錢偉強[北京大學生命科學研究院研究員]

錢偉強：北京大學生命科學研究院研究員，青年千人引進人才。主要從事DNA去甲基化途徑和轉座子對附近基因的表達調控研究 。

個人經歷

1998 - 2002 ， 理學學士 , 生物學 , 北京師範大學

2002 - 2007，理學博士 , 遺傳學 , 中國科學院遺傳與發育生物學研究所

2007 - 2009， 博士後 , 遺傳學 , 美國康奈爾大學

2009 - 2010 ，博士後 , 遺傳學 , 美國加州大學河濱分校

2011 - 2011， 博士後 , 遺傳學 , 美國普渡大學

2012 - 2013，青年研究員 , 中國科學院上海植物逆境生物學研究中心

2013 - 至今， 研究員 , 北京大學生命科學學院

研究領域

研究背景和方向

以擬南芥為模式系統，研究表觀遺傳學信息如何影響基因表達以及植物生長發育

主要研究方向

DNA去甲基化途徑和轉座子對附近基因的表達調控

具體研究內容

DNA甲基化修飾是一種重要的表觀遺傳學標誌，在基因的表達調控，基因組的結構及穩定性，X染色體的失活和其它生長發育調控等方面都具有十分重要的作用。DNA甲基化的水平主要由甲基化和去甲基化這兩個途經來協同調控。在植物體內，DNA去甲基化主要是由repressor of silencing 1 (ROS1)基因家族介導的鹼基切除修復機制來實現。在植物中對DNA甲基化途經的研究已經比較深入，但是對DNA去甲基化的分子機制還不清楚。運用遺傳學，生物化學和細胞生物學的方法，對植物體內DNA去甲基化的酶學機制以及調控去甲基化的途經進行深入的研究。

參考資料

發表論文

Li Y*, Córdoba-Cañero D*, Qian W*, Zhu X, Tang K, Ariza, R Arjona T and Zhu JK , An AP endonuclease functions in active DNA demethylation and gene imprinting in Arabidopsis , PLoS Genet , 2015 , 11(1): e1004905. doi:10.1371/journal.pgen.1004905 (*Contributed equally to this work)

Duan C, Zhang H, Tang K, Zhu X, Qian W, Hou Y, Wang B, Lang Z, Zhao Y, Wang X, Wang P, Zhou J, Liang G, Liu N, Wang C, Zhu JK , Specific but interdependent functions for Arabidopsis AGO4 and AGO6 in RNA-directed DNA methylation , EMBO J , 2014 , pii: e201489453

Qian W*, Miki D*, Lei M*, Zhu X, Zhang H, Liu Y, Li Y, Lang Z, Wang J, Tang K, Liu R and Zhu JK. , Regulation of Active DNA Demethylation by an α-Crystallin Domain Protein in Arabidopsis , Mol Cell , 2014 , 55(3):361-71(*Contributed equally to this work)

Zhang H, Tang K, Qian W, Duan CG, Wang B, Zhang H, Wang P, Zhu X, Lang Z, Yang Y and Zhu JK , An Rrp6-like protein positively regulates noncoding RNA levels and DNA methylation in Arabidopsis , Mol Cell , 2014 , 8;54(3):418-30

Li X, Qian W, Zhao Y, Wang C, Shen J, Zhu J-K and Gong Z , The anti-Silencing role of the RNA-directed DNA methylation pathway and a histone acetyltransferase in Arabidopsis , Proc Natl Acad Sci U S A , 2012 , 109(28):11425-30

Qian W*, Miki D*, Zhang H, Liu Y, Zhang X, Tang K, Kan Y, La H, Li X, Li S, Zhu X, Shi X, Zhang K, Pontes O, Chen X, Liu R, Gong Z and Zhu JK , A Histone Acetyltransferase Regulates Active DNA Demethylation in Arabidopsis , Science , 2012 , 336(6087):1445-8

Mang HG*, Qian W*, Zhu Y, Qian J, Kang HG, F.Klessig D and Hua J , ABA deficiency antagonizes high temperature inhibition of disease resistance through enhancing nuclear accumulation of resistance proteins SNC1 and RPS4 in Arabidopsis , Plant Cell , 2012 , 24 (3): 1271-84,*Contributed equally to this work

Martínez-Macías M*, Qian W*, Miki D*, Pontes O, Liu Y, Tang K, Liu R, Morales-Ruiz T, R. Ariza R, Roldán-Arjona R, and Zhu JK , A DNA 3’-phosphatase functions in active DNA demethylation in Arabidopsis , Mol Cell , 2012 , 45(3): 357-70,*Contributed equally to this work

Wang L*, Li Z*, Qian W, Guo W, Gao X, Huang L, Wang H, Zhu H, Wu JW, Wang D and Liu D , Arabidopsis purple acid phosphatase AtPAP10 is predominantly associated with the root surface and play an important role in plant tolerance to phosphate limitation , Plant Physiol , 2011 , 157 (3): 1283-99

Tran HT, Qian W, Hurley BA, She YM, Wang D, and Plaxton WC , Biochemical and molecular characterization of AtPAP12 and AtPAP26: the predominant purple acid phosphatase isozymes secreted by phosphate-starved Arabidopsis thaliana , Plant Cell Environ , 2010 , 33 (11): 1789-803

Gao Z, Liu HL, Daxinger L, Pontes O, He X, Qian W, Lin H, Xie M, Lorkovic ZJ, Zhang S, Miki D, Zhan X, Pontier D, Lagrange T, Jin H, Matzke AJ, Matzke M, Pikaard CS and Zhu JK , An RNA polymerase II- and AGO4-associated protein acts in RNA-directed DNA methylation , Nature , 2010 , 6;465(7294):106-9

Zhu Y, Qian W, and Hua J , Temperature modulates plant defense responses through NB-LRR proteins , PLoS Pathog , 2010 , 1;6 (4):e1000844

Qin C, Qian W, Wang W, Wu Y, Yu C, Jiang X, Wang D, Wu P , GDP-mannosepyrophosphorylase is a genetic determinant of ammonium sensitivity in Arabidopsis thaliana , Proc Natl Acad Sci USA , 2008 , 25;105(47):18308-13

Liu X*，Qian W*, Liu X, Qin H, and Wang D , Molecular and functional analysis of hypoxanthine-guanine phosphoribosyltransferase from Arabidopsis thaliana , New Phytol , 2007 , 175(3):448-61,* Contributed equally to this work

Qian W, Yu C, Qin H, Liu X, Zhang A, Johansen, I.E, and Wang D , Molecular and functional analysis of phosphomannomutase (PMM) from higher plants and genetic evidence for the involvement of PMM in ascorbic acid biosynthesis in Arabidopsis and Nicotiana benthamiana , Plant J , 2007 , 49(3):399-413

參考文獻