![王天文[華東理工大學副教授]](/img/b/f23/nBnauM3XwYjMzQzM0YzM2EDM2UTM1QDN5MjM5ADMwAjMwUzL2MzL0UzLt92YucmbvRWdo5Cd0FmL0E2LvoDc0RHa.jpg "王天文[華東理工大學副教授]")
人物簡介
2000年畢業於河南農業大學，獲得微生物學學士學位，2003年畢業於福建農業大學生物化學與分子生物學專業，獲碩士學位，2006年畢業於華東理工大學生物化工專業，獲博士學位。隨後分別在清華大學和新加坡國立大學（The National University of Singapore）進行博士後（Research fellow）研究。曾任職於天津大學和江南大學， 2013年加入華東理工大學生物工程學院。AFOB會員，中國生物工程學會會員，上海生物工程學會會員。至2014年4月，已發表SCI論文19篇，申請發明專利多項，其中美國專利一項。
教育經歷
2003-2006 華東理工大學生物化工 博士
2000-2003 福建農業大學 生物化學與分子生物學 碩士
1996-2000 河南農業大學 微生物學 學士
研究與工作經歷
2007-2008 清華大學生物科學與技術系 博士後
2009-2012.4 江南大學生物工程學院 教師
2011-2012 新加坡國立大學化學與生物分子工程系 博士後（Research fellow）
2012年9月至今 華東理工大學生物反應器工程國家重點實驗室 副教授
研究方向
1.生物化學
主要針對具有重要套用前景的酶、蛋白等。綜合運用生物化學和分子生物學的手段，發現和改造具有重要套用前景的酶或蛋白，並套用於生物催化中。
2.合成生物學
以生產具有抗癌作用的天然產物（如抗生素、非核糖體肽等）為目標產物，運用代謝工程手段和合成生物學策略，構建理性化設代謝途徑，通過發酵等過程，獲得目標產物。
3.RNA生物學
主要針對蛋白質合成機器核糖體的結構和功能，設計合適的tRNA，實現在蛋白質中摻入利於後期修飾的非天然胺基酸，為製備超高穩定性酶催化劑或藥物蛋白提供解決方案。
主要科研項目
1. 存活素納米抗體(Survivin-Nb)工程化酵母製備與抗肺癌及其幹細胞活性研究, 上海市人才計畫項目（“浦江人才”項目，F200-2R-13110，2013.09.01-2015.8.31），參與人
2. L-苯丙氨酸的代謝酶空間組織工程研究，國家自然科學基金（青年基金，3100054，2011.1-2013.12），負責人
3. 氧化脅迫產朊假絲酵母醇脫氫酶的谷胱甘肽化研究（啟動項目，2009-1011），負責人
4. 利用RED重組和Kan/sacB選擇標籤構建L-苯丙氨酸生產菌株，教育部高校科研基本業務費（青年探索基金，JUSRP10917，2009-2011），負責人
5. 基於ELP-Intein體系的新型非融合重組蛋白規模化表達純化技術（教育部重點實驗室開放課題，KLIB-KF201004，2011-2012），負責人
學術文章
2014
F. Ma, T.Wang, X.Ma, and P.Wang, Identification and Characterization of the Protein Encoded by ORF_382 as an L-Threonine Dehydrogenase, Journal of Microbiology and Biotechnology, Accepted.
2013
L.Shi, L.Tang, X.Ma, T.Wang, P. Wang, Efficient Preparation of an Anti-tumor Protein TmSm by Reversible Phase Transition of ELPs and Self-cleaving Function of Intein, China Biotechnol., 2013, 33(10):89-95.[In CHS]
C.Zhang, J.Zhang, Z.Kang, G.Du, X.Yu, T.Wang,J.Chen,Enhanced production of L-phenylalanine in Corynebacterium glutamicum due to the introduction of Escherichia coli wild-type gene aroH, J. Ind. Microbiol. Biotech., 2013, 40(6):643-651. PMID:23526182.
N. Lin, W. Zheng, L. Li, H. Liu, T. Wang, P. Wang, X. Ma*, A novel system enhancing the endosomal escapes of peptides promotes Bak BH3 peptide inducing apoptosis in lung cancer A549 cells. Targeted Oncology. 2013. DOI: 10.1007/s11523-013-0282-9,
2012
T. Wang, A. Li, G. Du and J. Chen, A simple and universal method to express protein in its unfused form, Protein Pept. Lett., 2012, 19 (9):930-933. PMID:22316309.
T. Wang, X. Ma, G. Du and J. Chen, Overview of regulatory strategies and molecular elements in metabolic engineering, Mol. Biotechnol., 2012,52(3):300-388. PMID: 22359157.
T. Wang, X. Ma, H.Zhu, A. Li, G. Du and J. Chen, Available methods for assembling expression cassettes for synthetic biology, Appl. Microbiol. Biotechnol., 2012, 93(5):1853-1863. PMID:22311648.
