![孫永華[中國科學院研究員]](/img/5/751/nBnauM3X4QjMwQjN4UzM0YTN1UTM1QDN5MjM5ADMwAjMwUzL1MzL4UzLt92YucmbvRWdo5Cd0FmLxE2LvoDc0RHa.jpg "孫永華[中國科學院研究員]")
研究領域
主要從事魚類卵質和早期發育的分子調控，以及魚類基因組操作和定向育種研究(Cell Research, 2000; 中國科學，2014)。
闡明Smad蛋白在早期胚胎中介導BMP信號通路的網路調控機制（Journal of Biological Chemistry, 2014），發現經典Wnt信號通路的新調控因子Lbx2並闡明其激活Wnt信號活性的分子機制(Nature Communications, 2014)；首獲轉基因魚的屬間克隆魚，揭示母源因子對克隆魚發育圖式形成的重要作用和克隆魚敗育的分子機制（Biology of Reproduction, 2005; Journal of Physiology, 2014）。建立不依賴於生長激素水平且快速生長的激活型生長激素受體轉基因魚模型（Transgenic Research, 2011, 中國發明專利授權，2013）；利用“合成生物學”概念研製首例具有從頭合成omega-6和omega-3不飽和脂肪酸能力的轉基因魚（Marine Biotechnology, 2014，中國發明專利授權，2016）；發現魚類早期胚胎中廣泛存在微同源末端介導的DNA雙鏈斷裂修復（MMEJ）機制（Mutation Research, 2015）,創製特異高效的魚類原始生殖細胞操作技術（Marine Biotechnology, 2013），並將之套用於穩定高效的魚類基因組操作（中國發明專利授權，2015）。在 Nature Communications、Journal of Biological Chemistry、Mutation Research、Biology of Reproduction、Cell Research、Journal of Physiology、PLoS Genetics、Marine Biotechnology、Transgenic Research以及《中國科學》等國內外學術刊物上發表論文60 余篇。多次作為會議主席或秘書長主持國際性或全國性學術會議，應邀10多次在國際學術會議上作口頭報告。主持科技部973 課題、國家自然科學基金優秀青年科學基金、重大研究計畫課題和面上項目等一批科研項目。
主持建立國家斑馬魚資源中心，將該中心建成國際學術界公認的全球三大斑馬魚資源庫之一。
人物經歷
1993－1997：武漢大學生命科學學院本科學習，獲學士學位
1997－2002：中國科學院水生生物研究所研究生學習，獲博士學位（期間獲中國科學院彭蔭剛獎學金一等獎及湖北省優秀博士學位論文獎）
2002－2004：中國科學院水生生物研究所助理研究員
2005－2006：法國國家科研中心分子細胞及遺傳學研究所（Institut de Génétique et de Biologie Moléculaire et Cellulaire， Centre National de la Recherche Scientifique）博士後及合作訪問研究
2006－今：法蘭西科學院和中國科學院聯合項目“法中科學及套用基金會（La Fondation Franco-Chinoise pour la Science et ses Applications， FFCSA）”博士後研究
2005－2010：中國科學院水生生物研究所副研究員
2010－：中國科學院水生生物研究所研究員，國家斑馬魚資源中心主任
主要代表性論文
1)Liu, L., Pan, L., Li, K., Zhang, Y., Zhu, Z. and Sun, Y.H.* (2016). Zebrafish Health Conditions in the China Zebrafish Resource Center and 20 Major Chinese Zebrafish Laboratories. Zebrafish 13 Suppl 1, S8-S18.
2)He, M.-D., Zhang, F.-H., Wang, H.-L., Wang, H.-P., Zhu, Z.-Y. and Sun, Y.-H.* (2015). Efficient ligase 3-dependent microhomology-mediated end joining repair of DNA double-strand breaks in zebrafish embryos. Mutation Research/Fundamental and Molecular Mechanisms of Mutagenesis 780, 86-96.
3)Pang, S.-C., Wang, H.-P., Zhu, Z.-Y. and Sun, Y.-H*. (2015). Transcriptional Activity and DNA Methylation Dynamics of the Gal4/UAS System in Zebrafish. Marine Biotechnology, 17(5):593-603.
