![楊淑華[中國農業大學生物學院教授]](/img/c/b9f/nBnauM3XzQjM5ADN5EjN5ATO1UTM1QDN5MjM5ADMwAjMwUzLxYzL2QzLt92YucmbvRWdo5Cd0FmLxE2LvoDc0RHa.jpg "楊淑華[中國農業大學生物學院教授]")
學習經歷
1987年9月－1991年7月，南開大學，理學學士學位
1991年9月－1994年7月，南開大學，理學碩士學位
1998年7月－2002年1月，新加坡國立大學，博士學位
工作經歷
1994年7月－1998年6月，南開大學，助理研究員
2002年2月－2004年12月，美國康奈爾大學，博士後
2005年1月－今，中國農業大學生物學院，教授
研究方向
研究方向：植物回響低溫脅迫的分子機制
作為外界主要環境因子，溫度影響植物的生長發育、植被的分布以及農作物的產量。我們實驗室主要是通過分子生物學、遺傳學和生物化學等手段，研究植物應答低溫脅迫的信號轉導途徑、低溫信號與植物激素信號之間的互動作用，以及低溫信號與生物脅迫信號之間的互動作用等。
1. 植物回響零下低溫脅迫的分子調控研究
在植物抗凍性研究方面，我們建立了穩定的凍相關突變體的篩選體系。通過該體系，我們發現蛋白激酶CRPK1（Cold-responsive protein kinase 1）參與負向調控低溫信號途徑關鍵轉錄因子CBF依賴的信號轉導。低溫激活定位於細胞膜上的CRPK1，後者磷酸化細胞質中的14-3-3蛋白，誘導其進入細胞核中，與CBF1/3蛋白互作，介導CBF1/3蛋白的泛素化降解，從而負調控植物對低溫的應答。該研究不僅揭示了低溫信號從細胞膜傳遞到細胞核中的負調控機制，也闡明了CBF蛋白翻譯後修飾的調控機制（Liu et al., 2017,Mol Cell）。而蛋白激酶OST1在植物低溫應答中發揮重要的正向調控作用。OST1激酶活性被低溫迅速誘導，激活狀態的OST1將ICE1磷酸化，抑制ICE1的泛素化降解，從而正調控植物的抗凍性（Ding et al., 2015,Dev Cell）。
在低溫信號和植物激素信號的交叉互作方面，我們發現乙烯信號通路關鍵組分均參與低溫應答。轉錄因子EIN3蛋白直接負調控低溫調節基因CBF和細胞分裂素信號負調控因子A型ARR基因的表達，從而負調控擬南芥的抗凍性。該研究明確了乙烯在植物抗低溫中的作用，同時也揭示乙烯和細胞分裂素信號之間互動作用的分子機制（Shi et al., 2012,Plant Cell）。另一個重要植物激素油菜素內酯信號通路關鍵的蛋白激酶BIN2和轉錄因子BZR1/BES1通過依賴和不依賴於CBF的方式調控下游一系列基因的表達，從而正向調節植物的抗凍能力（Li et al., 2017,Mol Plant）。利用CRISPR技術，構建了cbfs三缺失突變體，明確了CBFs在低溫馴化中的關鍵作用及CBFs的靶基因，並為低溫信號研究提供了理想的遺傳材料 (Jia et al., 2016,New Phytol)。
2. 植物回響零上低溫脅迫的分子調控研究
我們的研究發現擬南芥防衛反應中起重要作用的抗病相關基因CHS1、RPP4/CHS2、CHS3和LSD1/CHS4參與不同溫度下植物生長的穩態平衡調控 (Wang et al., 2013,Plant J;Wang et al., 2011,Plant J;Huang et al., 2010,Plant Physiol; Yang et al., 2010,Plant J; Huang et al., 2010,New Phytol)。研究揭示TIR-NB類蛋白CHS1通過與R蛋白SOC3互作，蛋白磷酸酶IBR5和伴侶蛋白HSP90分別與R蛋白CHS3和CHS2互作，調控R蛋白的活性，從而調控R介導的溫度依賴的細胞死亡和防衛反應 (Zhang et al., 2016,New Phytol; Liu et al., 2015,PLoS Genet; Bao et al., 2014,New Phytol)。
研究發現擬南芥磷脂結合蛋白BON1在調控溫度依賴的生長發育中是一個關鍵調控因子，BON1與兩個類受體蛋白激酶BIR1和BAK1互作，可能通過BAK1對BIR1和BON1的磷酸化，作用於同一條信號通路，協同調控溫度依賴的植物生長發育和防衛反應 (Wang et al., 2011,Plant J; Yang et al., 2007,Plant Physiol; Yang et al., 2006,Plant J; Yang and Hua, 2004,Plant Cell)。
發表論文
1. Liu Z, Jia Y, Ding Y, Shi Y, Zhang X, Li Z, Guo Y, Gong Z,Yang S*(2017) Plasma membrane CRPK1-mediated phosphorylation of 14-3-3 proteins induces their nuclear import to fine-tune CBF signaling during cold response.Mol Cell66: 117-128. (Previewed in the same issue of Mol Cell)
