![吳曉萍[暨南大學生命科學技術學院 研究員 博導]](/img/8/cea/nBnauM3X1UjNykDOyEzNygzM1UTM1QDN5MjM5ADMwAjMwUzLxczLwYzLt92YucmbvRWdo5Cd0FmL0E2LvoDc0RHa.jpg "吳曉萍[暨南大學生命科學技術學院 研究員 博導]")
人物簡介
吳曉萍： 女 ，暨南大學生命科學技術學院研究員 博導 。近3年主持國家及省部級項目5項。近5年來在國際期刊上發表SCI論文20多篇，其中以第一作者（或通訊作者）在A1類刊物發表5篇，在A2類刊物發表10篇。獲國家科學技術發明二等獎1項、廣東省科學技術發明一等獎1項和中華醫學科技獎一等獎1項。獲發明專利2項，參編著作2部。2010年被評為廣東省高等學校“千百十工程”省級培養對象。
工作經歷
1996.7～1999.10 廣東省昆蟲研究所工作
1999.10～2005.5 暨南大學醫藥生物技術研究開發中心工作
2005.6～至今 暨南大學組織移植與免疫研究中心工作
研究方向
一直致力於細胞因子與創傷修復、腫瘤的基礎理論、臨床套用及其相關基因工程藥物的研發，主要涉及成纖維細胞生長因子（FGF）家族的結構與功能關係、細胞效應與分子調控機制、FGF規模化生產的關鍵技術、臨床套用安全性等方面。目前制約基因工程藥物研發的主要瓶頸問題是基因工程藥物的設計、改造及其高通量篩選，而蛋白質構效關係及其作用機制的研究是基因工程藥物設計和改造的核心基礎。本人十多年來的研究工作一直圍繞細胞因子進行，致力於將成纖維細胞生長因子（FGF）的基礎研究與臨床套用密切結合，在對FGF的結構與功能關係、細胞效應與分子調控機制等基礎問題進行深入研究的基礎上，一方面通過對FGF進行精細的結構改造和修飾，使改構的FGF更適於臨床套用，成功研發了基因工程一類新藥“重組人酸性成纖維細胞生長因子”；另一方面構建了細胞因子拮抗肽的篩選平台，從隨機肽庫中篩選獲得了新型的以bFGF/FGFR為靶標的拮抗肽，並對其作用機制進行了深入研究。
主要論文
1. Cong Wang, Yonglin Yu,Quchou Li, SusuGao, XinlongJia, Xilei Chen, Ruixue Wang, Tao Li, Wenhui Wang, , Xiaokun Li, and Xiaoping Wu*.P7 peptides suppress the proliferation of K562 cells induced by basic fibroblast growth factor.Tumour Biol. 2012. DOI 10.1007/s13277-012-0348-9.
2. Yonglin Yu, SusuGao, Quchou Li, Cong Wang, XinqiangLai, Xilei Chen, Ruixue Wang, JingfangDi , Tao Li, Wenhui Wang, Xiaoping Wu*. The FGF2-binding peptide P7 inhibits melanoma growth in vitro and in vivo. J Cancer Res Clin Oncol. 2012. DOI 10.1007/s00432-012-1201-7
3. Quchou Li, SusuGao, Yonglin Yu, Wenhui Wang, Xilei Chen, Ruixue Wang, Tao Li, Cong Wang, Xiaokun Li,Xiaoping Wu*. A novel bFGF antagonist peptide inhibits breast cancer cell growth. Mol Med Report. 2012, 6(1):210-214.
4. Xiaoping Wu*, XinglongJia, YuhuaJi, Cong Wang,, Yonglin Yu, SusuGao, Quchou Li, Xiaokun Li. Effects of a Synthetic bFGF Antagonist Peptide on the Proteome of 3T3 Cells Stimulated with bFGF. Int J Pept Res Ther. 2011, 17(1): 53–59.
5. HaishanTian, Lu Tang, Yi Wang, Xiaojie Wang, Lili Guan, Jian Zhang. Xiaoping Wu*, Xiaokun Li. Lactose Induction Increases Production of RecombinantKeratinocyte Growth Factor-2 in Escherichia coli. Int J Pept Res Ther. 2011, 17(2): 123–129.
