承擔課題
[1]國家自然科學基金（81172893）（主持）：PD-1/PD-L1拮抗肽的篩選及其在腫瘤免疫治療中的套用研究，起止年月：2012年～2015年
[2]國家自然科學基金（30901362）（主持）：乳腺癌PIWIL2、PLAC1抗原HLA-A2限制性CTL表位鑑定以及綜合改造策略研究，起止年月：2010年～2012年
[3]“十二五”科技部“重大新藥創製”重大專項（2012ZX09103301-023）（第2參與人）：新型抗腫瘤多肽藥物AS16的研製，起止年月：2012年～2015年
[4]河南省青年骨幹教師資助計畫（2010GGJS-016）（主持）：靶向細胞周期的新型抗腫瘤多肽的設計與活性研究，起止年月：2010年～2012年
[5]河南省基礎與前沿技術項目（102300410077）（主持）：基於MazEF複合物的新型抗菌肽的設計、合成與構效關係研究，起止年月：2010年～2012年
[6]河南省醫學科技攻關項目（201003008）（主持）：基於CT抗原的乳腺癌HLA-A2限制性抗原肽表位的篩選，起止年月：2010年～2012年
[7]河南省科技攻關項目（072102330038）（主持）：新型腫瘤抗原肽和廣譜疫苗的研究，起止年月：2007年～2009年
[8]河南省醫學科技攻關項目（200703013）（主持）：基於Cyclin/CDK複合物結構的抗腫瘤多肽的研究，起止年月：2007年～2009年
[9]教育部大學生創新實驗項目（101045910）（指導教師）：群體感應信號分子EDF在大腸桿菌中的作用靶點和信號轉導途徑的研究. 起止年月：2010年～2011年
[10]鄭州大學引進人才啟動基金項目（主持），起止年月：2006年～2008年
[11]河南省重點科技攻關項目（112102310068）（第2參與人）：結核耐藥相關抗原CTL表位的篩選與疫苗研製. 起止年月：2011年～2013年
[12]河南省傑出人才計畫項目（084200510014）（第2參與人）：結核病抗原肽表位的篩選和多價疫苗的研製. 起止年月：2008年～2010年
個人成就
主要論文
[1] Shi RR, Liu J, Zou Z, Qi YM, Zhai MX, Zhai WJ,Gao YF*. The immunogenicity of a novel cytotoxic T lymphocyte epitope from tumor antigen PL2L60 could be enhanced by 4-chlorophenylalanine substitution at position 1. Cancer Immunol Immunother. 2013, 62(11):1723-32.
[2] Haili Wang, Xi Chen, Yanping Chen, Lei Sun, Guodong Li, Mingxia Zhai, Wenjie Zhai, Qiaozhen Kang, Yanfeng Gao*, Yuanming Qi*. Antitumor activity of novel chimeric peptides derived from cyclinD/CDK4 and the protein transduction domain 4. Amino Acids, 2013, 44(2):499-510.
[3] Ji Z, Shi X, Liu X, Shi Y, Zhou Q, Liu X, Li L, Ji X, Gao Y, Qi Y, Kang Q. The membrane-cytoskeletal protein 4.1N is involved in the process of cell adhesion, migration and invasion of breast cancer cells. Exp Ther Med. 2012, 4(4):736-740.
[4] Yahong Wu, Yanfeng Gao*, Yujie He, Ranran Shi, Mingxia Zhai, Zongyin Wu, Meng Sun, Wenjie Zhai, Xi Chen, Yuanming Qi*. A novel cytotoxic T lymphocyte epitope analogue with enhanced activity derived from cyclooxygenase-2. Scand J Immunol. 2012, 76(3):278-85.
[5] Chao Dai, Zhaoqing Xu*, Fei Huang, Zhengkun Yu*, Yan-Feng Gao *. Lewis Acid-Catalyzed, Copper(II)-Mediated Synthesis of Heteroaryl Thioethers under Base Free Conditions. Journal of Organic Chemistry. 2012, 77(9):4414-9.
[6] Fei Chen, Mingxia Zhai, Yuhuang Zhu, Yuanming Qi, Wenjie Zhai, Yanfeng Gao *. In vitro and in vivo identification of a novel cytotoxic T lymphocyte epitope from Rv3425 of Mycobacterium tuberculosis. Microbiology and Immunology. 2012, 56(8):548-53.
