個人經歷
教育經歷
① 1991.9-1995.7:河南科技學院園藝系園藝專業學習,獲學士學位。
② 1997.9-2000.12:西北農林科技大學園藝學院攻讀碩士學位,獲碩士學位。
③ 2001.9-2006.4:西北農林科技大學園藝學院攻讀博士學位,獲博士學位。
工作經歷
① 2011.03-2011.08: 維也納自然資源與套用生命科學大學套用植物科學和生物技術系Astrid Forneck教授實驗室做博士後研究。
② 2008.12-至今: 西北農林科技大學園藝學院副教授,碩士生導師。
③ 2007.05-2008.10: 北京生命科學研究所鄧興旺教授實驗室做博士後研究。
④ 2006.04-2007.4: 耶魯大學分子、細胞和發育生物學系鄧興旺教授實驗室做博士後研究。
⑤ 2001.02-2005.12: 西北農林科技大學園藝學院講師。
研究興趣
本實驗室的研究興趣主要是中國野生葡萄如何感受並回響葡萄白粉病病原真菌脅迫,特別是中國野生葡萄在白粉病病原真菌脅迫條件下體內抗病信號轉導和抗病基因的調節。主要研究內容是克隆和分析中國野生葡萄重要抗病基因和關鍵作用元件功能;利用生物化學試驗體外分析目的基因的功能,並利用模式植物擬南芥突變體研究植物感受病原真菌脅迫信號過程中所發生的遺傳和分子特性的變化。目的是為了闡明中國野生葡萄回響白粉病病原真菌脅迫的信號途徑,並鑑定一些具有潛在功能的能夠提高作物適應病原真菌脅迫的關鍵作用元件。
開設課程
承擔本科生《植物育種學》(雙語課程)、《試驗設計與分析》、《果樹育種學》、《果樹科研專題》、《園藝學概論》等課程的教學工作;合作承擔碩士、博士研究生《現代生物技術》、《園藝學專題講座》、《現代園藝植物科學研究進展》等課程,現指導碩士研究生6名。
學術成果
1997年以來,師從王躍進教授一直從事葡萄種質資源評價與遺傳育種研究工作。參加並完成公益性行業(農業)農業科研專項、國家自然科學基金、國家“863”計畫子項目、國家轉基因植物研究與產業化開發專項、農業部“948”項目、科技部“十一五”科技支撐計畫等項目多項。獲陝西省科學技術壹等獎1項(第五完成人);參編專著2部 。
(1)主持科研項目情況
(1)國家自然科學基金面上基金項目“葡萄霜黴菌效應因子與中國野生葡萄蛋白互作生物學功能研究(31672115)”,2017.01-2020.12.
(2)國家自然科學基金面上基金項目“葡萄霜黴菌效應因子篩選及中國野生葡萄抗霜霉病基因鑑定(31471844)”,2015.01-2018.12.
(3)國家自然科學基金面上基金項目“白粉病誘導的中國野生華東葡萄VpPR10蛋白基因定位表達研究(31272125)”,2013.01-2016.12.
(4)國家自然科學基金青年基金項目“中國野生葡萄PR10蛋白功能及抗病原真菌活性分析(30700547)”,2008.01-2010.12.
(5)國家自然科學基金專項基金項目“中國野生葡萄紫檀芪生物合成機理解析(30940051)”,2010.01-2010.12.
(6)教育部“新世紀優秀人才支持計畫”項目“中國野生葡萄VpPR10蛋白調節植物細胞死亡機理研究(NCET-10-0692)”,2011.01-2013.12.
(7)國家林業局“948”項目“葡萄砧木新品種和優質苗木繁育技術引進(2013-4-41)”,2013.01-2015.12.
(8)農業部“公益性行業(農業)”科研專項子課題“主要果樹砧木收集、評價與篩選(201203075-08)”,2012.01-2016.12.
(9)農業部“948”(重大專項滾動)項目子課題“重要果樹基因資源發掘與創新的關鍵技術合作研發(2011-G21)”,2011.01-2016.12.
(10)農業部“2012年物種資源保護”項目“陝西省農業野生植物資源調查”,2012.01-2012.12.
(11)陝西省科技統籌創新工程計畫項目“葡萄優異種質資源創新與高效栽培技術研究(2013KTCL02-01)”,2014.01-2016.12.
(12)西北農林科技大學“優秀青年人才科研專項”項目“華東葡萄VpPR10.1抗病機理研究(QN2011052)”,2011.09-2014.09.
(13)西北農林科技大學“青年學術骨幹支持計畫”項目“中國野生葡萄白藜蘆醇-氧-甲基轉移酶基因克隆及原核表達研究”,2010.01-2012.12.
(14)西北農林科技大學試驗示範站(基地)科技創新與成果轉化項目“葡萄砧木新品種選育與中試示範(NYY2013-12)”,2013.01-2014.12.