6. Zhifeng Huang, Huiyan Wang, Meifei Lu, Chuanchuan Sun, Xiaoping Wu, Yi Tan, Chaohui Ye, Guanghui Zhu, Xiaojie Wang, Lu Cai, Xiaokun Li A Better Anti-Diabetic Recombinant Human Fibroblast Growth Factor 21 (rhFGF21) Modified with Polyethylene Glycol. PLoS One. 2011,6(6):e20669.
7. Yi Wanghttp://pubs.acs.org/doi/abs/10.1021/jm200017g - notes-1#notes-1, Jian Xiaohttp://pubs.acs.org/doi/abs/10.1021/jm200017g - notes-1#notes-1, Huiping Zhou, Shulin Yang, Xiaoping Wu, Chengxi Jiang, Yunjie Zhao, Donglou Liang, Xiaokun Li, and Guang Liang. A Novel Monocarbonyl Analogue of Curcumin, (1E,4E)-1,5-Bis(2,3-dimethoxyphenyl)penta-1,4-dien-3-one, Induced Cancer Cell H460 Apoptosis via Activation of Endoplasmic Reticulum Stress Signaling Pathway. J Med Chem. 2011,54(11):3768-78.
8. Xiaoping Wu, Qiuxia Yan, Yadong Huang, Huixian Huang, Zhijian Su, Jian Xiao, Yaoying Zeng, Yi Wang, Changjun Nie, Yongguang Yang, Xiaokun Li.Isolation of a novel basic FGF-binding peptide with potent antiangiogenetic activity.J Cell Mol Med. 2010, 14(1-2): 351-356.
9. Cong Wang, Shaoqiang Lin, YanfangNie, XinglongJia, Jing Wang, Jian Xiao, Jianzhang Wu, Xiaokun Li, Xiaoping Wu*. Mechanism of antitumor effect of a novel bFGF binding peptide on human colon cancer cells.Cancer Science. 2010. 101(5): 1212-1218.
10. Chengguang Zhao, Ju Yang, Yi Wang, Donglou Liang, Xuyi Yang, Xiaoxia Li, Jianzhang Wu, Xiaoping Wu, Shulin Yang, Xiaokun Li, Guang Liang. Synthesis of mono-carbonyl analogues of curcumin and their effects on inhibition of cytokine release in LPS-stimulated RAW 264.7 macrophages. Bioorg Med Chem. 2010, 18(7):2388-93.
11. Xiaoping Wu, Haishan Tian, Yadong Huang, Sixian Wu, Xiaoju Liu, Cong Wang, Xiaojie Wang, Zhifeng Huang, Jian Xiao, Wenke Feng, Xiaokun Li. Large-scale production of biologically active human keratinocyte growth factor-2.Appl Microbiol Biotechnol. 2009, 82(3): 439-444.
12. Xiaoping Wu, Changjun Nie, ZhifengHuang, YanfangNie, Qiuxia Yan, Yecheng Xiao, Zhijian Su, Yadong Huang, Jian Xiao, Yaoying Zeng, Yi Tan, Wenke Feng, Xiaokun Li. Expression and Purification of Human Keratinocyte Growth Factor 2 by Fusion with SUMO.Mol Biotechnol. 2009, 42(1): 68-74.
13. He F, Zeng Y, Wu X, Ji Y, He X, Andrus T, Zhu T, Wang T. Endogenous HIV-1 Vpr-mediated apoptosis and proteome alteration of human T-cell leukemia virus-1 transformed C8166 cells. Apoptosis. 2009, 14(10):1212-26.
14. Zhifeng Huang, Chunyan Ni, Yanhui Chu, Shanshan Wang, Shulin Yang,Xiaoping Wu, Xiaojie Wang, Xiaokun Li, WenkeFeng, Shaoqiang Lin. Chemical modification of recombinant human keratinocyte growth factor 2 with polyethylene glycol improves biostability and reduces animal immunogenicity. J Biotechnol. 2009, 142(3-4):242-9.
15.Yadong Huang, YulanRao, ChengliFeng, Yanmei Li, Xiaoping Wu, Zhijian Su, Jian Xiao, Yechen Xiao, WenkeFeng, Xiaokun Li. High-level expression and purification of Tat-haFGF19-154. ApplMicrobiolBiotechnol, 2008, 77(5): 1015-1022.