[7] Zongyin Wu, Yanfeng Gao*, Yahong Wu, Wei Liu, Meng Sun, Mingxia Zhai, Yuanming Qi, Yong Ye. Identification of a novel CD8+ T cell epitope derived from cancer-testis antigen MAGE-4 in oesophageal carcinoma. Scand J Immunol. 2011, 74:561-567.
[8] Wei Liu, Mingxia Zhai, Zongyin Wu, Yuanming Qi, Yahong Wu, Chao Dai, Meng Sun, Lu Li, Yanfeng Gao* Identification of a novel HLA-A2-restricted cytotoxic T lymphocyte epitope from cancer-testis antigen PLAC1 in breast cancer . Amino Acids. 2012, 42(6):2257-65.
[9] Yu-huang Zhu, Yan-feng Gao*, Fei Chen, Wei Liu, Ming-xia Zhai, Wen-jie Zhai, Yuan-ming Qi*, Yong Ye. Identification of novel T cell epitopes from efflux pumps of Mycobacterium tuberculosis. Immunology Letters. 2011, 140(1-2):68-73.
[10] Jun-wei Zhao, Zhan-qiang Sun, Hong-guo Yang, Chong-zhen Zhang and Xiao-li Yu, Zi-lu Wen, Yan-feng Gao, Xiao-kui Guo, Yuan-ming Qi, Shu-lin Zhang. Cloning, expression and immunological evaluation of a short fragment from Rv3391 of Mycobacterium tuberculosis. Annals of Microbiology, 2011, 61(2):345-353.
[11] Hong Lv, Yan-feng Gao*, Ya-hong Wu, Ming-xia Zhai, Lu Li, Yu-huang Zhu, Wei Liu, Zong-yin Wu, Fei Chen, Yuan-ming Qi. Identification of a novel cytotoxic T lymphocyte epitope from CFP21, a secreted protein of Mycobacterium tuberculosis. Immunology Letters. 2010, 133(2):94-8.
[12] Yanfeng Gao*, Kun Chen, Bin Zhang, Xiang Li, Lu Chen, Yuanjun Li, Xiangjiao Jia, Yongxing Lei, Zhongyi Yan, Liangliang Kong, Nan Wang, Wei Liu, Yuanming Qi. Antioxidant and free radical scavenging activity of the extracellular death factor in Escherichia coli. Peptides, 2010, 31(10):1821-1825.
[13] Dong-dong Wu, Yan-feng Gao, Li-xiang Chen*, Yuan-ming Qi*, Qiao-zhen Kang, Hai-li Wang, Lin-yu Zhu, Yong Ye, Ming-xia Zhai. Anti-tumor effects of a novel chimeric peptide on S180 and H22 xenografts bearing nude mice. Peptides. 2010, 31(5):850-864.
[14] Yan-feng Gao, Zhan-qiang Sun, Feng Qi, Yuan-ming Qi*, Ming-xia Zhai, Hui-ping Lou, Li-xiang Chen, Yong-xin Li, Xian-yuan Wang. Identification of a new broad-spectrum CD8 T cell epitope from over-expressed antigen COX-2 in esophageal carcinoma. Cancer Letters, 2009, 284(1):55-61.
[15] Hui-ping Lou, Yan-feng Gao*, Ming-xia Zhai, Yuan-ming Qi*, Li-xiang Chen, Hong Lv, Ji-bing Yu, Yong-xin Li. A novel peptide from α5 helix of Asterina pectinifera cyclin B conjugated to HIV-Tat with cytotoxic and apoptotic effects against human cancer cells. Bioorganic & Medicinal Chemistry Letters, 2008, 18(16):4633-4637.
[16] Yan-feng Gao, Ming-xia Zhai, Wei-xia Liu, Xin Liu, Ye Yuan, Yuan-ming Qi*, Rui Wang*. Structure-activity relationships of the dimeric analogues of endomorphin-2 with different lengths of spacers. Protein & Peptide Letters, 2008, 15(3):275-279.
[17] Chang-Feng Hu, Chuan-Jun Zhu, Yan-Feng Gao, Xuan Shao, Rui Wang, Yu-Xin Cui. Characteristic Fragmentation Behavior of the Analogs of endomorphin-2 with Phenylglycine in Position 3 or 4 by ESI-FT Tandem Mass Spectrometry. Chinese Chemical Letters. 2008, 19(12):1479-1482.
[18] Yun Feng, Ying-wei Lu, Xin Lin, Yan-feng Gao, Qian-yu Zhao, Wei Li, Rui Wang*. Endomorphins and morphine limit anoxia-reoxygenation-induced brain mitochondrial dysfunction in the mouse. Life Sciences, 2008, 82(13-14):752-763.