2. Li H#, Ye K#, Shi Y, Zhang X,Yang S*(2017) BZR1 positively regulates freezing tolerance via CBF-dependent and CBF-independent pathways in Arabidopsis.Mol Plant 10: 545-559. (#These authors contributed equally) (Spotlighted in the same issue of Mol Plant)
3. Zhang Z#, Li J#, Pan Y#, Li J#, Zhou L#, Shi H#, Zeng Y#, Guo H, Yang S, Zheng W, Yu J, Sun X, Li G, Ding Y, Ma L, Shen S, Dai Ln, Zhang H,Yang S, Guo Y, Li Z* (2017) Natural variation in CTB4a enhances rice adaptation to cold habitats.Nat Commun8:14788. Doi: 10.1038/ncomms14788.
4. Zhang Y#, Wang Y#, Liu J, Ding Y, Wang S, Zhang X, Liu Y,Yang S*(2017) Temperature-dependent autoimmunity mediated by chs1 requires its neighboring TNL gene SOC3.New Phytol213:1330-1345.
5. Jia Y, Ding Y, Shi Y, Zhang X, Gong Z,Yang S*(2016) The cbfs triple mutants reveal the essential functions of CBFs in cold acclimation and allow the definition of CBF regulons in Arabidopsis.New Phytol212: 345-353. (Commentary in the same issue of New Phytol)
6. Huang X, Zhang X, Gong Z,Yang S, Shi Y* (2016) ABI4 represses the expression of type-A ARRs to inhibit seed germination in Arabidopsis.Plant J89: 354-365.
7. Yu F, Lou L, Tian M, Li Q, Ding Y, Wu Y, Belda-Palazon B, Rodrigue P L,Yang S, Xie Q* (2016) ESCRT-I component VPS23A affects ABA signaling by recognizing ABA receptors for endosomal degradation.Mol Plant9: 1570-1582.
8. Ding Y, Li H, Zhang X, Xie Q, Gong Z,Yang S* (2015) OST1 kinase modulates freezing tolerance by enhancing ICE1 stability in Arabidopsis.Dev Cell 32: 278-289. (Previewed in the same issue of Mol Cell)
9. Liu J#, Yang H#, Bao F, Ao K, Zhang X, Zhang Y,Yang S* (2015) IBR5 modulates temperature-dependent, R protein CHS3-mediated defense responses in Arabidopsis.PLoS Genet11, e1005584. (#These authors contributed equally)
10. Kong L, Cheng J, Zhu Y, Ding Y, Meng J, Chen Z, Xie Q, Guo Y, Li J,Yang S, Gong Z* (2015) Degradation of the ABA co-receptor ABI1 by PUB12/13 U-box E3 ligases.Nat Commun6: 8630. Doi: 10.1038/ncomms9630.
