人物簡介
二級學科 分析化學畢業院校 南開大學
生物化學分析
(1)DNA-Hemin過氧化物模擬酶套用研究
(2)小分子與DNA的相互作用研究。
承擔科研項目:
1. 螢光偏振基因探針及其在腫瘤診斷中的套用研究,國家自然科學基金(20405007) 23萬元 (已結題)
2.金屬配合物對端粒酶的調控作用及篩選研究,國家自然科學基金(20675041) 28萬元 (2007.1-2009.12)
3. DNA-hemin過氧化物模擬酶體系的構效關係及套用研究,國家自然科學基金(20975055) 34萬元 (2010.1-2012.12)
4. 端粒酶活性的特異性定量檢測技術及診斷試劑盒的開發,天津市套用基礎及前沿技術研究計畫(08JCZDJC21200)20萬元 (2008.4-2010.4)
參與項目
1. 分子探針技術若干關鍵問題的研究(2006CB705700) 973項目
2008年獲天津市自然科學基金二等獎(第二完成人)
發表SCI收錄論文30餘篇,影響因子大於3.0的15篇,其中大於5.0的6篇
近幾年代表性文章:
1.-Ming Kong,* Ya-Wei Jin, Yu-Ji Yin, Huai-Feng Mi, Han-Xi Shen. Real-time PCR detection of telomerase activity using specific molecular beacon probes. Analytical and Bioanalytical Chemistry, 2007, 388, 699-709
2. De-Ming Kong,* Jing Wu, Yong-E Ma, Han-Xi Shen, A new method for the study of G-quadruplex ligands. Analyst 2008, 133, 1158-1160.
3.Ming Kong,* Jiao Wang, Li-Na Zhu, Ya-Wei Jin, Xiao-Zeng Li, Han-Xi Shen, Huai-Feng Mi, Oxidative DNA cleavage by Schiff base tetraazamacrocyclic oxamido nickel(II) complexes. Journal of Inorganic Biochemistry 2008, 102, 824-832.
4. De-Ming Kong,* Yong-E Ma, Jing Wu, Han-Xi Shen,Discrimination of G-quadruplexes with duplex and single-stranded DNAs by fluorescence and energy transfer fluorescence spectra of crystal violet. Chemistry - A European Journal 2009, 15, 901-909.
5. De-Ming Kong*, Yong-E Ma, Jun-Hong Guo, Wei Yang, Han-Xi, Shen, Fluorescent sensor for monitoring structural changesof G-quadruplexes and detection of potassium ion. Analytical Chemistry 2009, 81, 2678-2684.
6. De-Ming Kong,* Li-Li Cai, Jun-Hong Guo, Han-Xi Shen,Characterization of the G-quadruplex structure of a catalytic DNA with peroxidase activity, Biopolymers 2009, 91, 331-339.
7. De-Ming Kong*,Jun-Hong Guo, Jing Wu, Yong-E Ma, Han-Xi Shen, Crystal violet-G-quadruplex complexes as fluorescent sensors for homogeneous detection of potassium ion. Biosensor & Bioelectronics 2009, 25, 88-93.
8. Xue-Hui Zhou, De-Ming Kong*,Han-Xi Shen, Ag+ and Cysteine Quantitation Based on G-Quadruplex-Hemin DNAzymes Disruption by Ag+. Analytical Chemistry, 2010, 1216, 807-814.