea, can repair themselves
eyedrops in mostly contains taurine, is to strengthen the cornea's ability to repair itself, and fights blindness eyedrops in mostly contains taurine, is to strengthen the cornea's ability to repair itself, and fights blindness . can be seen, a daily supplement of taurine can enhance the cornea of the eye's ability to repair itself, can be seen, a daily supplement of taurine can enhance the cornea of the eye's ability to repair itself,.
taurine ophthalmic disease prevention is a special kind of amino acids, are essential for nutrients, and also has a healthy balance of vitamins taurine ophthalmic disease prevention is a special kind of amino acids, are essential for nutrients, and also has a healthy balance of vitamins . except for the eyes, taurine also have important effects on other organ for the record or field.
For example, study after confirmation, of a human heart. The highest content of taurine in taurine are to protect the myocardium, enhance cardiac function in cardiac myocytes of are to protect the myocardium, enhance cardiac function in cardiac myocytes of .
when the amount of inflow of calcium ions is high, the CHD, when the amount of inflow of calcium ions is high, the CHD, . taurine can adjust the amount of calcium ion in myocardial cells, maintain its equilibrium, achieving cardiotonic taurine can adjust the amount of calcium ion in myocardial cells, maintain its equilibrium, achieving cardiotonic .
taurine with the heart of the relationship is becoming the important study subject of the taurine with the heart of the relationship is becoming the important study subject of the . In addition, the effect of taurine on the lung,Liver, stomach intestine and has a protective effect in the aspect of daily life In addition, the effect of taurine on the lung,Liver, stomach intestine and has a protective effect in the aspect of daily life .
, taurine is most significant is the effect of enhancing immunity and resisting fatigue , taurine is most significant is the effect of enhancing immunity and resisting fatigue . taurine can bind leukocyte in generating hypochlorous acid and toxic substances, reduce the destruction of the leukocytes, so as to improve the body's immunity while taurine can bind leukocyte in generating hypochlorous acid and toxic substances, reduce the destruction of the leukocytes, so as to improve the body's immunity while .
, taurine can maintain the heart function, so that the normalization of blood circulation, eliminate fatigue so that the organism can effectively generate the energy for maintaining the constant , taurine can maintain the heart function, so that the normalization of blood circulation, eliminate fatigue so that the organism can effectively generate the energy for maintaining the constant . of taurine, can effectively eliminate the fatigue, it is the important factors influencing the health of of taurine, can effectively eliminate the fatigue, it is the important factors influencing the health of .
For cats, taurine is an essential amino acid,But the cat not synthesized taurine, and depend on the outside with a different uptake For cats, taurine is an essential amino acid,But the cat not synthesized taurine, and depend on the outside with a different uptake . feline, human liver protein synthesis are amino acids of the venue feline, human liver protein synthesis are amino acids of the venue .
but, in vivo synthesis of taurine and often failed to meet the body's physiological needs, but also often by uptake from the outside of the channel of replenish amino acids but, in vivo synthesis of taurine and often failed to meet the body's physiological needs, but also often by uptake from the outside of the channel of replenish amino acids . therefore, for the people, the therefore, for the people, the .
Taurine is a conditionally essential amino acids and the cats, people through food can supplement taurine Taurine is a conditionally essential amino acids and the cats, people through food can supplement taurine ., as previously described, in all of the food, the fish and shellfish in the taurine content is the most abundant , as previously described, in all of the food, the fish and shellfish in the taurine content is the most abundant .
often eat such food can supplement taurine, maintain health often eat such food can supplement taurine, maintain health .
Characterization Map
Taurine NMR (1HNMR).