4)Xie, X., Pan, L. and Sun, Y.* (2015). Growing with the world: rapid development of the zebrafish research in China and the China Zebrafish Resource Center. Sci China Life Sci 58, 396-399.
5)Ye, D., Zhu, Z. and Sun, Y.* (2015). Fish genome manipulation and directional breeding. Sci China Life Sci 58, 170-177.
6)Wei Z-Q, Xiong F, He M-D, Wang H-P, Zhu Z-Y, Sun Y-H*. (2015) Suppression of Ligase 4 or Xrcc6 activities enhances the DNA homologous recombination efficiency in zebrafish primordial germ cells. Acta Hydrobiol. 39(2):339-48.
7)Wei, C. Y., Wang, H. P., Zhu, Z. Y. and Sun, Y. H.* (2014). Transcriptional factors Smad1 and Smad9 act redundantly to mediate zebrafish ventral specification downstream of smad5. The Journal of Biological Chemistry 289, 6604-6618.
8) Sun, Y. H.* and Zhu, Z. Y. (2014). Cross-species cloning: influence of cytoplasmic factors on development. J Physiol 592, 2375-2379.
9)Lu, F.-I., Sun, Y.-H.*, Wei, C.-Y., Thisse, C. and Thisse, B. (2014). Tissue-specific derepression of TCF/LEF controls the activity of the Wnt/β-catenin pathway. Nat Commun 5, 5368. (*co-first author)
10)Pang, S. C., Wang, H. P., Li, K. Y., Zhu, Z. Y., Kang, J. X. and Sun, Y. H.* (2014). Double Transgenesis of Humanized fat1 and fat2 Genes Promotes Omega-3 Polyunsaturated Fatty Acids Synthesis in a Zebrafish Model. Mar Biotechnol (NY) 16, 580-593.
11)Xiong, F., Wei, Z. Q., Zhu, Z. Y. and Sun, Y. H.* (2013). Targeted Expression in Zebrafish Primordial Germ Cells by Cre/loxP and Gal4/UAS Systems. Mar Biotechnol (NY) 15, 526-539.
12)Liu, C.-W., Xiong, F., Jia, H.-Z., Wang, X.-L., Cheng, H., Sun, Y.-H.*, Zhang, X.-Z., Zhuo, R.-X. and Feng, J. (2013a). Graphene-Based Anticancer Nanosystem and Its Biosafety Evaluation Using a Zebrafish Model. Biomacromolecules 14, 358-366.
13)Wang, Y.-W., Wei, C.-Y., Dai, H.-P., Zhu, Z.-Y. and Sun, Y.-H.* (2013). Subtractive phage display technology identifies zebrafish marcksb that is required for gastrulation. Gene 521, 69-77.
14)Ishtiaq Ahmed, A. S., Xiong, F., Pang, S. C., He, M. D., Waters, M. J., Zhu, Z. Y. and Sun, Y. H.* (2011). Activation of GH signaling and GH-independent stimulation of growth in zebrafish by introduction of a constitutively activated GHR construct. Transgenic Res 20, 557-567.
15)Chen, C.-H., Sun, Y.-H.*, Pei, D.-S. and Zhu, Z.-Y. (2009). Comparative Expression of Zebrafish Lats1 and Lats2 and Their Implication in Gastrulation Movements. Developmental Dynamics 238, 2850-2859.
16)Liu, Y., Bourgeois, C. F., Pang, S., Kudla, M., Dreumont, N., Kister, L., Sun, Y.-H., Stevenin, J. and Elliott, D. J. (2009). The Germ Cell Nuclear Proteins hnRNP G-T and RBMY Activate a Testis-Specific Exon. PLoS Genetics 5 (11): e1000707.
17)Liu, J., Sun, Y. H.*, Wang, Y. W., Wang, N., Pei, D. S., Wang, Y. P., Hu, W. and Zhu, Z. Y. (2008). Identification of differential transcript profiles between mutual crossbred embryos of zebrafish (Danio rerio) and Chinese rare minnow ( Gobiocypris rarus) by cDNA-AFLP. Theriogenology 70, 1525-1535.
18)Pei, D. S., Sun, Y. H.*, Chen, C. H., Chen, S. P., Wang, Y. P., Hu, W. and Zhu, Z. Y. (2008). Identification and characterization of a novel gene differentially expressed in zebrafish cross-subfamily cloned embryos. BMC Dev Biol 8, 29.