[19] Xuan Shao, Yan-feng Gao, Chuan-jun Zhu, Xue-hui Liu, Jin-long Yao, Yu-xin Cui, Rui Wang*. Conformational analysis of endomorphin-2 analogs with phenylalanine mimics by NMR and molecular modeling. Bioorganic & Medicinal Chemistry, 2007, 15(10): 3539-3547.
[20] Li-xiang Chen, Yuan-ming Qi*, Rui Wang*, Xin Duan, Yan-feng Gao, Ding-jian Yang. Roles of nitric oxide synthase inhibitor on antinociceptive effects of μ-Opioid agonist in mice. Protein & Peptide Letters, 2006, 13(10): 993-997.
[21] Yan-feng Gao, Xin Liu, Wei-xia Liu, Yuan-ming Qi, Xue-feng Liu, Rui Wang*. Opioid receptor binding and antinociceptive activity of the analogues of endomorphin-2 and morphiceptin with phenylalanine mimics in the position 3 or 4. Bioorganic & Medicinal Chemistry Letters, 2006, 16(14):3688-3692.
[22] Yan-feng Gao, Xin Liu, Jie Wei, Bei-bei Zhu, Qiang Chen, Rui Wang*. Structure-activity relationship of the novel bivalent and C-terminal modified analogues of endomorphin-2. Bioorganic & Medicinal Chemistry Letters, 2005, 15(7):1847-1850.
[23] Jie Wei, Xuan Shao, Mao-zhen Gong, Bei-bei Zhu, Yu-xin Cui, Yan-feng Gao, Rui Wang*. Structure-activity relationships of novel endomorphin-2 analogues with N-O turns induced by α-aminoxy acids. Bioorganic & Medicinal Chemistry Letters, 2005, 15(12):2986-2989.
[24] Yi-feng Zhou, Rui Wang*, Zhao-qing Xu, Wen-jin Yan, Lei Liu, Yan-feng Gao, Chao-shan Da. Boc-L-proline as a new chiral ligand for enantioselective phenylacetylene addition to aromatic aldehydes. Tetrahedron: Asymmetry. 2004, 15(4):589-591.
[25] Xin Lin, Ding-jian Yang, Wen-qing Cai, Qian-yu Zhao, Yan-feng Gao, Qiang Chen, Rui Wang*. Endomorphins, endogenous opioid peptides, provide antioxidant defense in the brain against free radical-induced damage. Biochimica et Biophysica Acta (BBA) - Molecular Basis of Disease, 2003, 1639(3):195-202.
獲得專利
[1] PLAC1抗腫瘤CTL表位肽及其套用. 專利授權號：ZL 201010205684.0
[2] PIWIL2抗腫瘤CTL表位肽及其套用. 專利授權號：ZL201010276917.6
[3] 抗結核CTL表位肽. 專利授權號：ZL 200910065403.3.
[4] MAGE-4抗腫瘤CTL表位肽及其套用. 專利授權號：ZL201010212512.5
[5] 結核分枝桿菌抗原限制性表位肽. 專利授權號：201010030225.3.
[6] 結核耐藥相關外排蛋白來源抗結核CTL表位肽及其套用. 專利授權號：201110138646.2
[7] MTA1來源的抗腫瘤CTL表位肽及其套用. 專利授權號：201110138635.4
[8] PL2L60來源的CTL表位肽及其套用. 專利授權號：201210001557.8
編寫教材
[1]生物化學實驗原理與技術（普通高等教育“十二五”規劃教材），化學工業出版社，2010，副主編
[2]動物生物學與生理學實驗指導，鄭州大學出版社，2008年，副主編
榮譽
[1]第十一屆中國國際多肽會“Yong Peptide Scientist Award”
[2]“沈瓊食管癌研究獎勵基金”優秀科研成果二等獎
[3]河南省優秀中青年骨幹教師
[4]河南省教學技能競賽二等獎
[5]河南省自然科學優秀論文一等獎2項
[6]河南省自然科學優秀論文二等獎4項
[7]鄭州大學校級青年骨幹教師
[8]連年被評為“學生最滿意教師”
[9]2009年鄭州大學生物工程系青年教師講課比賽三等獎
[10]2010和2011年度河南省優秀碩士學位論文，指導教師
[11]2007和2010年度鄭州大學“挑戰杯”活動特等獎，指導教師
[12]2009年鄭州大學校級優秀本科生畢業論文1人，指導教師