11. Ji H, Wang Y, Cloix C, Li K, Jenkin G, Wang S, Shang Z, Shi Y,Yang S, Li X* (2015) The Arabidopsis RCC1 family protein TCF1 regulates freezing tolerance and cold acclimation through modulating lignin biosynthesis.PLoS Genet11, e1005471.
12. Shi Y#, Ding Y#, Yang S* (2015) Cold signal transduction and its interplay with phytohormones during cold acclimation. Plant Cell Physiol56: 7-15. (#These authors contributed equally)
13. Bao F, Huang X, Zhu C, Zhang X, Li X, Yang S*(2014) Arabidopsis HSP90 protein modulates RPP4-mediated temperature-dependent cell death and defense responses. New Phytol202: 1320-1334.
14. Song S, Huang H, Gao H, Wang J, Wu D, Liu X, Yang S, Zhai Q, Li C, Qi T, Xie D* (2014) Interaction between MYC2 and ETHYLENE INSENSITIVE3 modulates antagonism between jasmonate and ethylene signaling in Arabidopsis. Plant Cell26: 263-279.
15. Wang Y, Zhang Y, Wang Z, Zhang X,Yang S*(2013) A missense mutation in CHS1, a TIR-NB protein, induces chilling sensitivity in Arabidopsis. Plant J 75: 553-565.
16. Shi Y, Wang Z, Meng P, Tian S, Zhang X, Yang S* (2013) The glutamate carboxypeptidase AMP1 mediates ABA and abiotic stress responses in Arabidopsis. New Phytol 199: 135-150.
17. Guo L, Yang H, Zhang X, Yang S*(2013) Lipid transfer protein 3 as a target of MYB96 mediates freezing and drought stress in Arabidopsis. J Exp Bot64: 1755-1767.
18. Li J#, Mu J#, Bai J#, Fu F, Zou T, An F, Zhang J, Jing H, Wang Q, Yang S, Zuo J* (2013) PARAQUAT RESISTANT 1, a Golgi-localized putative transporter protein, is involved in intracellular transport of paraquat. Plant Physiol 162: 470-483. (#These authors contributed equally)
19. Shi Y#, Tian S#, Hou L, Huang X, Zhang X, Guo H, Yang S*(2012) Ethylene signaling negatively regulates freezing tolerance by repressing expression of CBF and type-A ARR genes in Arabidopsis. Plant Cell24: 2578-2595. (#These authors contributed equally)
20. Wang Z#, Meng P#, Zhang X, Ren D,Yang S*(2011) BON1 interacts with the protein kinases BIR1 and BAK1 in modulation of temperature-dependent plant growth and cell death in Arabidopsis.Plant J 67: 1081-1093. (#These authors contributed equally)
21. Huang X, Li J, Bao F, Zhang X,Yang S*(2010) A gain-of-function mutation in the Arabidopsis disease resistance gene RPP4 confers sensitivity to low temperature.Plant Physiol154: 796-809.
22. Yang H, Shi Y, Liu J, Guo L, Zhang X,Yang S*(2010) A mutant CHS3 protein with TIR-NB-LRR-LIM domains modulates growth, cell death and freezing tolerance in a temperature-dependent manner in Arabidopsis.Plant J63: 283-296.
23. Huang X, Li Y, Zhang X, Zuo J,Yang S*(2010) The Arabidopsis LSD1 gene plays an important role in the regulation of low temperature-dependent cell death.New Phytol 187: 301-312 (Cover story).
24. Liu D, Gong Q, Ma Y, Li P, Li J,Yang S, Yuan L, Yu Y, Pan D, Xu F, Wang NN* (2010) cpSecA, a thylakoid protein translocase subunit, is essential for photosynthetic development in Arabidopsis.J Exp Bot 61: 1655-1669.
25. Huang X, Zhang X,Yang S*(2009) A novel chloroplast-localized protein EMB1303 is required for chloroplast development in Arabidopsis.Cell Res19: 1205-1216.