19) Pei, D. S., Sun, Y. H.*, Chen, S. P., Wang, Y. P., Hu, W. and Zhu, Z. Y. (2007). Identification of differentially expressed genes from the cross-subfamily cloned embryos derived from zebrafish nuclei and rare minnow enucleated eggs. Theriogenology 68, 1282-1291.
20)Pei, D. S., Sun, Y. H.*, Chen, S. P., Wang, Y. P., Hu, W. and Zhu, Z. Y. (2007). Zebrafish GAPDH can be used as a reference gene for expression analysis in cross-subfamily cloned embryos. Anal Biochem 363, 291-293.
21)Wang, N., Sun, Y. H.*, Liu, J., Wang, Y. P. and Zhu, Z. Y. (2007). Molecular characterization of common carp (Cyprinus carpio) Sonic Hedgehog and discovery of its maternal expression. Development Genes and Evolution 217, 299-305.
22)Wang, N., Sun, Y. H.*, Liu, J., Wu, G., Su, J. G., Wang, Y. P. and Zhu, Z. Y. (2007). Knock down of gfp and no tail expression in zebrafish embryo by in vivo-transcribed short hairpin RNA with T7 plasmid system. Journal of Biomedical Science 14, 767-776.
23)Liu, J., Sun, Y. H.*, Wang, N., Wang, Y. P. and Zhu, Z. Y. (2006). Upstream regulatory region of zebrafish lunatic fringe: Isolation and promoter analysis. Marine Biotechnology 8, 357-365.
24) Sun, Y. H., Chen, S. P., Wang, Y. P., Hu, W. and Zhu, Z. Y. (2005). Cytoplasmic impact on cross-genus cloned fish derived from transgenic common carp (Cyprinus carpio) nuclei and goldfish (Carassius auratus) enucleated eggs. Biol Reprod 72, 510-515.
25)Liu, W. Y., Wang, Y., Sun, Y. H.*, Wang, Y. P., Chen, S. P. and Zhu, Z. Y. (2005). Efficient RNA interference in zebrafish embryos using siRNA synthesized with SP6 RNA polymerase. Development Growth & Differentiation 47, 323-331.
26)Wu, B., Sun, Y. H.*, Wang, Y. W., Wang, Y. P. and Zhu, Z. Y. (2005). Characterization of transgene integration pattern in F4 hGH-transgenic common carp (Cyprinus carpio L.). Cell Research 15, 447-454. (* co-first author)
27) Wu, B., Sun, Y. H.*, Wang, Y. P., Wang, Y. W. and Zhu, Z. Y. (2004). Sequences of transgene insertion sites in transgenic F4 common carp. Transgenic Research 13, 95-96. (* co-first author)
28)Wu, G., Sun, Y. H.* and Zhu, Z. Y. (2003). Growth hormone gene transfer in common carp. Aquatic Living Resources 16, 416-420. (* co-first author)
29) Sun, Y., Chen, S., Wang, Y. and Zhu, Z. (2000). The onset of foreign gene transcription in nuclear-transferred embryos of fish. Sci China C Life Sci 43, 597-605.
30)Zhu, Z. Y. and Sun, Y. H. (2000). Embryonic and genetic manipulation in fish. Cell Research 10, 17-27.
31)張峰華, 王厚鵬, 黃思雨, 熊鳳, 朱作言, 孫永華 * (2016). 兩種密碼子最佳化的 Cas9 編碼基因在斑馬魚胚胎中基因敲除效率的比較. 遺傳 38, 144-154.
發明專利目錄
1) 孫永華，龐少臣，康景軒，朱作言.一種提高魚類omega-3多不飽和脂肪酸的方法及套用.2016年獲中國發明專利授權，專利號：ZL 201410155088.4
2) 孫永華，熊鳳，魏志強，朱作言.一種定向篩選同源重組PGC的載體及構建方法和套用. 2015年獲中國發明專利授權，專利號：ZL 201310341471.4
3) 孫永華,朱作言,熊鳳, Ishtiaq Ahmed, Mike Waters.一種魚類持續激活生長激素受體基因及製備方法和用途. 2013年獲中國發明專利授權，專利號：ZL 201110141084.7.
