人物經歷
1982年2月於遼寧師範學院獲理學學士學位
1984年10月於東北師範大學遺傳學專業獲理學碩士學位
1989年6月於東北師範大學植物學專業獲理學博士學位。
1984年10月至1998年3月在遼寧師範大學任教,1996年晉升教授。
1998年3月,調入大連理工大學任教至今,曾任生物工程系主任,研究所所長。
1995年獲遼寧省優秀青年科技工作者稱號。
1996年獲遼寧省首屆青年科研人才基金。
1999年獲大連市優秀人才獎。
2001年獲大連市優秀專家稱號。
2001年被評為遼寧省第二批百千萬人才工程百人層次人選。
2004年擔任遼寧省生物技術協會專家委員會專家
研究領域
1. 植物轉基因工程與育種技術平台研究。編號:JY03-B-18,國家科技部植物轉基因重大專項,100萬元,2003-2004
2. 基因工程關鍵技術。項目編號:2001101001,遼寧省科技廳,210萬元,2001-2004
3. 無載體、無標記基因植物轉基因技術。項目編號:2003B4NS015,大連市科技局,100萬元,2003-2004
4. 蛇島蝮蛇類凝血酶基因的分離和表達。遼寧省科技攻關,10萬元,1999.01-2001.12
5. 蛇島蝮蛇類凝血酶基因的分離和表達。大連市科委,15萬元,1998-2001
6. 胸腺素α1基因工程藥物開發。通化金馬製藥股份公司,390萬元,1998.01~2001.12
7. 楊樹基因工程育種。鞍山生生高新植物技術套用股份有限公司,25萬元,2001.9-2003.9
8. 長春花鹼單體的分離研究。大連理工大學領先集團有限公司,20萬元,2002.7-2003.7
9. 真福康保健功效的驗證與鑑定。大連理工大學領先集團有限公司,10萬元,2002.7-2003.7
指導碩、博士生研究方向:植物基因工程、生物製藥
出版著作
五年來共發表文章120餘篇,SCI收錄42篇,主要文章包括:
1. Properties of A. ficuum AS3.324 phytase expressed in tobacco. Process Biochemistry,2005,40:213–216 SCI
2. Purification,characterization and gene cloning of a novel phospholipase A2 from the venom of Agkistrodon blomhoffii ussurensis. The International Journal of Biochemical & Cell Biology. 2005,37:558-565 SCI
3. Neuroprotective properties of catalpol in transient global cerebral ischemia in gerbils: dose-response,therapeutic time-window and long-term efficacy. Brain Research,2004,1029:179-185 SCI
4. Plasmodium falciparum histone acetyltransferase,a yeast GCN5 homologue involved in chromatin remodeling. Eukaryotic Cell,2004,3(2):264-276
5. Catalpol inhibits apoptosis in hydrogen peroxide-induced PC12 cells by preventing cytochrome c release and inactivating of caspase casecade. Toxicon,2004,43:53-59
6. PfADA2,a Plasmodium falciparum homologue of the transcriptional coactivator ADA2 and its in vivo association with the histone acetyltransferase PfGCN5. Gene,2004,336:251-261
7. Construction of Targeting Vector and Expression of Green Fluorescent Protein in the Silkworm,Antheraea pernyi. DNA and Cell Biology. 2003,22:441-446
8. The Expression of GFP Under the Control of Fibroin Promotor in Primary ovarian cells of Antheraea pernyi. Journal of Biosciences,2003,28(6):691-695
9. 菟絲子提取物在PC12細胞株中的神經營養因子樣活性。生物化學與生物物理進展。2003,30(2):226-230
10. Codonopsis pilosula (Franch) Nannf total alkaloids potentiate neurite outgrowth induced by nerve growth factor in PC12 cells. Acta Pharmacologica Sinica,2003,24(9):913-917
11. Effect of Cuscuta chinensis glycoside on the neuronal differentiation of rat pheochromocytoma PC12 cells. International Journal of Developmental Neuroscience,2003,21:277-281
12. Hydrogen peroxide-induced apoptosis in PC12 cells and the protective effect of puerarin. Cell Biology International,2003,27:1025-1031
13. Neuroprotective effect of Alpinia oxyphylla Miq. fruits against glutamate-induced apoptosis in cortical neurons. Toxicology Letters,2003,144(2):205–212
14. Neuroprotective effect of Alpinia oxyphylla extract against glutamate excitotoxicity in cultured mouse cortical neurons. Neurosci Res Commun,2003,33(2):105–113
15. Investigation of the neuronal death mode induced by glutamate treatment in serum-,antioxidant-free primary cultured cortical neurons. Developmental Brain Research,2003,145(2):263-268
