人物介紹
陳國凱博士現任澳門大學健康科學學院副教授/助理院長（Assistant Dean, Faculty of Health Sciences, University of Macau），國家青年千人計畫專家。四川大學生物化學學士（Sichuan University），美國貝勒醫學院博士（Baylor College of Medicine），威斯康星大學博士後/助理科學家（Assistant Scientist, Wisconsin Genome Center, University of Wisconsin, Madison.Morgridge Institute of Discovery），美國公立衛生院誘導多能幹細胞中心主任（Director, iPSC and Genome Engineering Core Facility, National Heart, Lung and Blood Institute, National Institutes of Health, USA）。陳國凱博士師從人胚胎幹細胞之父James Thomson，開發了當前國際流行的以E8培養基為基礎的幹細胞培養體系，為人類胚胎幹細胞和誘導多能幹細胞的技術發展作出了突出貢獻，開發了新的細胞培養和操作手段，並發明了多種幹細胞培養分化體系。他的成果正被幹細胞領域廣泛的套用,一些專利發明已進入商業運作成為行業標準。目前團隊在以下領域開展工作：研究幹細胞生長發育的分子機理；開發與臨床套用相匹配的幹細胞生長發育條件；建立幹細胞移植的動物模型；研發乾細胞大規模產業化的生產條件和流程。
部分論文
Liu W, Ren Z, Lu K, Song C, Cheung ECW, Zhou Z, Chen G. The Suppression of Medium Acidosis Improves the Maintenance and Differentiation of Human Pluripotent Stem Cells at High Density in Defined Cell Culture Medium. Int J Biol Sci. 2018 Apr 5;14(5):485-496.
Lin Y, Linask KL, Mallon B, Johnson K, Klein M, Beers J, Xie W, Du Y, Liu C, Lai Y, Zou J, Haigney M, Yang H, Rao M, Chen G. Heparin Promotes Cardiac Differentiation of Human Pluripotent Stem Cells in Chemically Defined Albumin-Free Medium, Enabling Consistent Manufacture of Cardiomyocytes. Stem Cells Transl Med. 2017 Feb;6(2):527-538.
Lin Y, Li XY, Willis AL, Liu C, Chen G, and Weiss S. Snail1-Dependent Control of Embryonic Stem Cell
Pluripotency and Lineage Commitment. Nature Communication, 2013.
Beers J, Gulbranson DR, George N, Siniscalchi LI, Jones J, Thomson JA and Chen G. Passaging and colony
expansion of human pluripotent stem cells by enzyme-free dissociation in chemically defined culture conditions. Nature Protocols, 2012.
Chen G, Gulbranson DR, Yu P, Hou Z, Thomson JA. Thermal stability of fibroblast growth factor protein is a
determinant factor in regulating self-renewal, differentiation, and reprogramming in human pluripotent stem
cells. Stem Cells, 2012.
Chen G, Gulbranson DR, Hou Z, Bolin JM, Ruotti V, Probasco MD, Smuga-Otto K, Howden SE, Diol NR, Propson NE, Wagner R, Lee GO, Antosiewicz-Bourget J, Teng JMC and Thomson JA. Chemically defined conditions for human iPS cell derivation and culture. Nature Methods, 2011.
Chen G, Hou Z, Gulbranson DR and Thomson JA. Actin-Myosin Contractility Is Responsible for the Reduced
Viability of Dissociated Human Embryonic Stem Cells. Cell Stem Cell, 2010.
Chen G, Zhuchenko O and Kuspa A. Immune-like phagocyte activity in the social amoeba. Science. 2007.
發明專利
Chen G, Thomson JA. Simplified basic media for human pluripotent cell culture.
Chen G, Hou Z, Thomson JA. Improved vitronectin variants improve culture capacity supporting self-renewal or differentiation in human pluripotent stem cells.
Chen G, Thomson JA. Stabilized FGF1 mutants improve culture capacity supporting self-renewal or differentiation in human pluripotent stem cells.
Chen G, Hou Z, Thomson JA. Stabilized FGF2 mutant improves culture capacity supporting self-renewal or differentiation in human pluripotent stem cells.
Chen G, Lin Y. Improvement of chemically defined culture for hPSCs. (Pending)
Chen G, Lin Y. Cardiomyocyte differentiation from hPSCs in defined conditions. (Pending)