16. Xiaoping Wu, Xiaoju Liu, Yecheng Xiao, Zhifeng Huang, Jian Xiao, Shaoqiang Lin, Lu Cai, WenkeFeng, Xiaokun Li. Purification and modification by polyethylene glycol of a new human basic fibroblast growth factor mutant-hbFGFSer25,87,92. Journal of Chromatography A, 2007,1161(1-2): 51-55.
17. Xiaokun Li, LijuanJiang, Yi Wang, YechengXiao, YadongHuang, QiongfengYao, YongguangYang, Xiaoping Wu*. Inhibition of angiogenesis by a novel small peptide consisting of the active fragments of platelet factor-4 and vasostatin.Cancer Lett. 2007, 256(1): 29-32.
18. Su Z, Wu X, Feng Y, Ding C, Xiao Y, Cai L, Feng W, Li X. High level expression of human endostatin in Pichiapastoris using a synthetic gene construct.Appl Microbiol Biotechnol. 2007, 73(6):1355-62.
19. Huang Z, Zheng Q, Wu X, Su Z, Xu H, Tan Y, Feng W, Li X, Cai L.Enhanced protection of modified human acidic fibroblast growth factor with polyethylene glycol against ischemia/reperfusion-induced retinal damage in rats.Toxicol Lett. 2007, 170(2):146-56.
20.Xiaoping Wu,Xiaokun Li,YaoyingZeng,Qing Zheng,Sixian Wu. Site-directed PEGylation of human basic fibroblast growth factor. Protein Expression and Purification,2006, 48(1): 24-27.
21.ZhijianSu, YadongHuang, QuannanZhou, ZhilingWu, Xiaoping Wu, Qing Zheng, ChangcaiDing, XiaokunLi.High-level expression and purification of human epidermal growth factor with SUMO fusion in Escherichia coli.Protein Pept Lett. 2006;13(8):785-92.
22.Xiaoping Wu, Zhijian Su, Xiaokun Li, Qing Zheng, Yadong Huang, Hui Yuan. High-level expression and purification of a nonmitogenic form of human acidic fibroblast growth factor in Escherichia coli. Protein Expression and Purification,2005,42(1): 7-11.
23. Xiaoping Wu, Xiaokun Li, Zhijian Su, Qing Zheng, HuaXu, Sixian Wu, YaFeng, Wen Zhao. Expression and purification of soluble non-fusion vasostatin in Escherichia coli.Protein and Peptide Letters, 2005, 12(7): 659-661.
承擔課題：
1. 國家“863”計畫 “新型非促分裂重組成纖維細胞生長因子改構體的研究”子課題, 2005.1-2007.12, 項目負責人。
2. 國家“863”計畫“提高角化生長因子（KGF-2）表達量及穩定性的關鍵技術研究”子課題, 2008.1-2010.12,項目負責人。
3. 國家自然科學基金面上項目, “P7逆轉結腸癌細胞化療耐藥的作用機制研究”, 2011.1-2013.12, 項目負責人。
4. 國家自然科學基金面上項目, “新型bFGF結合肽拮抗bFGF的作用機制研究”, 2010.1-2012.12, 項目負責人。
5. 廣東省自然科學基金面上項目, “bFGF活性拮抗短肽的抗腫瘤作用機制研究”, 2010.1-2012.12，項目負責人。
6. 廣東省科技計畫項目, “晶狀體蛋白在人角質形成細胞中的凋亡調控作用研究”, 2011.1-2013.12,
榮譽與獎勵：
1. 國家科學技術發明二等獎：一類新藥重組成纖維細胞生長因子關鍵工程技術及套用.2009.
2. 廣東省科學技術發明一等獎: 一類新藥重組人酸性成纖維細胞生長因子的開發與套用.2007
3. 中華醫學科技獎一等獎：一類新藥重組成纖維細胞生長因子套用基礎與工程技術研究.2008
4. 中國藥學科學技術一等獎：基因工程一類新藥重組成纖維細胞生長因子（rFGF）系列產品的開發與套用. 2007