主要論文
發表的主要研究論文(#:通訊作者或第一作者)
(1)Lijuan Han, Kai Weng, Hui Ma, Gaoqing Xiang, Zhiqian Li, Yuejin Wang, Guotian Liu and Yan Xu#. Identification and Characterization of Erysiphe necator-Responsive MicroRNAs in Chinese Wild Vitis pseudoreticulata by High-Throughput Sequencing. Frontiers in Plant Science, 2016, 7:621.
(2)Zhenhua Cui, Wenlu Bi, Xinyi Hao, Yan Xu#, Pengmin Li, Andrew Walker, Qiaochun Wang. Responses of in vitro-grown plantlets (Vitis vinifera) to Grapevine leafroll-associated virus-3 and PEG-induced drought stress. Frontiers in Physiology, 2016, 7:203.
(3)Lingmin Dai, Dan Wang, Xiaoqing Xie, Chaohong Zhang, Xiping Wang, Yan Xu, Yuejin Wang, Jianxia Zhang. The Novel Gene VpPR4-1 from Vitis pseudoreticulata Increases Powdery Mildew Resistance in Transgenic Vitis vinifera L. Frontiers in Plant Science, 2016, 7:695.
(4)Bihai Shi, Cui Zhang, Caihuan Tian, Jin Wang, Quan Wang, Tengfei Xu, Yan Xu, Carolyn Ohno, Robert Sablowski, Marcus G. Heisler, Klaus Theres, Ying Wang*, and Yuling Jiao*. Two-step Regulation of a Meristematic Cell Population Acting in Shoot Branching in Arabidopsis. PLoS Genetics, 2016, Jul 11;12(7):e1006168.
(5)Zhiqian Li, Tiemei Li, Yuejin Wang, Yan Xu#. Breeding new seedless grapes using in ovulo embryo rescue and marker-assisted selection. In Vitro Cellular and Developmental Biology, 2015, 51:241-248.
(6)Ruiqi Liu, Lan Wang, Jiali Zhu, Tingting Liu, Yuejin Wang, Yan Xu#. Histological responses to downy mildew in resistant and susceptible grapevines. Protoplasma. 2015, 252(1): 259-270.
(7)Z.-H. Cui, W.-L. Bi, C. Pan, Y. Xu and Q.-C. Wang. Abiotic stress improves in vitro biological indexing of Grapevine leaf roll-associated virus-3 in red grapevine cultivars. Australian Journal of Grape and Wine Research, 2015, 21:490-495.
(8)Yan Xu#, Yuejin Wang. Introduction of Application of Chinese Wild Grapes Species. 10th International Conference on Grapevine Breeding and Genetics, 2010/8/1-2010/8/5, pp 49-56, Geneva, NY, 2014.
(9)Lan Wang, Jinyu Wei, Ying Zou, Keyao Xu, Yuejin Wang, Lu Cui,Yan Xu#. Molecular Characteristics and Biochemical Functions of VpPR10s from Vitis pseudoreticulata Associated with Biotic and Abiotic Stresses. International Journal of Molecular Sciences, 2014, 15, 19162-19182.
(10)Kai Weng, Zhi-Qian Li, Rui-Qi Liu, Lan Wang, Yuejin Wang, Yan Xu#. RNA-Seq Analysis of the Transcriptome of Vitis Pseudoreticulata in Response to Powdery Mildew Infection. Horticulture Research,2014(1), 14049.
(11)Teng-Fei Xu, Xiao-Chen Zhao, Yun-Tong Jiao, Jin-Yu Wei, Lan Wang, Yan Xu#. A pathogenesis related protein, VpPR-10.1, from Vitis pseudoreticulata: An insight of its mode of antifungal activity. PLoS ONE. 2014, 9(4):e95102.
(12)Yihe Yu,Weirong Xu, JieWang, Lei Wang, Wenkong Yao, Yazhou Yang, Yan Xu, Fuli Ma, Yangjian Du and Yuejin Wang. The Chinese wild grapevine (Vitis pseudoreticulata) E3 ubiquitin ligase Erysiphe necator-induced RING ?nger protein 1 (EIRP1) activates plant defense responses by inducing proteolysis of the VpWRKY11 transcription factor. New Phytologist. 2013, 200(3):834-846.
(13)XIN GUAN, HEQING ZHAO, YAN XU, YUEJIN WANG. Studies on gene transfer of shoot apical meristems by Agrobacterium-mediated genetic transformation in a progeny of Chinese wild Vitis pseudoreticulata. Vitis 2013, 52 (4):185-192.
(14)Teng-Fei Xu, Jiang-Xiang, Feng-Ju Li, Tie-Mei Li, Yi-He Yu, Yue-Jin Wang, Yan Xu#. Screening proteins interacting with VpPR10.1 of Chinese wild grapevine using the yeast-two hybrid system. Acta Physiologiae Plantarum. 2013, 35(8): 2355-2364.
(15)J. Xiang, R. Q. Liu, T. M. Li, L. J. Han, Y. Zou, T. F. Xu, J. Y. Wei, Y. J. Wang, Y. Xu#. Isolation and Characterization of Two VpYABBY Genes from Chinese Wild Vitis pseudoreticulata. Protoplasma. 2013, 250:1315-1325.