16. A new way for chemical degradation of plastic by natural volatile constituents of Ledum palustre. Chinese Science Bulletin,2003,48(16):1718-1721
17. Enhancing ethanol tolerance of a self-flocculating fusant of Schizosaccharomyces pombe and Saccharomyces cerevisiae by Mg2+ via reduction in plasma membrane permeability. Biotechnology Letters,2003,25:1191-1194
18. Dextromethorphan protects dopaminergic neurons against inflammation-mediated degeneration through inhibition of microglial activation. The Journal Of Pharmacology and Experimental Therapeutics,2003,305(1):212-218
19. 酸性水溶液中硫氰酸鉬(V)配合物的研究。光譜學與光譜分析,2003,23(2)337-339
20. Purification and characterization of ulva pertusa kjellm alkaline phosphatase. Preparative Biochemistry & Biotechnology,2003,33(2):113-123
21. Cloning of cDNA Encoding Choline Monooxygenase from Suaeda liaotungensis and Salt Tolerance of Transgenic Tobacco. Acta Botanica Sinica,2003,45(2):242-247
22. Molecular cloning and characterization of betaine aldehyde dehydrogenase gene from Suaeda liaotungensis and its use in improved tolerance to salinity in transgenic tobacco. Biotechnol Lett. 2003,25(17):1431-1436
23. Characterization of Sporothric schenckii by random amplication of polymorphic DNA assay. Chinese Medicine Journal,2003,116(2):239-242
24. Expression of gloshedobin,a thrombin-like enzyme from the venom of Gloydius shedaoensis,in Escherichia coli. Biotechnology Letters,2003,25(2):101–104
25. High-level expression of a soluble snake venom enzyme,gloshedobin,in E. coli in the presence of metal ions. Biotechnology Letters,2003,25(8):607–610
26. Purification and characterization of ginsenoside--arabinofuranase hydrolyzing ginsenoside Rc into Rd from the fresh root of Panax ginseng. Process Biochemistry,2002,37:793-798
27. Cloning and Expression of defibrase cDNA from the venom of Gloydius shedaoensis. Biotechnology Letters,2002,24:135-138
28. Cloning Expression and Purification of Gussrobin A Thrombin-like Enzyme from the Snake Venom of Gloydius ussuriensis. Prog Biochem Biophys. 2002,34(1):6-10
29. Expression,Purification and Partial Characterization of Recombinant Defibrase,a Thrombin-Like Enzyme from the Venom of Glyodius shedaoensis. Prog Biochem Biophys. 2002,29(3)390-393
30. Production of a Functional Single-chain Fv Protein in Transgenic Tobacco Root Exudates. Biotechnology letters. 2002,24(18):1531-1534
31. An expeditious method for constructing T-vectors using Eam 1105 Ⅰ cassettes. Plant Molecular Biology Reporter,2002,20:189a-189e
32. Application of DNA sequencing in detection of telomerase activity. Biochemical Engineering Journal. 2002,11(2-3):79-85
33. Production of high affinity human single-chain antibody against preS1 of Hepatitis B Virus: comparison of large naïve and in vitro immune phage displayed antibody library. Progressing in Biochemistry and Biophysics. 2002,29(4):572-575
34. Inhibition by naloxone stereoisomers of beta-amyloid peptide (1-42)-induced superoxide production in microglia and degeneration of cortical and mesencephalic neurons. Journal of Pharmacology and Experimental Therapeutics,2002,302(3):1212-1219