Qiu, XF, Wang, TW, Zhong, XM, Du, GC, Chen, J , Screening and characterization of an aerobic nitrifying-denitrifying bacterium from activated sludge, Biotechnol. Bioproc. Eng., 2012, 17(2):353-360.
Xu Y, Zheng W, Wang T, Wang P, Zhu L, Ma X, Genetic protein TmSm(T34A) enhances sensitivity of chemotherapy to breast cancer cell lines as a synergistic drug to doxorubicin, Biomed. Pharmacother., 2012, 66(5):368-372.
2011
H. Zhou, X. Liao, L. Liu, T. Wang*, G. Du and J. Chen, Enhanced L-phenylalanine production by recombinant Escherichia coli BR-42 (pAP-B03) resistant to bacteriophage BP-1 via a two-stage feeding approach, J Ind. Microbiol. Biotechnol., 2011, 38(9):1219-1227. [Co-corresponding author], PMID:21104105.
2010
T. Wang, Y. Hu, X. Liao, G. Du, J. Chen and J. Xu, Anti-oxidative stress and beyond: multiple functions of the protein glutathionylation, Protein Pept. Lett., 2010, 17(10):1234-1244. [Co-1st and -corresponding author], PMID:20394582.
H. Zhou, X. Liao, T. Wang, G. Du and J. Chen, Enhanced l-phenylalanine biosynthesis by co-expression of pheA(fbr) and aroF(wt), Bioresour. Technol., 2010, 101(11):4151-4156. [Co-corresponding author], PMID:20137911.
2009
T. Wang,A. Qiu, F. Meng and H. Zhou, Changing the Metal Binding Specificity of Superoxide Dismutase from Thermus thermophilus HB-27 by a Single Mutation, Mol. Biotechnol., 2009, 42(2):146-153. PMID:19191037.
2006
T. Wang, H. Zhu, X. Ma, Z. Fei, Y. Ma and D. Wei, Enhancing enzymatic activity of penicillin G acylase by coexpressing pcm gene, Appl. Microbiol. Biotechnol., 2006, 72(5):953-958. PMID:16550378.
T. Wang, H. Zhu, X. Ma, Y. Ma and D. Wei, Structure-based stabilization of an enzyme: the case of penicillin acylase from Alcaligenes faecalis, Protein Pept. Lett., 2006, 13(2):177-183. PMID:16472081.
T. W. Wang, H. Zhu, X. Y. Ma, T. Zhang, Y. S. Ma and D. Z. Wei, Mutant library construction in directed molecular evolution: casting a wider net, Mol. Biotechnol., 2006,34(1):55-68. PMID:16943572.
H. Zhu, T. W. Wang, S. J. Sun, Y. L. Shen and D. Z. Wei, Chromosomal integration of the Vitreoscilla hemoglobin gene and its physiological actions in Tremella fuciformis, Appl. Microbiol. Biotechnol., 2006, 72(4):770-776. PMID:16501972.
X. Ma, W. Zheng, T. Wang, D. Wei and Y. Ma, Optimization and high-level expression of a functional GST-tagged rHLT-B in Escherichia coli and GM1 binding ability of purified rHLT-B, J Microbiol., 2006, 44(3):293-300. PMID:16820759.
X. Ma, W. Zheng and T. Wang, Leukocyte function-associated antigen-1: structure, function and application prospects, Protein Pept. Lett., 2006, 13(4):397-400. PMID:16712517.
H. Zhu, R. J. Pan, T. W. Wang, Y. L. Shen and D. Z. Wei, Functional solubilization of aggregation-prone TRAIL protein facilitated by coexpressing with protein isoaspartate methyltranferase, Appl. Microbiol. Biotechnol., 2006, 72(5):1033-1038. PMID:16575568.
X. Ma, W. Zheng, D. Wei, Y. Ma, T. Wang, J. Wang, Q. Liu and S. Yang, High-level expression, purification and pro-apoptosis activity of HIV-TAT-survivin (T34A) mutant to cancer cells in vitro, J Biotechnol., 2006, 123(3):367-378. PMID:16406157.
X. Ma, W. Zheng, D. Wei, Y. Ma, T. Wang,J. Wang, Q. Liu and S. Yang, Construction, expression, and purification of HIV-TAT-survivin (T34A) mutant: a pro-apoptosis protein in Escherichia coli, Protein Expr. Purif., 2006, 47(1):36-44. PMID: 16260148.
2005
W. Zheng, X. Ma, D. Wei, T. Wang, Y. Ma and S. Yang, Molecular cloning and bioinformatics analysis of a novel spliced variant of survivin from human breast cancer cells, DNA Seq., 2005,16(5):321-328. PMID:16329164.
發明專利
1. Zhi Li, Tianwen Wang, Jiandong Zhang, Preparation of enantiopure vicinal diols from racemic and meso-epoxides by tandem biocatalysis via enantioselective hydrolysis and oxidations, USApplication No.: 61/838,981
2. 王天文, 馬興元, 馬飛, 王平，一種ssDNA-蛋白質酶法交聯方法，公開號：CN103361321A.
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