26. Gou M, Su N, Zheng J, Huai J, Wu G, Zhao J, He J, Tang D,Yang S, Wang G* (2009) An F-box gene, CPR30, functions as a negative regulator of the defense response in Arabidopsis.Plant J 60: 757-770.
27. Li Y#,Yang S#, Yang H, Hua J* (2007) The TIR-NB-LRR gene SNC1 is regulated at the transcript level by multiple factors.Mol Plant Microbe Interact20: 1449-1456. (#These authors contributed equally)
28. Yang H,Yang S, Li Y, Hua J* (2007) The Arabidopsis BAP1 and BAP2 genes are general inhibitors of programmed cell death.Plant Physiol 145: 135-146.
29. Chai MF, Wei PC, Chen QJ, An R, Chen YM, Chen J,Yang S*, Wang XC* (2006) NADK3, a novel cytoplasmic source of NADPH, is required under conditions of oxidative stress and modulates abscisic acid responses in Arabidopsis.Plant J47: 665-674.
30.Yang S, Yang H, Grisafi P, Sanchatjate S, Fink GR, Sun Q, Hua J* (2006) The BON/CPN gene family represses cell death and promotes cell growth in Arabidopsis.Plant J 45: 166-179.
31.YangS, and Hua J* (2004) A haplotype-specific resistance gene regulated by BONZAI1 mediates temperature-dependent growth control in Arabidopsis.Plant Cell16: 1060-1071.
32.Yang S*, Yu H, Xu Y, Goh CJ* (2003) Investigation of cytokinin-deficient phenotypes in Arabidopsis by ectopic expression of orchid DSCKX1.FEBS Lett 555: 291-296.
33.Yang S, Yu H, Goh CJ* (2003) Functional characterisation of a cytokinin oxidase gene DSCKX1 in Dendrobium orchid. Plant Mol Biol 51: 237-248.
34.Yang S, Yu H, Goh CJ* (2002) Isolation and characterization of the orchid cytokinin oxidase DSCKX1 promoter.J Exp Bot 53: 1899-1907.
35. Yu H,Yang S, Goh CJ* (2002) Spatial and temporal expression of the orchid floral homeotic gene DOMADS1 is mediated by its upstream regulatory elements.Plant Mol Biol49: 225-237.
36. Yu H,Yang S, Goh CJ* (2001) Agrobacterium-mediated transformation of a Dendrobium orchid with the class 1 knox gene DOH1.Plant Cell Rep 20: 301-305.
37. Yu H,Yang S, Goh CJ* (2000) DOH1, a class 1 knox Gene, is required for maintenance of the basic plant architecture and floral transition in orchid.Plant Cell 12: 2143-2160.
38. 丁楊林，施怡婷，楊淑華* (2015) 植物回響低溫脅迫的分子機制研究. 生命科學. 27: 398-405.
39. 鄧 岩，王興春，楊淑華*，左建儒* (2006) 細胞分裂素：代謝、信號轉導、交叉反應與農藝性狀改良. 植物學通報. 23: 478-498.
專著
Shi Y,Yang S*(2014) ABA regulation of the cold stress response in plants. In Zhang D (ed)Abscisic Acid: Metabolism, Transport and Signaling.Springer, pp337-363.
Yu H, Dhavale T,Yang S(2006) Molecular mechanisms of hormone functions in flowering In Teixeira da Silva JA (ed) Floriculture, Ornamental and Plant Biotechnology: Advances and Topical Issues, Ed 1 Vol 1 Global Science Books, London, UK, pp 25-32.
任勃，王興春，馮健，楊淑華，左建儒 (2012) 細胞分裂素. 植物激素作用的分子機理 (許智宏，薛紅衛主編) 上海科學技術出版社.
武維華，張蜀秋，袁明，張軍，韓玉珍，李穎章，葉德，楊淑華，王學臣 (2008) 植物生理學 (第二版) 科學出版社.
