薛勇彪

薛勇彪 簡介:
研究員,遺傳與發育生物學研究所所長。
1983年,蘭州大學生物學系動物學專業理學學士;1986年,中國科學院發育生物學研究所理學碩士;1989年,John Innes Institute 和University of East Anglia博士;1990-1995年,John Innes Centre和University of Oxford博士後。1995-1997年,John Innes Centre 的Sainsbury Laboratory 任Research Scientist。薛勇彪博士系1998年中科院"百人計畫"入選者和國家傑出青年科學基金獲得者。現任研究所所長、國家973計畫"水稻功能基因組學研究"項目首席科學家和《遺傳學報》和《遺傳》主編,山東農業大學, 北京師範大學,蘭州大學和北京大學兼職或客座教授。
研究概述 :
薛勇彪老師實驗室主要研究顯花植物花粉識別的分子機制。目前實驗室的主要研究方向包括:
1. 金魚草自交不親和性的分子機制 :
自交不親和性是研究植物花粉識別的模式系統。在以茄科植物為代表的配子體自交不親和植物中,目前的研究結果表明這些植物的自交不親和性位點-S-位點編碼兩類不同的基因分別控制花柱和花粉自交不親和性的表達。花柱基因編碼了一類核酸酶稱為S-核酸酶,而花粉基因則編碼一類新的F-box基因 (S-locus F-box, SLF),共同構成一個S-單倍型(S-Haplotype)控制自交不親和性的表達。我們實驗室以金魚草為模式系統克隆了SLF家族的第一個成員-AhSLF-S2,並證明它控制了花粉自交不親和性的表達,並且它編碼的蛋白質通過形成一個SCF(Skp1/Cullin or CDC53/F-box)複合體與S- 核酸酶發生作用。與此同時,我們進一步證明泛素/26S蛋白小體介到的蛋白質降解途徑特異性的參與了金魚草的親和反應。但是,這兩類蛋白質如何相互作用產生特異性花粉識別的機理還不清楚。我們實驗室目前的主要工作是利用分子遺傳,生物化學和細胞生物學等手段,研究這些蛋白質的作用機理以及S-位點的起源和進化機制。
2.水稻傳粉和受精的分子控制 :
傳粉和受精是種子形成的重要基礎。但是我們對於它的分子機理了解還非常不夠。目前我們構建了一個由1萬多條非冗餘cDNA組成的微陣列,並且獲得了水稻傳粉和受精相關的基因表達譜,克隆了一批候選基因,正在詳細鑑定它們的生物學功能。同時,我們與福建農業大學的吳為人和段遠霖合作圖位克隆控制水稻開花、花器官發育的關鍵基因。
3. 棉花纖維發育相關基因的克隆和功能鑑定 :
棉花纖維是胚珠表皮細胞特化的結果。通常30%左右的表皮細胞分化形成棉纖維。目前關於胚珠表皮細胞分化成纖維的分子機理的了解非常有限。利用同源基因克隆的途徑,我們分離鑑定了50多個在棉纖維發育早期表達的MYB類轉錄因子,並發現了一個在纖維發育起始期和伸長期特異表達基因GhMYB109。目前我們正在利用轉基因等方法研究該基因在棉纖維發育中的作用機理。
近期發表研究論文:
1. Li, H., Xue, D., Gao, Z., Yan, M., Xu, W., Xing, Z., Huang, D., Qian, Q., Xue, Y. (2009) A putative lipase gene EXTRA GLUME1 regulates both empty glume fate and spikelet development in rice. Plant J. 57:593-605
2. Zhang, Y., Xu, W., Li, Z., Deng, X., Wu, W., Xue, Y. (2008) F-box protein DOR functions as a novel inhibitory factor for ABA-induced stomatal closure under drought stress in Arabidopsis thaliana. Plant Physiol. 148:2121-2133
3. Xu, S., Deng, Z., Chong, K., Xue, Y., Wang, T. (2008) Dynamic proteomic analysis reveals a switch between central carbon metabolism and alcoholic fermentation in Oryza sativa filling grains. Plant Physiol. 148:908-925
4. Pu, L., Li, Q., Fan, X., Yang, W., Xue, Y. (2008) A R2R3 MYB transcription factor GhMYB109 is required for cotton fiber development. Genetics 180:811-820
5. Chen, J., Ding, J., Ouyang, Y., Du, H., Yang, J., Cheng, K., Zhao, J., Qiu, S., Zhang, X., Yao, J., Liu, K., Wang, L., Xu, C., Li, X., Xue, Y., Xia, M., Ji, Q., Lu, J., Xu. M., Zhang, Q. (2008) A tri-allelic system of S5 is a major regulator of the reproductive barrier and compatibility of indica-japonica hybrids in rice. Proc. Natl. Acad. Sci. USA 105:11436-11441