35. A Serum- and Antioxidant-free Primary Culture Model of Mouse Cortical Neurons for Pharmacological Screen and Studies of Neurotrophic and Neuroprotective Agents. Cellular and Molecular Neurobiology. 2002,22(2):197-206
36. Expression of endoplasmic reticulum molecular chaperon GRP94 in human lung cancer tissues and its clinical significance. Chinese Medical Journal,2002,115(11):1615-1619
37. Embryonic stem cell as nuclear donor could promote in vitro development of the heterogeneous reconstructed embryo. Chinese Science Bulletin,2002,47(21):1811-1815
38. 銅鋅超氧化物歧化酶與色氨酸鈷(Ⅱ)的相互作用。光譜學與光譜分析,2000,3:305-307
39. 紫外-可見吸收光譜法測定配合顯色反應的焓變。光譜學與光譜分析,2001,21(4):527-528
40. Kinetics of Inactivation of Ulva pertusa Kjellm Alkaline Phosphatase by Ethylenediaminetetraacetic Acid Disodium. Journal of Enzyme Inhibition,2001,16:313-319
41. Purification and Characterization of ginsenoside-?-Glucosidase from Ginseng. Chem. Pharm. Bull,2001,49(7):795-798
42. 分光光度法研究EDTA對小牛腸鹼性磷酸酶的不可逆抑制動力。光譜學與光譜分析,2001,21(5):701-703
43. 鹼性磷酸梅與Cu (Ⅱ) 相互作用的譜學研究。光譜學與光譜分析,2001,21(4):432-434
44. 氨基醯化酶與Cu(Ⅱ)相互作用的譜學研究。光譜學與光譜分析,2001,21(6):804-806
45. 可見光譜學研究核酸酶P1與氯化銅(Ⅱ)的相互作用。光譜學與光譜分析,2000,20(3):308-310
46. 大連蛇島蝮蛇類凝血酶基因克隆與表達研究。大連理工大學學報,2003,43(2):156-159
47. 新的重組蛋白表達系統——植物根分泌和葉分泌。生物化學與生物物理進展,2003,30(1):128
48. 抗B肝病毒表面抗原PreS1(20-47)的單鏈抗體在轉基因菸草根分泌物中的初步表達。高技術通訊,2003,13(9):35-38
49. Variation and characterization analysis of partial fragment of fibroin gene from silkworm,Antheraea pernyi. High Technology Letters,2003,9(3):29-32
50. 喜樹鹼生物合成途徑及其代謝調控的研究進展。高技術通訊,2004,14(5):106-110
51. 菟絲子提取物對PC12細胞中GAP-43表達影響。大連理工大學學報,2004,44(3):378-382
52. Rapid Amplification of 5′cDNA End of Suaeda liaotungensis Choline Monooxygenase Using Inverse PCR RACE. High Technol Lett 2002,8(1):5-7
53. Separation and purification of betaine aldehyde dehydrogenase from wild Suaeda liaotungensis. High Technology Letters,2002,8(2):5-17
54. Cloning a Full-length cDNA Encoding UDP-glucose Pyrophosphorylase from Amorpha Fruticosa by PCR-Based Methods. High Technology Letters,2002,8(3):5-10
55. An Lijia. A co-expression system based on phage and phagemid to select cognate antibody-antigen pairs in vivo. High Technology Letters,2002,8(2):5-10
56. Construction of Large Human Single-chain Antibody Phage Display Library. High Technology Letters,2002,8(3):1-4
57. 精子幹細胞轉染法製備轉基因兔的研究。高技術通訊,2001,11(130):17-21
58. 反向嵌套PCR高效擴增遼寧鹼蓬甜菜鹼醛脫氫酶cDNA 5’末端序列。高技術通訊,2001,1(11):17-19
59. Research on telomerase detection of activity by combining DNA sequence analysis with TRAP. High Technology Letters,2001,7(3):8-10
60. 新型染料配基對鹼性磷酸酶的親合純化。高校化學工程學報,2001,5(6):563-567
61. Separation and Purification of Thrombin-like Enzyme by Affinity Adsorbents. High Technology Letters,2001,7(4):13-15
62. 卵黃免疫球蛋白配基親和純化鹼性磷酸酶。中國農業科學,2004,37(6):908-911
相關圖書
英漢生物技術辭彙
作者:安利佳等 ISBN:750253978
出版社:化學工業出版社 出版日期:2003-01-01
科研成果
共承擔國家、省、市課題10項,項目經費總計900萬元;
申報國內外專利13項,其中國際專利1項;
由安利佳教授為負責人完成的“一種植物分子育種新技術的建立與套用”項目鑑定會,於2004年12月19日通過了遼寧省科技廳科技成果鑑定,達到了國際領先水平。
所主持的遼寧省科技廳項目“基因工程關鍵技術”和大連市科技局項目“無載體、無標記基因植物轉基因技術”於2004年4月6日通過了遼寧省科技廳科技成果驗收和大連市科技局科技成果鑑定,達到了國際先進水平。
獲得榮譽
2004年安利佳教授率領的課題組被學校評為大連理工大學科研創新團隊。