(16)Mingyang He, Yan Xu#, Jiangling Cao, Ziguo Zhu, Yuntong Jiao, Yuejin Wang, Xin Guan, Yazhou Yang, Weirong Xu, Zhenfang Fu. Subcellular localization and functional analyses of a PR10 protein gene from Vitis pseudoreticulata in response to Plasmopara viticola infection. Protoplasma. 2013, 250:129-140.
(17)Ziguo Zhu, Jiangli Shi, Weirong Xu, Huie Li, Mingyang He, Yan Xu, Tengfei Xu, Yazhou Yang, Jiangling Cao, Yuejin Wang. Three ERF transcription factors from Chinese wild grapevine Vitis pseudoreticulata participate in different biotic and abiotic stress-responsive pathways. Journal of Plant Physiology. 2013, 170:923-933.
(18)Yihe Yu, Weirong Xu, Jie Wang, Lei Wang, Wenkong Yao, Yan Xu, Jiahua Ding, Yuejin Wang. A core functional region of the RFP1 promoter from Chinese wild grapevine is activated by powdery mildew pathogen and heat stress. Planta. 2013, 237:293-303.
(19)Heqing Zhao, Xin Guan, Yan Xu#, Yuejin Wang. Characterization of novel gene expression related to Glyoxal oxidase by agro-infiltration of the leaves of accession Baihe-35-1 of Vitis pseudoreticulata. Protoplasma.2013, 250:765-777.
(20)Yazhou Yang, Mingyang He, Ziguo Zhu, Shuxiu Li, Yan Xu, Chaohong Zhang, Stacy D Singer, Yuejin Wang. Identification of the dehydrin gene family from grapevine species and analysis of their responsiveness to various forms of abiotic and biotic stress. BMC Plant Biology 2012, 12:140 DOI: 10.1186/1471-2229-12-140.
(21)Q. Zhou, H. Gao, M.Wang, Y. Xu, Y. Z. Guo, Y. Z. Wan, Z. Y. Zhao. Characterization of defense-related genes in the‘Qinguan’apple in response to Marssonina coronaria. South African Journal of Botany. 2012, DOI:10.1016/j.sajb.2012.01.005.
(22)Yan Xu#, Tengfei Xu, Xiaochen Zhao, Ying Zou, Zhiqian Li, Fengju Li, Jiang Xiang, Yuejin Wang. Co-expression of VpROMT gene from Chinese wild Vitis pseudoreticulata with VpSTS in tobacco plants and its effects on the accumulation of pterostilbene. Protoplasma. 2012, 249: 819-833.
(23)Yihe Yu, Weirong Xu, Shengyi Wang, Yan Xu , Hui'e Li, Yuejin Wang , Shuxiu Li. VpRFP1, a novel C4C4-type RING finger protein gene from Chinese wild Vitis pseudoreticulata, functions as a transcriptional activator in defence response of grapevine. Journal of Experimental Botany. 2011, 62(15): 5671-5682.
(24)Xin Guan, Heqing Zhao, Yan Xu, Yuejin Wang. Transient expression of glyoxal oxidase from the Chinese wild grape Vitis pseudoreticulata can suppress powdery mildew in a susceptible genotype. Protoplasma. 2011, 248: 415-423.
(25)Weirong Xu, Yihe Yu, Qi Zhou, Jiahua Ding, Lingmin Dai, Xiaoqing Xie, Yan Xu, Chaohong Zhang, Yuejin Wang. Expression pattern, genomic structure and promoter analysis of the gene encoding stilbene synthase from Chinese wild Vitis pseudoreticulata. Journal of Experimental Botany. 2011, 62(8):2745-2761.
(26)Yan Xu#, Hao Yu, Mingyang He, Yazhou Yang, Yuejin Wang. Isolation and expression analysis of a novel pathogenesis-related protein 10 gene from Chinese wild Vitis pseudoreticulata induced by Uncinula necator. Biologia. 2010, 65 (4): 653-659.
(27)Hui’e Li,Yan Xu,Yu Xiao,Ziguo Zhu,Xiaoqing Xie,Heqing Zhao,Yuejin Wang. Expression and functional analysis of two genes encoding transcription factors, VpWRKY1 and VpWRKY2, isolated from Chinese wild Vitis pseudoreticulata. Planta. 2010, 232: 1325-1337.
(28)Yan Xu#, Yue-jin Wang. Construction of a cDNA Library of Vitis pseudoreticulata native to China inoculated with Uncinula necator and the analysis of potential defence-related expressed sequence tags (ESTs). South African Journal of Enology and Viticulture. 2009, 30(1):65-71.
(29)Yan Xu#, Yue-jin Wang. Molecular cloning and characterization of novel heat shock protein 90 gene from a wild Vitis pseduoreticulata native to China. Biologia . 2009, 64 (1): 102-106.