6. Yang, W., Kong, Z., Omo-Ikerodah, E., Xu, W., Li, Q., Xue, Y. (2008) A calcineurin B-like interacting protein kinase OsCIPK23 functions in pollination and drought stress responses in rice (Oryza sativa L.). JGG 35:531-543
7. Yang, W., Lai, Y., Li, M., Xu, W., Xue, Y. (2008) A novel C2-domain phospholipid binding protein, OsPBP1, is required for pollen fertility in rice. Mol Plant 1:770-785
8. Zhang, D., Yang, Q., Ding, Y., Cao, X., Xue, Y., Cheng, Z. (2008) Cytological characterization of the tandem repetitive sequences and their methylation status in antirrhinum majus genome. Genomics 92:107-114
9. Liu, F., Xu, W., Tan, L., Xue, Y., Sun, C., Su, Z. (2008) Case study for identification of InDel-caused alternative expression isoforms in rice subspecies japonica and indica by integrative genome analysis. Genomics 91:186-194
10. Li, M., Xu, W., Yang, W., Kong, Z., Xue, Y. (2007) Genome-wide gene expression profiling reveals conserved and novel molecular functions of the stigam in rice (Oryza sativa L.). Plant Physiol. 144:1797-1812
11. Yang, Q., Zhang, D., Li, Q., Cheng, Z., Xue, Y. (2007) Heterochromatic and genetic features are consistent with recombination suppression of the self-incompatibility (S)-locus in Antirrhinum. Plant J. 51:140-151
12. Wang, X, Zhang, Y., Ma, Q., Zhang, Z., Xu, Z., Xue, Y., Bao, S., Chong, K. (2007) SKB1-mediated symmetric dimethylation of histone H4R3 controls flowering time in Arabidopsis. EMBO J. 26:1934-1941
13. Dai, S., Chen, T., Chong, K., Xue, Y., Li, S., Wang, T. (2007) Proteomic identification of differentially expressed proteins associated with pollen germination and tube growth reveals characteristics of germinated Oryza sativa pollen. Mol. Cell. Proteomics 6:207-230
14. Kong, Z., Li, M., Yang, W., Xu, W., Xue, Y. (2006) A novel nuclear-localized CCCH-type zinc finger protein, OsDOS, is involved in delaying leaf senescence in rice (Oryza sativa L.). Plant Physiol. 141:1376-1388
15. Huang, J., Zhao, L., Yang, Q., Xue, Y. (2006) AhSSK1, a novel SKP1-like protein that interacts with the S-Locus F-box protein.Plant J. 46:780-793
16. Dai, S., Li, L., Chen, T., Chong, K., Xue, Y., Wang, T. (2006) Proteomic analyses of Oryza sativa mature pollen reveal novel proteins associated potentially with pollen germination and tube growth. Proteomics 6:2504-2529
17. Dong, L., Wang, L., Zhang, Y., Zhang, Y., Deng, X., Xue, Y. (2006) An auxin-inducible F-box protein CEGENDUO negatively regulates auxin-mediated lateral root formation in Arabidopsis. Plant Mol Biol. 60:599-616

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